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Probing the Interaction of Newly Synthesized Pt(II) Complex on Human Serum Albumin Using Competitive Binding Site Markers.

Abstract

Considering the importance of pharmacology and the influence of drugs on biological materials, the effects of a newly designed and synthesized platin complex (2,2'-Bipyridine-3,3'-dicarboxylic acid, oxalato Platinum(II), as an antitumor drug was tested on the structure of blood carrier protein of human serum albumin (HSA) using various spectroscopic techniques including UV-visible, fluorescence, and circular dichroism at 25 and 37 °C. Results of the fluorescence measurements revealed that adding the complex caused reduction in intrinsic fluorescence emission of HSA resulted from dynamic quenching of HSA. The number of binding sites and binding constants were calculated at both temperatures of 25 and 37 °C. In addition, in order to identify the complex's binding site on HSA employing spectroscopy, the competitive studies were followed using warfarin, digitoxin and ibuprofen as site markers of Sudlow sites I, II and III. Competitive binding test results have shown that Pt(II) complex bind on the warfarin binding site (or Sudlow sites I) on HSA. Besides, a reduction in thermal stability for HSA was observed in the presence of the newly designed Pt(II) complex.

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  • Authors+Show Affiliations

    ,

    Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

    ,

    Department of Cell & Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran. divsalar@khu.ac.ir.

    ,

    Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.

    Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

    Source

    Journal of fluorescence : 2019 Jun 19 pg

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31218597

    Citation

    Najaran, Akram, et al. "Probing the Interaction of Newly Synthesized Pt(II) Complex On Human Serum Albumin Using Competitive Binding Site Markers." Journal of Fluorescence, 2019.
    Najaran A, Divsalar A, Saboury AA, et al. Probing the Interaction of Newly Synthesized Pt(II) Complex on Human Serum Albumin Using Competitive Binding Site Markers. J Fluoresc. 2019.
    Najaran, A., Divsalar, A., Saboury, A. A., & Roodbari, N. H. (2019). Probing the Interaction of Newly Synthesized Pt(II) Complex on Human Serum Albumin Using Competitive Binding Site Markers. Journal of Fluorescence, doi:10.1007/s10895-019-02383-3.
    Najaran A, et al. Probing the Interaction of Newly Synthesized Pt(II) Complex On Human Serum Albumin Using Competitive Binding Site Markers. J Fluoresc. 2019 Jun 19; PubMed PMID: 31218597.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Probing the Interaction of Newly Synthesized Pt(II) Complex on Human Serum Albumin Using Competitive Binding Site Markers. AU - Najaran,Akram, AU - Divsalar,Adeleh, AU - Saboury,Ali Akbar, AU - Roodbari,Nasim Hayati, Y1 - 2019/06/19/ PY - 2019/02/06/received PY - 2019/04/17/accepted PY - 2019/6/21/entrez KW - Competitive studies KW - Fluorescence quenching KW - HSA KW - Site markers KW - Sudlow sites JF - Journal of fluorescence JO - J Fluoresc N2 - Considering the importance of pharmacology and the influence of drugs on biological materials, the effects of a newly designed and synthesized platin complex (2,2'-Bipyridine-3,3'-dicarboxylic acid, oxalato Platinum(II), as an antitumor drug was tested on the structure of blood carrier protein of human serum albumin (HSA) using various spectroscopic techniques including UV-visible, fluorescence, and circular dichroism at 25 and 37 °C. Results of the fluorescence measurements revealed that adding the complex caused reduction in intrinsic fluorescence emission of HSA resulted from dynamic quenching of HSA. The number of binding sites and binding constants were calculated at both temperatures of 25 and 37 °C. In addition, in order to identify the complex's binding site on HSA employing spectroscopy, the competitive studies were followed using warfarin, digitoxin and ibuprofen as site markers of Sudlow sites I, II and III. Competitive binding test results have shown that Pt(II) complex bind on the warfarin binding site (or Sudlow sites I) on HSA. Besides, a reduction in thermal stability for HSA was observed in the presence of the newly designed Pt(II) complex. SN - 1573-4994 UR - https://www.unboundmedicine.com/medline/citation/31218597/Probing_the_Interaction_of_Newly_Synthesized_Pt(II)_Complex_on_Human_Serum_Albumin_Using_Competitive_Binding_Site_Markers L2 - https://doi.org/10.1007/s10895-019-02383-3 DB - PRIME DP - Unbound Medicine ER -