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Transcriptome analysis reveals differentially expressed genes and pathways for oviduct development and defense in prelaying and laying hens.
Am J Reprod Immunol 2019; 82(3):e13159AJ

Abstract

PROBLEM

The oviduct plays an indispensable role in the formation of eggs, especially the magnum and uterus. The identification of oviduct development in different stages will help to target candidate genes and pathways in regulation of albumen and eggshell formation, as well as defense mechanism in oviduct and egg.

METHODS

To identify the function differences and the molecular defense mechanism of the oviduct and egg, we performed transcriptome sequencing analysis of the magnum and uterus in 120-d-old and 300-d-old Lohmann layers, three birds in each group.

RESULTS

With fold changes (log2 ratio) ≥ 2 and false discovery rate (FDR) < 0.01, RNA-Seq revealed 1,040 genes expressed differentially in the magnum and 595 genes in the uterus. By combining GO enrichment and KEGG pathway analysis, it served to show that gene activities of the magnum and uterus in prelaying chickens were more likely to concentrate on growth and development, and after egg-laying, they were mainly inclined to enhance the substances transmembrane transport and secretion activities. We further characterized 1579 new genes, while only 803 of them were functionally annotated. A complex mixture of proteins related to defense was measured in this study. A subset of avian β-defensins (AvBDs) and ovodefensins (OvoDs), that is, AvBD12, AvBD11, AvBD10, OvoDA1, OvoDB1, OvoDA2, OvoDA3, and OvoDBβ, was detected to express in the magnum of laying hens at high levels.

CONCLUSION

Collectively, the identification and functional analysis of these differentially expressed genes (DEGs), as well as specific expression of avian defensins, may contribute to understand the development and defense mechanisms of oviduct and eggs.

Authors+Show Affiliations

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China. Guangxi Agricultural Vocational College, Nanning, China.Institute of Ecology, Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, China.Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.Sichuan Animal Science Academy, Chengdu, China. Animal Breeding and Genetics Key Laboratory of Sichuan Province, Chengdu, China.Sichuan Animal Science Academy, Chengdu, China. Animal Breeding and Genetics Key Laboratory of Sichuan Province, Chengdu, China.Sichuan Animal Science Academy, Chengdu, China. Animal Breeding and Genetics Key Laboratory of Sichuan Province, Chengdu, China.Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31206849

Citation

Yin, Lingqian, et al. "Transcriptome Analysis Reveals Differentially Expressed Genes and Pathways for Oviduct Development and Defense in Prelaying and Laying Hens." American Journal of Reproductive Immunology (New York, N.Y. : 1989), vol. 82, no. 3, 2019, pp. e13159.
Yin L, Yu L, Zhang L, et al. Transcriptome analysis reveals differentially expressed genes and pathways for oviduct development and defense in prelaying and laying hens. Am J Reprod Immunol. 2019;82(3):e13159.
Yin, L., Yu, L., Zhang, L., Ran, J., Li, J., Yang, C., ... Liu, Y. (2019). Transcriptome analysis reveals differentially expressed genes and pathways for oviduct development and defense in prelaying and laying hens. American Journal of Reproductive Immunology (New York, N.Y. : 1989), 82(3), pp. e13159. doi:10.1111/aji.13159.
Yin L, et al. Transcriptome Analysis Reveals Differentially Expressed Genes and Pathways for Oviduct Development and Defense in Prelaying and Laying Hens. Am J Reprod Immunol. 2019;82(3):e13159. PubMed PMID: 31206849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome analysis reveals differentially expressed genes and pathways for oviduct development and defense in prelaying and laying hens. AU - Yin,Lingqian, AU - Yu,Lintian, AU - Zhang,Long, AU - Ran,Jinshan, AU - Li,Jingjing, AU - Yang,Chaowu, AU - Jiang,Xiaosong, AU - Du,Huarui, AU - Hu,Xiaofang, AU - Liu,Yiping, Y1 - 2019/07/03/ PY - 2019/03/15/received PY - 2019/06/01/revised PY - 2019/06/06/accepted PY - 2019/6/18/pubmed PY - 2019/6/18/medline PY - 2019/6/18/entrez KW - defense mechanism KW - egg white KW - eggshell KW - magnum KW - transcriptome KW - uterus SP - e13159 EP - e13159 JF - American journal of reproductive immunology (New York, N.Y. : 1989) JO - Am. J. Reprod. Immunol. VL - 82 IS - 3 N2 - PROBLEM: The oviduct plays an indispensable role in the formation of eggs, especially the magnum and uterus. The identification of oviduct development in different stages will help to target candidate genes and pathways in regulation of albumen and eggshell formation, as well as defense mechanism in oviduct and egg. METHODS: To identify the function differences and the molecular defense mechanism of the oviduct and egg, we performed transcriptome sequencing analysis of the magnum and uterus in 120-d-old and 300-d-old Lohmann layers, three birds in each group. RESULTS: With fold changes (log2 ratio) ≥ 2 and false discovery rate (FDR) < 0.01, RNA-Seq revealed 1,040 genes expressed differentially in the magnum and 595 genes in the uterus. By combining GO enrichment and KEGG pathway analysis, it served to show that gene activities of the magnum and uterus in prelaying chickens were more likely to concentrate on growth and development, and after egg-laying, they were mainly inclined to enhance the substances transmembrane transport and secretion activities. We further characterized 1579 new genes, while only 803 of them were functionally annotated. A complex mixture of proteins related to defense was measured in this study. A subset of avian β-defensins (AvBDs) and ovodefensins (OvoDs), that is, AvBD12, AvBD11, AvBD10, OvoDA1, OvoDB1, OvoDA2, OvoDA3, and OvoDBβ, was detected to express in the magnum of laying hens at high levels. CONCLUSION: Collectively, the identification and functional analysis of these differentially expressed genes (DEGs), as well as specific expression of avian defensins, may contribute to understand the development and defense mechanisms of oviduct and eggs. SN - 1600-0897 UR - https://www.unboundmedicine.com/medline/citation/31206849/Transcriptome_Analysis_Reveals_Differentially_Expressed_Genes_and_Pathways_for_Oviduct_Development_and_Defense_in_Prelaying_and_Laying_Hens L2 - https://doi.org/10.1111/aji.13159 DB - PRIME DP - Unbound Medicine ER -