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Signal enhancement on gold nanoparticle-based lateral flow tests using cellulose nanofibers.

Abstract

Lateral flow paper-based biosensors merge as powerful tools in point-of-care diagnostics since they are cheap, portable, robust, selective, fast and easy to use. However, the sensitivity of this type of biosensors is not always as high as required, often not permitting a clear quantification. To improve the colorimetric response of standard lateral flow strips (LFs), we have applied a new enhancement strategy that increases the sensitivity of LFs based on the use of cellulose nanofibers (CNF). CNF penetrate inside the pores of LFs nitrocellulose paper, compacting the pore size only in the test line, particularly near the surface of the strip. This modification retains the bioreceptors (antibodies) close to the surface of the strips, and thus further increasing the density of selectively attached gold nanoparticles (AuNPs) in the top part of the membrane, in the test line area, only when the sample is positive. This effect boosts in average a 36.6% the sensitivity of the LFs. The optical measurements of the LFs were carried out with a mobile phone camera whose imaging resolution was improved by attaching microscopic lens on the camera objective. The characterization of CNF into paper and their effect was analyzed using atomic force microscope (AFM) and scanning electron microscope (SEM) imaging techniques.

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  • Authors+Show Affiliations

    ,

    Nanobioelectronics & Biosensors Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193, Barcelona, Spain.

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    Advanced Atomic Force Microscopy Laboratory, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193, Barcelona, Spain.

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    LEPAMAP Research Group, University of Girona, C/Maria Aurèlia Capmany, 61, 17071, Girona, Spain.

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    Nanobioelectronics & Biosensors Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193, Barcelona, Spain.

    ,

    Advanced Atomic Force Microscopy Laboratory, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193, Barcelona, Spain.

    ,

    LEPAMAP Research Group, University of Girona, C/Maria Aurèlia Capmany, 61, 17071, Girona, Spain.

    Nanobioelectronics & Biosensors Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193, Barcelona, Spain; ICREA, Institució Catalana de Recerca I Estudis Avançats, Pg. Lluís Companys 23, 08010, Barcelona, Spain. Electronic address: arben.merkoci@icn2.cat.

    Source

    Biosensors & bioelectronics 141: 2019 Jun 07 pg 111407

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31207571

    Citation

    Quesada-González, Daniel, et al. "Signal Enhancement On Gold Nanoparticle-based Lateral Flow Tests Using Cellulose Nanofibers." Biosensors & Bioelectronics, vol. 141, 2019, p. 111407.
    Quesada-González D, Stefani C, González I, et al. Signal enhancement on gold nanoparticle-based lateral flow tests using cellulose nanofibers. Biosens Bioelectron. 2019;141:111407.
    Quesada-González, D., Stefani, C., González, I., de la Escosura-Muñiz, A., Domingo, N., Mutjé, P., & Merkoçi, A. (2019). Signal enhancement on gold nanoparticle-based lateral flow tests using cellulose nanofibers. Biosensors & Bioelectronics, 141, p. 111407. doi:10.1016/j.bios.2019.111407.
    Quesada-González D, et al. Signal Enhancement On Gold Nanoparticle-based Lateral Flow Tests Using Cellulose Nanofibers. Biosens Bioelectron. 2019 Jun 7;141:111407. PubMed PMID: 31207571.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Signal enhancement on gold nanoparticle-based lateral flow tests using cellulose nanofibers. AU - Quesada-González,Daniel, AU - Stefani,Christina, AU - González,Israel, AU - de la Escosura-Muñiz,Alfredo, AU - Domingo,Neus, AU - Mutjé,Pere, AU - Merkoçi,Arben, Y1 - 2019/06/07/ PY - 2019/03/25/received PY - 2019/05/30/revised PY - 2019/06/02/accepted PY - 2019/6/18/pubmed PY - 2019/6/18/medline PY - 2019/6/18/entrez KW - Biosensor KW - Cellulose KW - Gold nanoparticles KW - Lateral flow KW - Nanofibers SP - 111407 EP - 111407 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 141 N2 - Lateral flow paper-based biosensors merge as powerful tools in point-of-care diagnostics since they are cheap, portable, robust, selective, fast and easy to use. However, the sensitivity of this type of biosensors is not always as high as required, often not permitting a clear quantification. To improve the colorimetric response of standard lateral flow strips (LFs), we have applied a new enhancement strategy that increases the sensitivity of LFs based on the use of cellulose nanofibers (CNF). CNF penetrate inside the pores of LFs nitrocellulose paper, compacting the pore size only in the test line, particularly near the surface of the strip. This modification retains the bioreceptors (antibodies) close to the surface of the strips, and thus further increasing the density of selectively attached gold nanoparticles (AuNPs) in the top part of the membrane, in the test line area, only when the sample is positive. This effect boosts in average a 36.6% the sensitivity of the LFs. The optical measurements of the LFs were carried out with a mobile phone camera whose imaging resolution was improved by attaching microscopic lens on the camera objective. The characterization of CNF into paper and their effect was analyzed using atomic force microscope (AFM) and scanning electron microscope (SEM) imaging techniques. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/31207571/Signal_enhancement_on_gold_nanoparticle-based_lateral_flow_tests_using_cellulose_nanofibers L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(19)30486-5 DB - PRIME DP - Unbound Medicine ER -