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Evaluation of urinary 8-hydroxy-2-deoxyguanosine level in experimental Alzheimer's disease: Impact of carvacrol nanoparticles.
Mol Biol Rep 2019; 46(4):4517-4527MB

Abstract

The present study aimed to compare the effect of carvacrol essential oil and carvacrol nanoemulsion against experimental Alzheimer's (AD). Forty male albino rats were used and divided into four groups as follow: control, AlCl3 induced AD, carvacrol oil treated and carvacrol nanoemulsion treated groups. Brain nor-epinephrine, serotonin and dopamine were analyzed by high performance liquid chromatography (HPLC). Levels of brain Thiobarbituric acid-reactive substances (TBARS), Superoxide dismutase (SOD), reduced glutathione (GSH), cholinesterase, and advanced oxidation protein product (AOPP) were evaluated. Urinary 8-hydroxyguanosine (8-OHdG) level was evaluated by HPLC. Brain Cyclooxygenase 1 and 2 (COX 1and 2) were analyzed by immunohistochemistry. AD induced by AlCl3 in rats was depicted by the significant increase in the neurotransmitters levels which is accompanied with high degree of oxidative stress that was revealed in the elevated level of urinary 8-OHdG along with significant elevation in AOPP, TBARS, and cholinesterase levels and a significant decrease in SOD and GSH; these results are confirmed by immunohistochemistry analysis of COX 1 and 2. On the other hand, the treatment with carvacrol oil and carvacrol nanoemulsion were capable of mitigate effects mediated by AlCl3 administration in treated rats. While the treatment with both approached succeeded to retract the negative impact of AlCl3; but the effect of carvacrol nanoemulsion was more notable than the essential oil. Carvacrol oil and carvacrol nanoemulsion were eminent to overturn AlCl3 induced brain AD which could be imputed to antioxidant and anti-inflammatory capabilities of carvacrol to alter oxidative stress effect. In extension; carvacrol nanoemulsion were evident to give more effective and efficient way in carvacrol delivery to pass through blood brain barriers and ameliorate brain changes.

Authors+Show Affiliations

Medical Biochemistry Department, National Research Centre, 33 El Behouth St, Dokki, Giza, Egypt. dalia_8383@hotmail.com.Biochemistry Division, Chemistry Department, Faculty of Science, Helwan University, Helwan, Egypt.Pre-Treatment and Finishing of Cellulosic Fabric Department, Textile Research Division, National Research Centre, Dokki, Giza, Egypt.Pathology Department, National Research Centre, Dokki, Giza, Egypt.Biochemistry Division, Chemistry Department, Faculty of Science, Helwan University, Helwan, Egypt. Faculty of Medicine, Inserm U1197, Paris Sud-11 University, Paris, France.Medical Biochemistry Department, National Research Centre, 33 El Behouth St, Dokki, Giza, Egypt.Biochemistry Division, Chemistry Department, Faculty of Science, Helwan University, Helwan, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31209743

Citation

Medhat, Dalia, et al. "Evaluation of Urinary 8-hydroxy-2-deoxyguanosine Level in Experimental Alzheimer's Disease: Impact of Carvacrol Nanoparticles." Molecular Biology Reports, vol. 46, no. 4, 2019, pp. 4517-4527.
Medhat D, El-Mezayen HA, El-Naggar ME, et al. Evaluation of urinary 8-hydroxy-2-deoxyguanosine level in experimental Alzheimer's disease: Impact of carvacrol nanoparticles. Mol Biol Rep. 2019;46(4):4517-4527.
Medhat, D., El-Mezayen, H. A., El-Naggar, M. E., Farrag, A. R., Abdelgawad, M. E., Hussein, J., & Kamal, M. H. (2019). Evaluation of urinary 8-hydroxy-2-deoxyguanosine level in experimental Alzheimer's disease: Impact of carvacrol nanoparticles. Molecular Biology Reports, 46(4), pp. 4517-4527. doi:10.1007/s11033-019-04907-3.
Medhat D, et al. Evaluation of Urinary 8-hydroxy-2-deoxyguanosine Level in Experimental Alzheimer's Disease: Impact of Carvacrol Nanoparticles. Mol Biol Rep. 2019;46(4):4517-4527. PubMed PMID: 31209743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of urinary 8-hydroxy-2-deoxyguanosine level in experimental Alzheimer's disease: Impact of carvacrol nanoparticles. AU - Medhat,Dalia, AU - El-Mezayen,Hatem A, AU - El-Naggar,Mehrez E, AU - Farrag,Abdel Razik, AU - Abdelgawad,Mohamed Essameldin, AU - Hussein,Jihan, AU - Kamal,Marina Hanna, Y1 - 2019/06/17/ PY - 2019/03/17/received PY - 2019/06/03/accepted PY - 2019/6/19/pubmed PY - 2019/6/19/medline PY - 2019/6/19/entrez KW - 8-hydroxy-2-deoxyguanosine KW - Advanced oxidation protein product KW - Alzheimer’s disease KW - Carvacrol nanoemulsion KW - Cyclooxygenases KW - Neurotransmitters SP - 4517 EP - 4527 JF - Molecular biology reports JO - Mol. Biol. Rep. VL - 46 IS - 4 N2 - The present study aimed to compare the effect of carvacrol essential oil and carvacrol nanoemulsion against experimental Alzheimer's (AD). Forty male albino rats were used and divided into four groups as follow: control, AlCl3 induced AD, carvacrol oil treated and carvacrol nanoemulsion treated groups. Brain nor-epinephrine, serotonin and dopamine were analyzed by high performance liquid chromatography (HPLC). Levels of brain Thiobarbituric acid-reactive substances (TBARS), Superoxide dismutase (SOD), reduced glutathione (GSH), cholinesterase, and advanced oxidation protein product (AOPP) were evaluated. Urinary 8-hydroxyguanosine (8-OHdG) level was evaluated by HPLC. Brain Cyclooxygenase 1 and 2 (COX 1and 2) were analyzed by immunohistochemistry. AD induced by AlCl3 in rats was depicted by the significant increase in the neurotransmitters levels which is accompanied with high degree of oxidative stress that was revealed in the elevated level of urinary 8-OHdG along with significant elevation in AOPP, TBARS, and cholinesterase levels and a significant decrease in SOD and GSH; these results are confirmed by immunohistochemistry analysis of COX 1 and 2. On the other hand, the treatment with carvacrol oil and carvacrol nanoemulsion were capable of mitigate effects mediated by AlCl3 administration in treated rats. While the treatment with both approached succeeded to retract the negative impact of AlCl3; but the effect of carvacrol nanoemulsion was more notable than the essential oil. Carvacrol oil and carvacrol nanoemulsion were eminent to overturn AlCl3 induced brain AD which could be imputed to antioxidant and anti-inflammatory capabilities of carvacrol to alter oxidative stress effect. In extension; carvacrol nanoemulsion were evident to give more effective and efficient way in carvacrol delivery to pass through blood brain barriers and ameliorate brain changes. SN - 1573-4978 UR - https://www.unboundmedicine.com/medline/citation/31209743/Evaluation_of_urinary_8-hydroxy-2-deoxyguanosine_level_in_experimental_Alzheimer's_disease:_Impact_of_carvacrol_nanoparticles L2 - https://doi.org/10.1007/s11033-019-04907-3 DB - PRIME DP - Unbound Medicine ER -