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Antimicrobial activity of red wine and oenological extracts against periodontal pathogens in a validated oral biofilm model.
BMC Complement Altern Med. 2019 Jun 21; 19(1):145.BC

Abstract

BACKGROUND

Previous research findings support an antimicrobial effect of polyphenols against a variety of pathogens, but there is no evidence of this effect against periodontal pathogens in complex biofilms. The purpose of this study was to evaluate the antimicrobial activity of red wine and oenological extracts, rich in polyphenols, against the periodontal pathogens Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum and total bacteria growing in an in vitro oral biofilm static model.

METHODS

A previously validated biofilm model, including Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, F. nucleatum, P. gingivalis and A. actinomycetemcomitans was developed on sterile hydroxyapatite discs. Red wine (and dealcoholized wine), and two polyphenols-rich extracts (from wine and grape seeds) were applied to 72 h biofilms by dipping the discs during 1 and 5 min in the wine solutions and during 30 s and 1 min in the oenological extracts. Resulting biofilms were analyzed by confocal laser scanning microscopy and viable bacteria (colony forming units/mL) were measured by quantitative polymerase chain reaction combined with propidium monoazide. A generalized linear model was constructed to determine the effect of the tested products on the viable bacterial counts of A. actinomycetemcomitans, P. gingivalis and F. nucleatum, as well on the total number of viable bacteria.

RESULTS

The results showed that red wine and dealcoholized red wine caused reduction in viability of total bacteria within the biofilm, with statistically significant reductions in the number of viable P. gingivalis after 1 min (p = 0.008) and in A. actinomycetemcomitans after 5 min of exposure (p = 0.011) with red wine. No evidence of relevant antibacterial effect was observed with the oenological extracts, with statistically significant reductions of F. nucleatum after 30 s of exposure to both oenological extracts (p = 0.001).

CONCLUSIONS

Although moderate, the antimicrobial impact observed in the total bacterial counts and counts of A. actinomycetemcomitans, P. gingivalis and F. nucleatum, encourage further investigations on the potential use of these natural products in the prevention and treatment of periodontal diseases.

Authors+Show Affiliations

ETEP (Etiology and Therapy of Periodontal Diseases) Research Group, University Complutense, Madrid, Spain.ETEP (Etiology and Therapy of Periodontal Diseases) Research Group, University Complutense, Madrid, Spain.Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, Madrid, Spain.Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, Madrid, Spain.ETEP (Etiology and Therapy of Periodontal Diseases) Research Group, University Complutense, Madrid, Spain.Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, Madrid, Spain.ETEP (Etiology and Therapy of Periodontal Diseases) Research Group, University Complutense, Madrid, Spain.ETEP (Etiology and Therapy of Periodontal Diseases) Research Group, University Complutense, Madrid, Spain. davidher@ucm.es. Department of Dental Clinical Specialities (DDCS), Faculty of Odontology, Plaza Ramón y Cajal s/n Ciudad Universitaria, 28040, Madrid, Spain. davidher@ucm.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31226983

Citation

Sánchez, María C., et al. "Antimicrobial Activity of Red Wine and Oenological Extracts Against Periodontal Pathogens in a Validated Oral Biofilm Model." BMC Complementary and Alternative Medicine, vol. 19, no. 1, 2019, p. 145.
Sánchez MC, Ribeiro-Vidal H, Esteban-Fernández A, et al. Antimicrobial activity of red wine and oenological extracts against periodontal pathogens in a validated oral biofilm model. BMC Complement Altern Med. 2019;19(1):145.
Sánchez, M. C., Ribeiro-Vidal, H., Esteban-Fernández, A., Bartolomé, B., Figuero, E., Moreno-Arribas, M. V., Sanz, M., & Herrera, D. (2019). Antimicrobial activity of red wine and oenological extracts against periodontal pathogens in a validated oral biofilm model. BMC Complementary and Alternative Medicine, 19(1), 145. https://doi.org/10.1186/s12906-019-2533-5
Sánchez MC, et al. Antimicrobial Activity of Red Wine and Oenological Extracts Against Periodontal Pathogens in a Validated Oral Biofilm Model. BMC Complement Altern Med. 2019 Jun 21;19(1):145. PubMed PMID: 31226983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial activity of red wine and oenological extracts against periodontal pathogens in a validated oral biofilm model. AU - Sánchez,María C, AU - Ribeiro-Vidal,Honorato, AU - Esteban-Fernández,Adelaida, AU - Bartolomé,Begoña, AU - Figuero,Elena, AU - Moreno-Arribas,María V, AU - Sanz,Mariano, AU - Herrera,David, Y1 - 2019/06/21/ PY - 2018/08/30/received PY - 2019/05/29/accepted PY - 2019/6/23/entrez PY - 2019/6/23/pubmed PY - 2019/9/3/medline KW - A. actinomycetemcomitans KW - F. nucleatum KW - Oenological extracts KW - P. gingivalis KW - Periodontal diseases KW - Polyphenols KW - Red wine SP - 145 EP - 145 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 19 IS - 1 N2 - BACKGROUND: Previous research findings support an antimicrobial effect of polyphenols against a variety of pathogens, but there is no evidence of this effect against periodontal pathogens in complex biofilms. The purpose of this study was to evaluate the antimicrobial activity of red wine and oenological extracts, rich in polyphenols, against the periodontal pathogens Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum and total bacteria growing in an in vitro oral biofilm static model. METHODS: A previously validated biofilm model, including Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, F. nucleatum, P. gingivalis and A. actinomycetemcomitans was developed on sterile hydroxyapatite discs. Red wine (and dealcoholized wine), and two polyphenols-rich extracts (from wine and grape seeds) were applied to 72 h biofilms by dipping the discs during 1 and 5 min in the wine solutions and during 30 s and 1 min in the oenological extracts. Resulting biofilms were analyzed by confocal laser scanning microscopy and viable bacteria (colony forming units/mL) were measured by quantitative polymerase chain reaction combined with propidium monoazide. A generalized linear model was constructed to determine the effect of the tested products on the viable bacterial counts of A. actinomycetemcomitans, P. gingivalis and F. nucleatum, as well on the total number of viable bacteria. RESULTS: The results showed that red wine and dealcoholized red wine caused reduction in viability of total bacteria within the biofilm, with statistically significant reductions in the number of viable P. gingivalis after 1 min (p = 0.008) and in A. actinomycetemcomitans after 5 min of exposure (p = 0.011) with red wine. No evidence of relevant antibacterial effect was observed with the oenological extracts, with statistically significant reductions of F. nucleatum after 30 s of exposure to both oenological extracts (p = 0.001). CONCLUSIONS: Although moderate, the antimicrobial impact observed in the total bacterial counts and counts of A. actinomycetemcomitans, P. gingivalis and F. nucleatum, encourage further investigations on the potential use of these natural products in the prevention and treatment of periodontal diseases. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/31226983/Antimicrobial_activity_of_red_wine_and_oenological_extracts_against_periodontal_pathogens_in_a_validated_oral_biofilm_model_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-019-2533-5 DB - PRIME DP - Unbound Medicine ER -