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High prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae and particularly Trichomonas vaginalis diagnosed using US FDA-approved Aptima molecular tests and evaluation of conventional routine diagnostic tests in Ternopil, Ukraine.
APMIS 2019; 127(9):627-634A

Abstract

Sexually transmitted infections (STIs) remain major public health problems globally. Appropriate laboratory diagnosis of STIs is rare in Ukraine. We investigated the prevalence of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Trichomonas vaginalis (TV) using the US FDA-approved Aptima Combo 2 and Aptima TV assays and compared the results with the conventional routine diagnostic tests (CDTs) in Ukraine. Urogenital swabs from consecutive mostly symptomatic females (n = 296) and males (n = 159) were examined. The prevalences were as follows: 10% (n = 47) of TV, 5.3% (n = 24) of CT and 1.5% (n = 7) of NG. The specificity of some CDTs was high, for example, 100% for NG culture, TV IgG ELISA, CT IgM ELISA and CT microscopy, but lower for other CDTs, that is, from 44% to 99.8%. The sensitivity of all CDTs was suboptimal, that is, 71% (n = 5) for NG microscopy, 57% (n = 4) for NG culture, 53% (n = 8) for CT IgG ELISA, 33% (n = 1) for TV IgG ELISA, 28% (n = 13) for TV microscopy, 25% (n = 1) for CT IgA ELISA, 20% (n = 3) for CT IgM ELISA and 0% (n = 0) for CT microscopy. The prevalences of particularly TV and CT were high, but substantial also for NG, in Ternopil, Ukraine. The sensitivities of all CDTs were low, and widespread implementation of validated, quality-assured and cost-effective molecular diagnostic STI tests in Ukraine is imperative.

Authors+Show Affiliations

Clinical Laboratory Department, Ternopil Regional Clinical Dermatovenerologic Dispensary, Ternopil, Ukraine. Department of Functional and Laboratory Diagnostics, I. Horbachevsky Ternopil State Medical University, Ternopil, Ukraine. WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for STIs, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for STIs, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.Department of Functional and Laboratory Diagnostics, I. Horbachevsky Ternopil State Medical University, Ternopil, Ukraine.WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for STIs, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31225920

Citation

Boiko, Iryna, et al. "High Prevalence of Chlamydia Trachomatis, Neisseria Gonorrhoeae and Particularly Trichomonas Vaginalis Diagnosed Using US FDA-approved Aptima Molecular Tests and Evaluation of Conventional Routine Diagnostic Tests in Ternopil, Ukraine." APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, vol. 127, no. 9, 2019, pp. 627-634.
Boiko I, Golparian D, Krynytska I, et al. High prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae and particularly Trichomonas vaginalis diagnosed using US FDA-approved Aptima molecular tests and evaluation of conventional routine diagnostic tests in Ternopil, Ukraine. APMIS. 2019;127(9):627-634.
Boiko, I., Golparian, D., Krynytska, I., & Unemo, M. (2019). High prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae and particularly Trichomonas vaginalis diagnosed using US FDA-approved Aptima molecular tests and evaluation of conventional routine diagnostic tests in Ternopil, Ukraine. APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, 127(9), pp. 627-634. doi:10.1111/apm.12975.
Boiko I, et al. High Prevalence of Chlamydia Trachomatis, Neisseria Gonorrhoeae and Particularly Trichomonas Vaginalis Diagnosed Using US FDA-approved Aptima Molecular Tests and Evaluation of Conventional Routine Diagnostic Tests in Ternopil, Ukraine. APMIS. 2019;127(9):627-634. PubMed PMID: 31225920.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae and particularly Trichomonas vaginalis diagnosed using US FDA-approved Aptima molecular tests and evaluation of conventional routine diagnostic tests in Ternopil, Ukraine. AU - Boiko,Iryna, AU - Golparian,Daniel, AU - Krynytska,Inna, AU - Unemo,Magnus, PY - 2019/03/22/received PY - 2019/06/17/accepted PY - 2019/6/22/pubmed PY - 2019/8/20/medline PY - 2019/6/22/entrez KW - Chlamydia trachomatis KW - Neisseria gonorrhoeae KW - Trichomonas vaginalis KW - Aptima Combo 2 assay KW - Aptima Trichomonas vaginalis assay KW - Ukraine SP - 627 EP - 634 JF - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica JO - APMIS VL - 127 IS - 9 N2 - Sexually transmitted infections (STIs) remain major public health problems globally. Appropriate laboratory diagnosis of STIs is rare in Ukraine. We investigated the prevalence of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Trichomonas vaginalis (TV) using the US FDA-approved Aptima Combo 2 and Aptima TV assays and compared the results with the conventional routine diagnostic tests (CDTs) in Ukraine. Urogenital swabs from consecutive mostly symptomatic females (n = 296) and males (n = 159) were examined. The prevalences were as follows: 10% (n = 47) of TV, 5.3% (n = 24) of CT and 1.5% (n = 7) of NG. The specificity of some CDTs was high, for example, 100% for NG culture, TV IgG ELISA, CT IgM ELISA and CT microscopy, but lower for other CDTs, that is, from 44% to 99.8%. The sensitivity of all CDTs was suboptimal, that is, 71% (n = 5) for NG microscopy, 57% (n = 4) for NG culture, 53% (n = 8) for CT IgG ELISA, 33% (n = 1) for TV IgG ELISA, 28% (n = 13) for TV microscopy, 25% (n = 1) for CT IgA ELISA, 20% (n = 3) for CT IgM ELISA and 0% (n = 0) for CT microscopy. The prevalences of particularly TV and CT were high, but substantial also for NG, in Ternopil, Ukraine. The sensitivities of all CDTs were low, and widespread implementation of validated, quality-assured and cost-effective molecular diagnostic STI tests in Ukraine is imperative. SN - 1600-0463 UR - https://www.unboundmedicine.com/medline/citation/31225920/High_prevalence_of_Chlamydia_trachomatis_Neisseria_gonorrhoeae_and_particularly_Trichomonas_vaginalis_diagnosed_using_US_FDA_approved_Aptima_molecular_tests_and_evaluation_of_conventional_routine_diagnostic_tests_in_Ternopil_Ukraine_ L2 - https://doi.org/10.1111/apm.12975 DB - PRIME DP - Unbound Medicine ER -