Tags

Type your tag names separated by a space and hit enter

Paeoniflorin protects spiral ganglion neurons from cisplatin-induced ototoxicity: Possible relation to PINK1/BAD pathway.
J Cell Mol Med 2019; 23(8):5098-5107JC

Abstract

The objective of this study was to elucidate whether paeoniflorin (PF) exerted an effect on cisplatin-induced spiral ganglion neuron (SGN) damage, with special attention given to the role of PINK1/BAD pathway in this process. Middle cochlear turn culture and C57BL/6 mice were utilized to identify the character of PF in vitro and in vivo. We found that cisplatin treatment led to SGN damage, in which reactive oxygen species (ROS) generation increased, PINK1 expression decreased, BAD accumulation on mitochondria raised and mitochondrial apoptotic pathway activated. Conversely, we demonstrated that PF pre-treatment obviously mitigated cisplatin-induced SGN damage. Mechanistic studies showed that PF could reduce ROS levels, increase PINK1 expression, decrease the BAD accumulation on mitochondria and, thus, alleviate the activated mitochondrial apoptosis in SGNs caused by cisplatin. Overall, the findings from this work reveal the important role of PF and provide another strategy against cisplatin-induced ototoxicity.

Authors+Show Affiliations

Shandong Provincial ENT Hospital affiliated to Shandong University, Jinan, China.Department of Otolaryngology Head and Neck Surgery, Zibo Central Hospital, Zibo, China.Shandong Provincial ENT Hospital affiliated to Shandong University, Jinan, China.Shandong Provincial ENT Hospital affiliated to Shandong University, Jinan, China.Shandong Provincial ENT Hospital affiliated to Shandong University, Jinan, China.Shandong Provincial ENT Hospital affiliated to Shandong University, Jinan, China.Shandong Provincial ENT Hospital affiliated to Shandong University, Jinan, China.Department of Histology and Embryology, College of basic Medicine, Jining Medical University, Jinan, China.Shandong Provincial ENT Hospital affiliated to Shandong University, Jinan, China.Shandong Provincial ENT Hospital affiliated to Shandong University, Jinan, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31207045

Citation

Yu, Xiaoyu, et al. "Paeoniflorin Protects Spiral Ganglion Neurons From Cisplatin-induced Ototoxicity: Possible Relation to PINK1/BAD Pathway." Journal of Cellular and Molecular Medicine, vol. 23, no. 8, 2019, pp. 5098-5107.
Yu X, Man R, Li Y, et al. Paeoniflorin protects spiral ganglion neurons from cisplatin-induced ototoxicity: Possible relation to PINK1/BAD pathway. J Cell Mol Med. 2019;23(8):5098-5107.
Yu, X., Man, R., Li, Y., Yang, Q., Li, H., Yang, H., ... Wang, H. (2019). Paeoniflorin protects spiral ganglion neurons from cisplatin-induced ototoxicity: Possible relation to PINK1/BAD pathway. Journal of Cellular and Molecular Medicine, 23(8), pp. 5098-5107. doi:10.1111/jcmm.14379.
Yu X, et al. Paeoniflorin Protects Spiral Ganglion Neurons From Cisplatin-induced Ototoxicity: Possible Relation to PINK1/BAD Pathway. J Cell Mol Med. 2019;23(8):5098-5107. PubMed PMID: 31207045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Paeoniflorin protects spiral ganglion neurons from cisplatin-induced ototoxicity: Possible relation to PINK1/BAD pathway. AU - Yu,Xiaoyu, AU - Man,Rongjun, AU - Li,Yanan, AU - Yang,Qianqian, AU - Li,Hongrui, AU - Yang,Huiming, AU - Bai,Xiaohui, AU - Yin,Haiyan, AU - Li,Jianfeng, AU - Wang,Haibo, Y1 - 2019/06/17/ PY - 2018/09/26/received PY - 2019/03/24/revised PY - 2019/04/28/accepted PY - 2019/6/18/pubmed PY - 2019/6/18/medline PY - 2019/6/18/entrez KW - BAD KW - PINK1 KW - ROS KW - paeoniflorin KW - spiral ganglion neuron SP - 5098 EP - 5107 JF - Journal of cellular and molecular medicine JO - J. Cell. Mol. Med. VL - 23 IS - 8 N2 - The objective of this study was to elucidate whether paeoniflorin (PF) exerted an effect on cisplatin-induced spiral ganglion neuron (SGN) damage, with special attention given to the role of PINK1/BAD pathway in this process. Middle cochlear turn culture and C57BL/6 mice were utilized to identify the character of PF in vitro and in vivo. We found that cisplatin treatment led to SGN damage, in which reactive oxygen species (ROS) generation increased, PINK1 expression decreased, BAD accumulation on mitochondria raised and mitochondrial apoptotic pathway activated. Conversely, we demonstrated that PF pre-treatment obviously mitigated cisplatin-induced SGN damage. Mechanistic studies showed that PF could reduce ROS levels, increase PINK1 expression, decrease the BAD accumulation on mitochondria and, thus, alleviate the activated mitochondrial apoptosis in SGNs caused by cisplatin. Overall, the findings from this work reveal the important role of PF and provide another strategy against cisplatin-induced ototoxicity. SN - 1582-4934 UR - https://www.unboundmedicine.com/medline/citation/31207045/Paeoniflorin_protects_spiral_ganglion_neurons_from_cisplatin-induced_ototoxicity:_Possible_relation_to_PINK1/BAD_pathway L2 - https://doi.org/10.1111/jcmm.14379 DB - PRIME DP - Unbound Medicine ER -