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Three-finger toxins from the venom of Micrurus tschudii tschudii (desert coral snake): Isolation and characterization of tschuditoxin-I.
Toxicon. 2019 Sep; 167:144-151.T

Abstract

Venoms from Micrurus (New World coral snakes) display potent peripheral neurotoxicity which may cause death by respiratory paralysis, yet many are poorly or not characterized. The major venom components of coral snakes are three-finger toxins (3FTxs) and phospholipases A2, whose proportions vary among species. As a trend, venoms of Micrurus from South America contain high proportions of 3FTxs, in contrast to most North and Central American species. Micrurus tschudii tschudii, the 'Desert coral snake' from Perú, displays an extreme 3FTx-predominant venom phenotype, with ∼95% of its proteome belonging to this protein family. This study evaluated the toxicity of its major 3FTxs in mice. A lethal 3FTx, here named tschuditoxin-I, was purified and sequenced by MALDI-TOF-TOF and N-terminal degradation. Tschuditoxin-I showed highest similarity to MS-1, a short-chain α-neurotoxin from the aquatic, fish-eating coral snake M. surinamensis. The single amino acid substitution between these two toxins maps at the tip of the first β-stranded 'finger' in the modeled structure of tschuditoxin-I, suggesting it may have a role in interaction with its target, which remains to be investigated. Owing to its lethal action, tschuditoxin-I is likely to be medically relevant in envenomings. In spite of its 74% sequence identity with an α-neurotoxin of M. nigrocinctus, an equine antivenom raised against venom of the latter did not immunorecognize tschuditoxin-I or M. t. tschudii venom by ELISA. This underscores the need of characterizing the biochemical and immunological properties of the main toxic components of Micrurus venoms, aiming to improve the limited para-specific coverage of current antivenoms.

Authors+Show Affiliations

Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, 11501, Costa Rica. Electronic address: bruno.lomonte@ucr.ac.cr.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, 11501, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, 11501, Costa Rica.Departamento Académico de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima 31, Peru.Departamento Académico de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima 31, Peru.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31211957

Citation

Lomonte, Bruno, et al. "Three-finger Toxins From the Venom of Micrurus Tschudii Tschudii (desert Coral Snake): Isolation and Characterization of Tschuditoxin-I." Toxicon : Official Journal of the International Society On Toxinology, vol. 167, 2019, pp. 144-151.
Lomonte B, Camacho E, Fernández J, et al. Three-finger toxins from the venom of Micrurus tschudii tschudii (desert coral snake): Isolation and characterization of tschuditoxin-I. Toxicon. 2019;167:144-151.
Lomonte, B., Camacho, E., Fernández, J., Salas, M., & Zavaleta, A. (2019). Three-finger toxins from the venom of Micrurus tschudii tschudii (desert coral snake): Isolation and characterization of tschuditoxin-I. Toxicon : Official Journal of the International Society On Toxinology, 167, 144-151. https://doi.org/10.1016/j.toxicon.2019.06.023
Lomonte B, et al. Three-finger Toxins From the Venom of Micrurus Tschudii Tschudii (desert Coral Snake): Isolation and Characterization of Tschuditoxin-I. Toxicon. 2019;167:144-151. PubMed PMID: 31211957.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Three-finger toxins from the venom of Micrurus tschudii tschudii (desert coral snake): Isolation and characterization of tschuditoxin-I. AU - Lomonte,Bruno, AU - Camacho,Erika, AU - Fernández,Julián, AU - Salas,María, AU - Zavaleta,Alfonso, Y1 - 2019/06/15/ PY - 2019/03/01/received PY - 2019/06/08/revised PY - 2019/06/14/accepted PY - 2019/6/19/pubmed PY - 2020/3/11/medline PY - 2019/6/19/entrez KW - Coral snake KW - Micrurus t. tschudii KW - Snake venom KW - Three-finger toxins SP - 144 EP - 151 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 167 N2 - Venoms from Micrurus (New World coral snakes) display potent peripheral neurotoxicity which may cause death by respiratory paralysis, yet many are poorly or not characterized. The major venom components of coral snakes are three-finger toxins (3FTxs) and phospholipases A2, whose proportions vary among species. As a trend, venoms of Micrurus from South America contain high proportions of 3FTxs, in contrast to most North and Central American species. Micrurus tschudii tschudii, the 'Desert coral snake' from Perú, displays an extreme 3FTx-predominant venom phenotype, with ∼95% of its proteome belonging to this protein family. This study evaluated the toxicity of its major 3FTxs in mice. A lethal 3FTx, here named tschuditoxin-I, was purified and sequenced by MALDI-TOF-TOF and N-terminal degradation. Tschuditoxin-I showed highest similarity to MS-1, a short-chain α-neurotoxin from the aquatic, fish-eating coral snake M. surinamensis. The single amino acid substitution between these two toxins maps at the tip of the first β-stranded 'finger' in the modeled structure of tschuditoxin-I, suggesting it may have a role in interaction with its target, which remains to be investigated. Owing to its lethal action, tschuditoxin-I is likely to be medically relevant in envenomings. In spite of its 74% sequence identity with an α-neurotoxin of M. nigrocinctus, an equine antivenom raised against venom of the latter did not immunorecognize tschuditoxin-I or M. t. tschudii venom by ELISA. This underscores the need of characterizing the biochemical and immunological properties of the main toxic components of Micrurus venoms, aiming to improve the limited para-specific coverage of current antivenoms. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/31211957/Three_finger_toxins_from_the_venom_of_Micrurus_tschudii_tschudii__desert_coral_snake_:_Isolation_and_characterization_of_tschuditoxin_I_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(19)30198-9 DB - PRIME DP - Unbound Medicine ER -