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Cell migration directionality and speed are independently regulated by RasG and Gβ in Dictyostelium cells in electrotaxis.
Biol Open 2019; 8(7)BO

Abstract

Motile cells manifest increased migration speed and directionality in gradients of stimuli, including chemoattractants, electrical potential and substratum stiffness. Here, we demonstrate that Dictyostelium cells move directionally in response to an electric field (EF) with specific acceleration/deceleration kinetics of directionality and migration speed. Detailed analyses of the migration kinetics suggest that migration speed and directionality are separately regulated by Gβ and RasG, respectively, in EF-directed cell migration. Cells lacking Gβ, which is essential for all chemotactic responses in Dictyostelium, showed EF-directed cell migration with the same increase in directionality in an EF as wild-type cells. However, these cells failed to show induction of the migration speed upon EF stimulation as much as wild-type cells. Loss of RasG, a key regulator of chemoattractant-directed cell migration, resulted in almost complete loss of directionality, but similar acceleration/deceleration kinetics of migration speed as wild-type cells. These results indicate that Gβ and RasG are required for the induction of migration speed and directionality, respectively, in response to an EF, suggesting separation of migration speed and directionality even with intact feedback loops between mechanical and signaling networks.

Authors+Show Affiliations

Department of Biology & BK21-Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University, Gwangju 61452, Republic of Korea minzhao@ucdavis.edu tjeon@chosun.ac.kr.School of life science, Yunnan Normal University, Kunming, Yunnan 650500, China.Department of Biology & BK21-Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University, Gwangju 61452, Republic of Korea.Department of Biology & BK21-Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University, Gwangju 61452, Republic of Korea.Department of Biology & BK21-Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University, Gwangju 61452, Republic of Korea.Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.Departments of Dermatology and Ophthalmology, Institute for Regenerative Cures, School of Medicine, University of California at Davis, CA 95817, USA minzhao@ucdavis.edu tjeon@chosun.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31221628

Citation

Jeon, Taeck J., et al. "Cell Migration Directionality and Speed Are Independently Regulated By RasG and Gβ in Dictyostelium Cells in Electrotaxis." Biology Open, vol. 8, no. 7, 2019.
Jeon TJ, Gao R, Kim H, et al. Cell migration directionality and speed are independently regulated by RasG and Gβ in Dictyostelium cells in electrotaxis. Biol Open. 2019;8(7).
Jeon, T. J., Gao, R., Kim, H., Lee, A., Jeon, P., Devreotes, P. N., & Zhao, M. (2019). Cell migration directionality and speed are independently regulated by RasG and Gβ in Dictyostelium cells in electrotaxis. Biology Open, 8(7), doi:10.1242/bio.042457.
Jeon TJ, et al. Cell Migration Directionality and Speed Are Independently Regulated By RasG and Gβ in Dictyostelium Cells in Electrotaxis. Biol Open. 2019 Jul 4;8(7) PubMed PMID: 31221628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cell migration directionality and speed are independently regulated by RasG and Gβ in Dictyostelium cells in electrotaxis. AU - Jeon,Taeck J, AU - Gao,Runchi, AU - Kim,Hyeseon, AU - Lee,Ara, AU - Jeon,Pyeonghwa, AU - Devreotes,Peter N, AU - Zhao,Min, Y1 - 2019/07/04/ PY - 2019/6/22/pubmed PY - 2019/6/22/medline PY - 2019/6/22/entrez KW - Dictyostelium KW - Directionality KW - Electrotaxis KW - Migration speed KW - Motility JF - Biology open JO - Biol Open VL - 8 IS - 7 N2 - Motile cells manifest increased migration speed and directionality in gradients of stimuli, including chemoattractants, electrical potential and substratum stiffness. Here, we demonstrate that Dictyostelium cells move directionally in response to an electric field (EF) with specific acceleration/deceleration kinetics of directionality and migration speed. Detailed analyses of the migration kinetics suggest that migration speed and directionality are separately regulated by Gβ and RasG, respectively, in EF-directed cell migration. Cells lacking Gβ, which is essential for all chemotactic responses in Dictyostelium, showed EF-directed cell migration with the same increase in directionality in an EF as wild-type cells. However, these cells failed to show induction of the migration speed upon EF stimulation as much as wild-type cells. Loss of RasG, a key regulator of chemoattractant-directed cell migration, resulted in almost complete loss of directionality, but similar acceleration/deceleration kinetics of migration speed as wild-type cells. These results indicate that Gβ and RasG are required for the induction of migration speed and directionality, respectively, in response to an EF, suggesting separation of migration speed and directionality even with intact feedback loops between mechanical and signaling networks. SN - 2046-6390 UR - https://www.unboundmedicine.com/medline/citation/31221628/Cell_migration_directionality_and_speed_are_independently_regulated_by_RasG_and_Gβ_in_Dictyostelium_cells_in_electrotaxis L2 - http://bio.biologists.org/cgi/pmidlookup?view=long&pmid=31221628 DB - PRIME DP - Unbound Medicine ER -