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Induction of Osteogenic MC3T3-E1 Cell Differentiation by Nacre and Flesh Lipids of Tunisian Pinctada radiata.

Abstract

The flesh of the Pinctada radiata pearl oyster from coastal Tunisia is considered as a high source of n-3 and n-6 and its shell nacre layer is a promising osteogenic biomaterial. Fatty acid (FA) analysis showed that the major components found in total FA (TFA) were 14:0, 16:0, and 18:0 saturated FA (SFA); 16:1, 18:1, and 20:1 monoenoic FA; 20:4n-6 (ARA), 22:5n-3 (DPA). Characteristically high levels of 20:5n-3 (EPA) and 22:6n-3 (DHA) (6.53-89.75 mg/100 g TFA) polyunsaturated FA (PUFA) were found, respectively, in the TFA of nacre and flesh. Evaluated the effects in vitro of lipids extracted from nacre (Ln) and from flesh (Lc) of P. radiata on growth and the differentiation of osteoblasts. Cytotoxicity tests (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide [MTT] and lactic acid dehydrogenase c [LDH]) demonstrated that both extracts are nontoxic. Alizarin Red staining was used in an osteoblast differentiation model using the osteoblast MC3T3-E1 cell line. It showed that the FA of both extracts induced osteoblast differentiation leading to mineralization. Reverse transcription-polymerase chain reaction (RT-PCR) showed a significantly higher expression of osteocalcin (Bglap) and runt-related transcription (Runx2) in MC3T3-E1 cells in the presence of Ln. No difference of osteopontin (Spp1) and Collagen type I (Col1a1) genes compared to the control was observed. In conclusion, these results supported, obtained from our in vitro experimental model used, the interest/potential of lipids extracted from nacre and P. radiata flesh to stimulate bone formation.

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  • Authors+Show Affiliations

    ,

    IMoPA, UMR 7365, FMN, CNRS Université de Lorraine, 9 av. de la forêt de Haye, 54505 Vandoeuvre-lès-, Nancy, France. UR 13 ES 35, FST. Université de Tunis El Manar, Campus Universitaire EL Manar I, 1060, Tunis, Tunisie.

    ,

    IMoPA, UMR 7365, FMN, CNRS Université de Lorraine, 9 av. de la forêt de Haye, 54505 Vandoeuvre-lès-, Nancy, France.

    ,

    IMoPA, UMR 7365, FMN, CNRS Université de Lorraine, 9 av. de la forêt de Haye, 54505 Vandoeuvre-lès-, Nancy, France.

    ,

    UR 13 ES 35, FST. Université de Tunis El Manar, Campus Universitaire EL Manar I, 1060, Tunis, Tunisie.

    ,

    UR 13 ES 35, FST. Université de Tunis El Manar, Campus Universitaire EL Manar I, 1060, Tunis, Tunisie.

    ,

    IMoPA, UMR 7365, FMN, CNRS Université de Lorraine, 9 av. de la forêt de Haye, 54505 Vandoeuvre-lès-, Nancy, France.

    ,

    UR 13 ES 35, FST. Université de Tunis El Manar, Campus Universitaire EL Manar I, 1060, Tunis, Tunisie.

    IMoPA, UMR 7365, FMN, CNRS Université de Lorraine, 9 av. de la forêt de Haye, 54505 Vandoeuvre-lès-, Nancy, France.

    Source

    Lipids : 2019 Jun 17 pg

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31206721

    Citation

    Rym, Ben Ammar, et al. "Induction of Osteogenic MC3T3-E1 Cell Differentiation By Nacre and Flesh Lipids of Tunisian Pinctada Radiata." Lipids, 2019.
    Rym BA, Marie-Hélène P, Alice B, et al. Induction of Osteogenic MC3T3-E1 Cell Differentiation by Nacre and Flesh Lipids of Tunisian Pinctada radiata. Lipids. 2019.
    Rym, B. A., Marie-Hélène, P., Alice, B., Khaoula, T., Rim, W., Marthe, R., ... Pierre, G. (2019). Induction of Osteogenic MC3T3-E1 Cell Differentiation by Nacre and Flesh Lipids of Tunisian Pinctada radiata. Lipids, doi:10.1002/lipd.12141.
    Rym BA, et al. Induction of Osteogenic MC3T3-E1 Cell Differentiation By Nacre and Flesh Lipids of Tunisian Pinctada Radiata. Lipids. 2019 Jun 17; PubMed PMID: 31206721.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Induction of Osteogenic MC3T3-E1 Cell Differentiation by Nacre and Flesh Lipids of Tunisian Pinctada radiata. AU - Rym,Ben Ammar, AU - Marie-Hélène,Piet, AU - Alice,Brion, AU - Khaoula,Telahigue, AU - Rim,Werheni, AU - Marthe,Rousseau, AU - Mhamed,El Cafsi, AU - Pierre,Gillet, Y1 - 2019/06/17/ PY - 2018/05/06/received PY - 2019/02/09/revised PY - 2019/02/11/accepted PY - 2019/6/18/entrez PY - 2019/6/18/pubmed PY - 2019/6/18/medline KW - Flesh KW - Lipids KW - MC3T3-E1 KW - Nacre (mother of pearl) KW - Pinctada radiata JF - Lipids JO - Lipids N2 - The flesh of the Pinctada radiata pearl oyster from coastal Tunisia is considered as a high source of n-3 and n-6 and its shell nacre layer is a promising osteogenic biomaterial. Fatty acid (FA) analysis showed that the major components found in total FA (TFA) were 14:0, 16:0, and 18:0 saturated FA (SFA); 16:1, 18:1, and 20:1 monoenoic FA; 20:4n-6 (ARA), 22:5n-3 (DPA). Characteristically high levels of 20:5n-3 (EPA) and 22:6n-3 (DHA) (6.53-89.75 mg/100 g TFA) polyunsaturated FA (PUFA) were found, respectively, in the TFA of nacre and flesh. Evaluated the effects in vitro of lipids extracted from nacre (Ln) and from flesh (Lc) of P. radiata on growth and the differentiation of osteoblasts. Cytotoxicity tests (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide [MTT] and lactic acid dehydrogenase c [LDH]) demonstrated that both extracts are nontoxic. Alizarin Red staining was used in an osteoblast differentiation model using the osteoblast MC3T3-E1 cell line. It showed that the FA of both extracts induced osteoblast differentiation leading to mineralization. Reverse transcription-polymerase chain reaction (RT-PCR) showed a significantly higher expression of osteocalcin (Bglap) and runt-related transcription (Runx2) in MC3T3-E1 cells in the presence of Ln. No difference of osteopontin (Spp1) and Collagen type I (Col1a1) genes compared to the control was observed. In conclusion, these results supported, obtained from our in vitro experimental model used, the interest/potential of lipids extracted from nacre and P. radiata flesh to stimulate bone formation. SN - 1558-9307 UR - https://www.unboundmedicine.com/medline/citation/31206721/Induction_of_Osteogenic_MC3T3-E1_Cell_Differentiation_by_Nacre_and_Flesh_Lipids_of_Tunisian_Pinctada_radiata L2 - https://doi.org/10.1002/lipd.12141 DB - PRIME DP - Unbound Medicine ER -