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Chemical structure and snake antivenom properties of sulfated agarans obtained from Laurencia dendroidea (Ceramiales, Rhodophyta).
Carbohydr Polym. 2019 Aug 15; 218:136-144.CP

Abstract

Aqueous and KCl-soluble polysaccharides were extracted from Laurencia dendroidea (Rhodomelaceae, Ceramiales) and their chemical profile was accessed by anion-exchange chromatography, chemical and spectroscopic analyses. The homogeneous agaran DHS-4 (181.3 × 103 g. mol-1, 21.3% of NaSO3) presents A units mostly 2-sulfated (18.9 mol%), nonsubstituted (15.3 mol%) and 6-O-methylated (10.1 mol%), while B units are l-sugars composed predominantly by galactose 6-sulfate precursor units (19.2 mol%) and 3,6-anhydrogalactose (13.8 mol%), besides non-precursor galactose 6-sulfate units bearing d-xylose substituents on C-3 (8.1 mol%). The crude KCl-soluble DHS agaran (20.5% of NaSO3) inhibited proteolysis and hemolysis induced by Lachesis muta and Bothrops jararaca venoms. DHS was able to inhibit up to 75% the L. muta venom hemorrhagic effect and to reduce the lethality displayed by B. jararaca venom, increasing the mice survival time up to 3 times. Therefore, this agaran has high potential to be used as an additional tool to treat snakebite envenomation.

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Authors+Show Affiliations

Ferreira LG
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, Brazil. Electronic address: luciana.g.ferreira@tjpr.jus.br.
da Silva ACR
Departamento de Biologia Celular e Molecular, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil. Electronic address: anacrs1@yahoo.com.br.
Noseda MD
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, Brazil. Electronic address: mdn@ufpr.br.
Fuly AL
Departamento de Biologia Celular e Molecular, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil. Electronic address: andrefuly@id.uff.br.
de Carvalho MM
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, Brazil. Electronic address: mariana.mazetto@ufpr.br.
Fujii MT
Instituto de Botânica, São Paulo, São Paulo, Brazil. Electronic address: mutue.fujii@gmail.com.
Sanchez EF
Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil. Electronic address: eladiooswaldo@gmail.com.
Carneiro J
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, Brazil. Electronic address: jaquelinecarneiro@ufpr.br.
Duarte MER
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, Brazil. Electronic address: eugenia.duarte@pq.cnpq.br.

MeSH

AnimalsBothropsHemolysin ProteinsHemolysisHemostaticsLaurenciaMicePolysaccharidesProteolysisSnake VenomsSulfuric Acid EstersViperidae

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31221314

Citation

Ferreira, Luciana G., et al. "Chemical Structure and Snake Antivenom Properties of Sulfated Agarans Obtained From Laurencia Dendroidea (Ceramiales, Rhodophyta)." Carbohydrate Polymers, vol. 218, 2019, pp. 136-144.
Ferreira LG, da Silva ACR, Noseda MD, et al. Chemical structure and snake antivenom properties of sulfated agarans obtained from Laurencia dendroidea (Ceramiales, Rhodophyta). Carbohydr Polym. 2019;218:136-144.
Ferreira, L. G., da Silva, A. C. R., Noseda, M. D., Fuly, A. L., de Carvalho, M. M., Fujii, M. T., Sanchez, E. F., Carneiro, J., & Duarte, M. E. R. (2019). Chemical structure and snake antivenom properties of sulfated agarans obtained from Laurencia dendroidea (Ceramiales, Rhodophyta). Carbohydrate Polymers, 218, 136-144. https://doi.org/10.1016/j.carbpol.2019.04.066
Ferreira LG, et al. Chemical Structure and Snake Antivenom Properties of Sulfated Agarans Obtained From Laurencia Dendroidea (Ceramiales, Rhodophyta). Carbohydr Polym. 2019 Aug 15;218:136-144. PubMed PMID: 31221314.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical structure and snake antivenom properties of sulfated agarans obtained from Laurencia dendroidea (Ceramiales, Rhodophyta). AU - Ferreira,Luciana G, AU - da Silva,Ana Cláudia R, AU - Noseda,Miguel D, AU - Fuly,André L, AU - de Carvalho,Mariana M, AU - Fujii,Mutue T, AU - Sanchez,Eladio F, AU - Carneiro,Jaqueline, AU - Duarte,Maria Eugênia R, Y1 - 2019/04/30/ PY - 2019/02/18/received PY - 2019/04/18/revised PY - 2019/04/18/accepted PY - 2019/6/22/entrez PY - 2019/6/22/pubmed PY - 2019/12/4/medline KW - Antivenom activity KW - Bothrops jararaca KW - Chemical structure KW - Lachesis muta KW - Red seaweed KW - Sulfated agaran SP - 136 EP - 144 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 218 N2 - Aqueous and KCl-soluble polysaccharides were extracted from Laurencia dendroidea (Rhodomelaceae, Ceramiales) and their chemical profile was accessed by anion-exchange chromatography, chemical and spectroscopic analyses. The homogeneous agaran DHS-4 (181.3 × 103 g. mol-1, 21.3% of NaSO3) presents A units mostly 2-sulfated (18.9 mol%), nonsubstituted (15.3 mol%) and 6-O-methylated (10.1 mol%), while B units are l-sugars composed predominantly by galactose 6-sulfate precursor units (19.2 mol%) and 3,6-anhydrogalactose (13.8 mol%), besides non-precursor galactose 6-sulfate units bearing d-xylose substituents on C-3 (8.1 mol%). The crude KCl-soluble DHS agaran (20.5% of NaSO3) inhibited proteolysis and hemolysis induced by Lachesis muta and Bothrops jararaca venoms. DHS was able to inhibit up to 75% the L. muta venom hemorrhagic effect and to reduce the lethality displayed by B. jararaca venom, increasing the mice survival time up to 3 times. Therefore, this agaran has high potential to be used as an additional tool to treat snakebite envenomation. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/31221314/Chemical_structure_and_snake_antivenom_properties_of_sulfated_agarans_obtained_from_Laurencia_dendroidea__Ceramiales_Rhodophyta__ DB - PRIME DP - Unbound Medicine ER -