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Naloxone prolongs abdominal constriction writhing-like behavior in a zebrafish-based pain model.
Neurosci Lett 2019; 708:134336NL

Abstract

The ability to detect noxious stimuli is essential to survival. However, pathological pain is maladaptive and severely debilitating. Endogenous and exogenous opioids modulate pain responses via opioid receptors, reducing pain sensibility. Due to the high genetic and physiological similarities to rodents and humans, the zebrafish is a valuable tool to assess pain responses and the underlying mechanisms involved in nociception. Although morphine attenuates pain-like responses of zebrafish, there are no data showing if the antagonism of opioid receptors prolongs pain duration in the absence of an exogenous opioid. Here, we investigated whether a common opioid antagonist naloxone affects the abdominal constriction writhing-like response, recently characterized as a zebrafish-based pain behavior. Animals were injected intraperitoneally with acetic acid (5.0%), naloxone (1.25 mg/kg; 2.5 mg/kg; 5.0 mg/kg) or acetic acid with naloxone to investigate the changes in their body curvature for 1 h. Acetic acid elicited a robust pain-like response in zebrafish, as assessed by aberrant abdominal body curvature, while no effects were observed following PBS injection. Although naloxone alone did not alter the frequency and duration of this behavior, it dose-dependently prolonged acetic acid-induced abdominal curvature response. Besides reinforcing the use of the abdominal writhing-like phenotype as a behavioral endpoint to measure acute pain responses in zebrafish models, our novel data suggest a putative role of endogenous opioids in modulating the recovery from pain stimulation in zebrafish.

Authors+Show Affiliations

Laboratory of Experimental Neuropsychobiology, Department of Biochemistry and Molecular Biology, Natural and Exact Sciences Center, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil; Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria. 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil. Electronic address: fvcosta88@gmail.com.Laboratory of Experimental Neuropsychobiology, Department of Biochemistry and Molecular Biology, Natural and Exact Sciences Center, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil; Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria. 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.Laboratory of Experimental Neuropsychobiology, Department of Biochemistry and Molecular Biology, Natural and Exact Sciences Center, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.School of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China; Institute of Experimental Medicine, Almazov National Medical Research Center, St. Petersburg 197341, Russia; Ural Federal University, 19 Mira street, Ekaterinburg, 620002, Russia; The International Zebrafish Neuroscience Research Consortium (ZNRC), 309 Palmer Court, Slidell, LA 70458, USA.Laboratory of Experimental Neuropsychobiology, Department of Biochemistry and Molecular Biology, Natural and Exact Sciences Center, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil; Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria. 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil; The International Zebrafish Neuroscience Research Consortium (ZNRC), 309 Palmer Court, Slidell, LA 70458, USA. Electronic address: dbrosemberg@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31220523

Citation

Costa, Fabiano V., et al. "Naloxone Prolongs Abdominal Constriction Writhing-like Behavior in a Zebrafish-based Pain Model." Neuroscience Letters, vol. 708, 2019, p. 134336.
Costa FV, Canzian J, Stefanello FV, et al. Naloxone prolongs abdominal constriction writhing-like behavior in a zebrafish-based pain model. Neurosci Lett. 2019;708:134336.
Costa, F. V., Canzian, J., Stefanello, F. V., Kalueff, A. V., & Rosemberg, D. B. (2019). Naloxone prolongs abdominal constriction writhing-like behavior in a zebrafish-based pain model. Neuroscience Letters, 708, p. 134336. doi:10.1016/j.neulet.2019.134336.
Costa FV, et al. Naloxone Prolongs Abdominal Constriction Writhing-like Behavior in a Zebrafish-based Pain Model. Neurosci Lett. 2019 08 24;708:134336. PubMed PMID: 31220523.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Naloxone prolongs abdominal constriction writhing-like behavior in a zebrafish-based pain model. AU - Costa,Fabiano V, AU - Canzian,Julia, AU - Stefanello,Flavia V, AU - Kalueff,Allan V, AU - Rosemberg,Denis B, Y1 - 2019/06/18/ PY - 2019/05/06/received PY - 2019/06/07/revised PY - 2019/06/16/accepted PY - 2019/6/21/pubmed PY - 2019/6/21/medline PY - 2019/6/21/entrez KW - Abdominal constriction writhing-like behavior KW - Acetic acid KW - Naloxone KW - Nociception KW - Zebrafish SP - 134336 EP - 134336 JF - Neuroscience letters JO - Neurosci. Lett. VL - 708 N2 - The ability to detect noxious stimuli is essential to survival. However, pathological pain is maladaptive and severely debilitating. Endogenous and exogenous opioids modulate pain responses via opioid receptors, reducing pain sensibility. Due to the high genetic and physiological similarities to rodents and humans, the zebrafish is a valuable tool to assess pain responses and the underlying mechanisms involved in nociception. Although morphine attenuates pain-like responses of zebrafish, there are no data showing if the antagonism of opioid receptors prolongs pain duration in the absence of an exogenous opioid. Here, we investigated whether a common opioid antagonist naloxone affects the abdominal constriction writhing-like response, recently characterized as a zebrafish-based pain behavior. Animals were injected intraperitoneally with acetic acid (5.0%), naloxone (1.25 mg/kg; 2.5 mg/kg; 5.0 mg/kg) or acetic acid with naloxone to investigate the changes in their body curvature for 1 h. Acetic acid elicited a robust pain-like response in zebrafish, as assessed by aberrant abdominal body curvature, while no effects were observed following PBS injection. Although naloxone alone did not alter the frequency and duration of this behavior, it dose-dependently prolonged acetic acid-induced abdominal curvature response. Besides reinforcing the use of the abdominal writhing-like phenotype as a behavioral endpoint to measure acute pain responses in zebrafish models, our novel data suggest a putative role of endogenous opioids in modulating the recovery from pain stimulation in zebrafish. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/31220523/Naloxone_prolongs_abdominal_constriction_writhing-like_behavior_in_a_zebrafish-based_pain_model L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(19)30435-5 DB - PRIME DP - Unbound Medicine ER -