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Diversification of the downy mildew resistance gene pool by introgression of a new gene, Pl35, from wild Helianthus argophyllus into oilseed and confection sunflowers (Helianthus annuus L.).
Theor Appl Genet. 2019 Sep; 132(9):2553-2565.TA

Abstract

KEY MESSAGE

We have mapped a new downy mildew resistance gene, Pl35, derived from wild Helianthus argophyllus to sunflower linkage group 1. New germplasms incorporating the Pl35 gene were developed for both oilseed and confection sunflower Sunflower downy mildew (DM), caused by the oomycete pathogen Plasmopara halstedii, is an economically important and widespread sunflower disease worldwide. Non-race-specific resistance is not available in sunflower, and breeding for DM resistance relies on race-specific resistance to control this disease. The discovery of the novel DM resistance genes is a long-term task due to the highly virulent and aggressive nature of the P. halstedii pathogen, which reduces the effectiveness of resistance genes. The objectives of this study were to: (1) transfer DM resistance from a wild sunflower species Helianthus argophyllus (PI 494576) into cultivated sunflowers; (2) map the resistance gene; and (3) develop diagnostic single-nucleotide polymorphism (SNP) markers for efficient targeting of the gene in breeding programs. The H. argophyllus accession PI 494576 previously identified with resistance to the most virulent P. halstedii race 777 was crossed with oilseed and confection sunflower in 2012. Molecular mapping using the BC2F2 and BC2F3 populations derived from the cross CONFSCLB1/PI 494576 located a new resistance gene Pl35 on linkage group 1 of the sunflower genome. The new gene Pl35 was successfully transferred from PI 494576 into cultivated sunflowers. SNP markers flanking Pl35 were surveyed in a validation panel of 548 diversified sunflower lines collected globally. Eleven SNP markers were found to be diagnostic for Pl35 SNP alleles, with four co-segregating with Pl35. The developed oilseed and confection germplasms with diagnostic SNP markers for Pl35 will be very useful resources for breeding of DM resistance in sunflower.

Authors+Show Affiliations

USDA-Agricultural Research Service, Edward T. Schafer Agricultural Research Center, 1616 Albrecht Blvd. N, Fargo, ND, 58102-2765, USA. lili.qi@ars.usda.gov.Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA.Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA.USDA-Agricultural Research Service, Edward T. Schafer Agricultural Research Center, 1616 Albrecht Blvd. N, Fargo, ND, 58102-2765, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31214741

Citation

Qi, L L., et al. "Diversification of the Downy Mildew Resistance Gene Pool By Introgression of a New Gene, Pl35, From Wild Helianthus Argophyllus Into Oilseed and Confection Sunflowers (Helianthus Annuus L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 132, no. 9, 2019, pp. 2553-2565.
Qi LL, Ma GJ, Li XH, et al. Diversification of the downy mildew resistance gene pool by introgression of a new gene, Pl35, from wild Helianthus argophyllus into oilseed and confection sunflowers (Helianthus annuus L.). Theor Appl Genet. 2019;132(9):2553-2565.
Qi, L. L., Ma, G. J., Li, X. H., & Seiler, G. J. (2019). Diversification of the downy mildew resistance gene pool by introgression of a new gene, Pl35, from wild Helianthus argophyllus into oilseed and confection sunflowers (Helianthus annuus L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 132(9), 2553-2565. https://doi.org/10.1007/s00122-019-03370-9
Qi LL, et al. Diversification of the Downy Mildew Resistance Gene Pool By Introgression of a New Gene, Pl35, From Wild Helianthus Argophyllus Into Oilseed and Confection Sunflowers (Helianthus Annuus L.). Theor Appl Genet. 2019;132(9):2553-2565. PubMed PMID: 31214741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversification of the downy mildew resistance gene pool by introgression of a new gene, Pl35, from wild Helianthus argophyllus into oilseed and confection sunflowers (Helianthus annuus L.). AU - Qi,L L, AU - Ma,G J, AU - Li,X H, AU - Seiler,G J, Y1 - 2019/06/18/ PY - 2019/03/12/received PY - 2019/06/03/accepted PY - 2019/6/20/pubmed PY - 2020/2/11/medline PY - 2019/6/20/entrez SP - 2553 EP - 2565 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 132 IS - 9 N2 - KEY MESSAGE: We have mapped a new downy mildew resistance gene, Pl35, derived from wild Helianthus argophyllus to sunflower linkage group 1. New germplasms incorporating the Pl35 gene were developed for both oilseed and confection sunflower Sunflower downy mildew (DM), caused by the oomycete pathogen Plasmopara halstedii, is an economically important and widespread sunflower disease worldwide. Non-race-specific resistance is not available in sunflower, and breeding for DM resistance relies on race-specific resistance to control this disease. The discovery of the novel DM resistance genes is a long-term task due to the highly virulent and aggressive nature of the P. halstedii pathogen, which reduces the effectiveness of resistance genes. The objectives of this study were to: (1) transfer DM resistance from a wild sunflower species Helianthus argophyllus (PI 494576) into cultivated sunflowers; (2) map the resistance gene; and (3) develop diagnostic single-nucleotide polymorphism (SNP) markers for efficient targeting of the gene in breeding programs. The H. argophyllus accession PI 494576 previously identified with resistance to the most virulent P. halstedii race 777 was crossed with oilseed and confection sunflower in 2012. Molecular mapping using the BC2F2 and BC2F3 populations derived from the cross CONFSCLB1/PI 494576 located a new resistance gene Pl35 on linkage group 1 of the sunflower genome. The new gene Pl35 was successfully transferred from PI 494576 into cultivated sunflowers. SNP markers flanking Pl35 were surveyed in a validation panel of 548 diversified sunflower lines collected globally. Eleven SNP markers were found to be diagnostic for Pl35 SNP alleles, with four co-segregating with Pl35. The developed oilseed and confection germplasms with diagnostic SNP markers for Pl35 will be very useful resources for breeding of DM resistance in sunflower. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/31214741/Diversification_of_the_downy_mildew_resistance_gene_pool_by_introgression_of_a_new_gene_Pl35_from_wild_Helianthus_argophyllus_into_oilseed_and_confection_sunflowers__Helianthus_annuus_L___ L2 - https://dx.doi.org/10.1007/s00122-019-03370-9 DB - PRIME DP - Unbound Medicine ER -