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Development and validation of a novel SiFaSTRTM 23-plex system.
Electrophoresis. 2019 10; 40(20):2644-2654.E

Abstract

Human identification and paternity testing are usually based on the study of STRs depending on their particular characteristics in the forensic investigation. In this paper, we developed a sensitive genotyping system, SiFaSTR™ 23-plex, which is able to characterize 18 expanded Combined DNA Index System STRs (D3S1358, D5S818, D2S1338, TPOX, CSF1PO, TH01, vWA, D7S820, D21S11, D10S1248, D8S1179, D1S1656, D18S51, D12S391, D19S433, D16S539, D13S317, and FGA), three highly polymorphic STRs among Chinese people (Penta D, Penta E, and D6S1043), one Y-chromosome Indel and amelogenin using a multiplex PCR; the PCR amplified products were analyzed using the Applied Biosystems 3500 Genetic Analyzer. Full genotyping profiles were obtained using only 31.25 pg of control DNA; various case-type specimens, as well as ten-year-old samples were also successfully genotyped. Additionally, in the validation studies, this multiplex was demonstrated to be human-specific and compatible with the interference of multiple PCR inhibitors. The system also enabled the detection of mixtures, and complete profiles could be obtained at the mixed ratios of 1:1, 1:3, and 3:1. The development and validation study here illustrated that the SiFaSTR™ 23-plex system is accurate, powerful, and more sensitive than many other systems. What's more, the population data in our study not only illustrated that this 23-plex typing system was straightforward and efficient but also expanded the Chinese STR database, which could facilitate the appropriate application of the 23 genetic markers in forensic genetics, especially in the Chinese population.

Authors+Show Affiliations

Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Shanghai, P. R. China.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Shanghai, P. R. China. Institute of Forensic Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, P. R. China.Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Shanghai, P. R. China.Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Shanghai, P. R. China.Institute of Forensic Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, P. R. China.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Shanghai, P. R. China. Department of Forensic Medicine, School of Basic Medical Science, Wenzhou Medical University, Wenzhou, P. R. China.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Shanghai, P. R. China. Department of Forensic Medicine, School of Basic Medical Science, Wenzhou Medical University, Wenzhou, P. R. China.Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Shanghai, P. R. China.Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Shanghai, P. R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31209888

Citation

Zheng, Hancheng, et al. "Development and Validation of a Novel SiFaSTRTM 23-plex System." Electrophoresis, vol. 40, no. 20, 2019, pp. 2644-2654.
Zheng H, Tao R, Zhang J, et al. Development and validation of a novel SiFaSTRTM 23-plex system. Electrophoresis. 2019;40(20):2644-2654.
Zheng, H., Tao, R., Zhang, J., Zhang, J., Wang, S., Yang, Z., Xu, Q., Gao, Y., Zhang, S., & Li, C. (2019). Development and validation of a novel SiFaSTRTM 23-plex system. Electrophoresis, 40(20), 2644-2654. https://doi.org/10.1002/elps.201900045
Zheng H, et al. Development and Validation of a Novel SiFaSTRTM 23-plex System. Electrophoresis. 2019;40(20):2644-2654. PubMed PMID: 31209888.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of a novel SiFaSTRTM 23-plex system. AU - Zheng,Hancheng, AU - Tao,Ruiyang, AU - Zhang,Jingyi, AU - Zhang,Jiashuo, AU - Wang,Shouyu, AU - Yang,Zihao, AU - Xu,Qiannan, AU - Gao,Yuzhen, AU - Zhang,Suhua, AU - Li,Chengtao, Y1 - 2019/06/26/ PY - 2018/12/18/received PY - 2019/03/22/revised PY - 2019/05/23/accepted PY - 2019/6/19/pubmed PY - 2020/4/2/medline PY - 2019/6/19/entrez KW - 23-plex multiplex KW - Capillary electrophoresis KW - DNA genotyping KW - Developmental validation KW - Forensic science SP - 2644 EP - 2654 JF - Electrophoresis JO - Electrophoresis VL - 40 IS - 20 N2 - Human identification and paternity testing are usually based on the study of STRs depending on their particular characteristics in the forensic investigation. In this paper, we developed a sensitive genotyping system, SiFaSTR™ 23-plex, which is able to characterize 18 expanded Combined DNA Index System STRs (D3S1358, D5S818, D2S1338, TPOX, CSF1PO, TH01, vWA, D7S820, D21S11, D10S1248, D8S1179, D1S1656, D18S51, D12S391, D19S433, D16S539, D13S317, and FGA), three highly polymorphic STRs among Chinese people (Penta D, Penta E, and D6S1043), one Y-chromosome Indel and amelogenin using a multiplex PCR; the PCR amplified products were analyzed using the Applied Biosystems 3500 Genetic Analyzer. Full genotyping profiles were obtained using only 31.25 pg of control DNA; various case-type specimens, as well as ten-year-old samples were also successfully genotyped. Additionally, in the validation studies, this multiplex was demonstrated to be human-specific and compatible with the interference of multiple PCR inhibitors. The system also enabled the detection of mixtures, and complete profiles could be obtained at the mixed ratios of 1:1, 1:3, and 3:1. The development and validation study here illustrated that the SiFaSTR™ 23-plex system is accurate, powerful, and more sensitive than many other systems. What's more, the population data in our study not only illustrated that this 23-plex typing system was straightforward and efficient but also expanded the Chinese STR database, which could facilitate the appropriate application of the 23 genetic markers in forensic genetics, especially in the Chinese population. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/31209888/Development_and_validation_of_a_novel_SiFaSTRTM_23_plex_system_ L2 - https://doi.org/10.1002/elps.201900045 DB - PRIME DP - Unbound Medicine ER -