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Development of American-Lineage Influenza H5N2 Reassortant Vaccine Viruses for Pandemic Preparedness.
Viruses. 2019 06 11; 11(6)V

Abstract

Novel low-pathogenic avian influenza (LPAI) H5N2 viruses hit poultry farms in Taiwan in 2003, and evolved into highly pathogenic avian influenza (HPAI) viruses in 2010. These viruses are reassortant viruses containing HA and NA genes from American-lineage H5N2 and six internal genes from local H6N1 viruses. According to a serological survey, the Taiwan H5N2 viruses can cause asymptomatic infections in poultry workers. Therefore, a development of influenza H5N2 vaccines is desirable for pandemic preparation. In this study, we employed reverse genetics to generate a vaccine virus having HA and NA genes from A/Chicken/CY/A2628/2012 (E7, LPAI) and six internal genes from a Vero cell-adapted high-growth H5N1 vaccine virus (Vero-15). The reassortant H5N2 vaccine virus, E7-V15, presented high-growth efficiency in Vero cells (512 HAU, 107.6 TCID50/mL), and passed all tests for qualification of candidate vaccine viruses. In ferret immunization, two doses of inactivated whole virus antigens (3 μg of HA protein) adjuvanted with alum could induce robust antibody response (HI titre 113.14). In conclusion, we have established reverse genetics to generate a qualified reassortant H5N2 vaccine virus for further development.

Authors+Show Affiliations

National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. letitia@nhri.org.tw. Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 30013, Taiwan. letitia@nhri.org.tw.National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. alanhu@nhri.org.tw.National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. grayingaries@outlook.com.National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. wtc@nhri.org.tw.National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. laicc2@nhri.org.tw. College of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan. laicc2@nhri.org.tw.National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. yufents@gmail.com.Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan. mccheng@mail.npust.edu.tw. Animal Health Research Institutes, Danshui, New Taipei City 25158, Taiwan. mccheng@mail.npust.edu.tw.National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. chiaminyuan@dragon.nchu.edu.tw. Department of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan. chiaminyuan@dragon.nchu.edu.tw.Institute of Preventive Medicine, National Defence Medical Centre, Taipei 23742, Taiwan. spps057@gmail.com.Institute of Preventive Medicine, National Defence Medical Centre, Taipei 23742, Taiwan. yyhome@mail.ndmctsgh.edu.tw.National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. suihjen0704@stust.edu.tw.National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. minshi@nhri.org.tw.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31212631

Citation

Chen, Po-Ling, et al. "Development of American-Lineage Influenza H5N2 Reassortant Vaccine Viruses for Pandemic Preparedness." Viruses, vol. 11, no. 6, 2019.
Chen PL, Hu AY, Lin CY, et al. Development of American-Lineage Influenza H5N2 Reassortant Vaccine Viruses for Pandemic Preparedness. Viruses. 2019;11(6).
Chen, P. L., Hu, A. Y., Lin, C. Y., Weng, T. C., Lai, C. C., Tseng, Y. F., Cheng, M. C., Chia, M. Y., Lin, W. C., Yeh, C. T., Su, I. J., & Lee, M. S. (2019). Development of American-Lineage Influenza H5N2 Reassortant Vaccine Viruses for Pandemic Preparedness. Viruses, 11(6). https://doi.org/10.3390/v11060543
Chen PL, et al. Development of American-Lineage Influenza H5N2 Reassortant Vaccine Viruses for Pandemic Preparedness. Viruses. 2019 06 11;11(6) PubMed PMID: 31212631.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of American-Lineage Influenza H5N2 Reassortant Vaccine Viruses for Pandemic Preparedness. AU - Chen,Po-Ling, AU - Hu,Alan Yung-Chih, AU - Lin,Chun-Yang, AU - Weng,Tsai-Chuan, AU - Lai,Chia-Chun, AU - Tseng,Yu-Fen, AU - Cheng,Ming-Chu, AU - Chia,Min-Yuan, AU - Lin,Wen-Chin, AU - Yeh,Chia-Tsui, AU - Su,Ih-Jen, AU - Lee,Min-Shi, Y1 - 2019/06/11/ PY - 2019/04/23/received PY - 2019/06/04/revised PY - 2019/06/05/accepted PY - 2019/6/20/entrez PY - 2019/6/20/pubmed PY - 2020/7/25/medline KW - American-lineage H5N2 vaccine KW - American-lineage reassortant influenza viruses KW - Pandemic preparedness JF - Viruses JO - Viruses VL - 11 IS - 6 N2 - Novel low-pathogenic avian influenza (LPAI) H5N2 viruses hit poultry farms in Taiwan in 2003, and evolved into highly pathogenic avian influenza (HPAI) viruses in 2010. These viruses are reassortant viruses containing HA and NA genes from American-lineage H5N2 and six internal genes from local H6N1 viruses. According to a serological survey, the Taiwan H5N2 viruses can cause asymptomatic infections in poultry workers. Therefore, a development of influenza H5N2 vaccines is desirable for pandemic preparation. In this study, we employed reverse genetics to generate a vaccine virus having HA and NA genes from A/Chicken/CY/A2628/2012 (E7, LPAI) and six internal genes from a Vero cell-adapted high-growth H5N1 vaccine virus (Vero-15). The reassortant H5N2 vaccine virus, E7-V15, presented high-growth efficiency in Vero cells (512 HAU, 107.6 TCID50/mL), and passed all tests for qualification of candidate vaccine viruses. In ferret immunization, two doses of inactivated whole virus antigens (3 μg of HA protein) adjuvanted with alum could induce robust antibody response (HI titre 113.14). In conclusion, we have established reverse genetics to generate a qualified reassortant H5N2 vaccine virus for further development. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/31212631/Development_of_American_Lineage_Influenza_H5N2_Reassortant_Vaccine_Viruses_for_Pandemic_Preparedness_ L2 - https://www.mdpi.com/resolver?pii=v11060543 DB - PRIME DP - Unbound Medicine ER -