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Prolactin receptor expression in mouse dorsal root ganglia neuronal subtypes is sex-dependent.
J Neuroendocrinol 2019; 31(8):e12759JN

Abstract

Sensory neurones exhibit sex-dependent responsiveness to prolactin (PRL). This could contribute to sexual dimorphism in pathological pain conditions. The present study aimed to determine the mechanisms underlying sex-dependent PRL sensitivity in sensory neurones. A quantitative reverse transcriptase-polymerase chain reaction shows that prolactin receptor (Prlr) long and short isoform mRNAs are expressed at comparable levels in female and male mouse dorsal root ganglia (DRG). In Prlrcre/+ ;Rosa26LSL-tDTomato/+ reporter mice, percentages of Prlr+ sensory neurones in female and male DRG are also similar. Characterisation of Prlr+ DRG neurones using immunohistochemistry and electrophysiology revealed that Prlr+ DRG neurones are mainly peptidergic nociceptors in females and males. However, sensory neurone type-dependent expression of Prlr is sex dimorphic. Thus, Prlr+ populations fell into three small- and two medium-large-sized sensory neuronal groups. Prlr+ DRG neurones are predominantly medium-large sized in males and are proportionally more comprised of small-sized sensory neurones in females. Specifically, Prlr+ /IB4+ /CGRP+ neurones are four- to five-fold higher in numbers in female DRG. By contrast, Prlr+ /IB4- /CGRP+ /5HT3a+ /NPYR2- are predominant in male DRG. Prlr+ /IB4- /CGRP- , Prlr+ /IB4- /CGRP+ and Prlr+ /IB4- /CGRP+ /NPYR2+ neurones are evenly encountered in female and male DRG. These differences were confirmed using an independently generated single-cell sequencing dataset. Overall, we propose a novel mechanism by which sensory neurone type-dependent expression of Prlr could explain the unique sex dimorphism in responsiveness of nociceptors to PRL.

Authors+Show Affiliations

Department of Endodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.Department of Endodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.School of Behavioral and Brain Sciences and Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, TX, USA.Department of Endodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.Department of Pharmacology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.Inserm U1151, Université Paris Descartes, Paris, France.Centre for Neuroendocrinology and Department of Anatomy, University of Otago School of Biomedical Sciences, Dunedin, New Zealand.Department of Pharmacology and Toxicology, Center for Molecular Signaling (PZMS), Saarland University School of Medicine, Homburg, Germany.School of Behavioral and Brain Sciences and Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, TX, USA.School of Behavioral and Brain Sciences and Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, TX, USA.Department of Endodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA. Department of Pharmacology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31231869

Citation

Patil, Mayur, et al. "Prolactin Receptor Expression in Mouse Dorsal Root Ganglia Neuronal Subtypes Is Sex-dependent." Journal of Neuroendocrinology, vol. 31, no. 8, 2019, pp. e12759.
Patil M, Hovhannisyan AH, Wangzhou A, et al. Prolactin receptor expression in mouse dorsal root ganglia neuronal subtypes is sex-dependent. J Neuroendocrinol. 2019;31(8):e12759.
Patil, M., Hovhannisyan, A. H., Wangzhou, A., Mecklenburg, J., Koek, W., Goffin, V., ... Akopian, A. N. (2019). Prolactin receptor expression in mouse dorsal root ganglia neuronal subtypes is sex-dependent. Journal of Neuroendocrinology, 31(8), pp. e12759. doi:10.1111/jne.12759.
Patil M, et al. Prolactin Receptor Expression in Mouse Dorsal Root Ganglia Neuronal Subtypes Is Sex-dependent. J Neuroendocrinol. 2019;31(8):e12759. PubMed PMID: 31231869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prolactin receptor expression in mouse dorsal root ganglia neuronal subtypes is sex-dependent. AU - Patil,Mayur, AU - Hovhannisyan,Anahit H, AU - Wangzhou,Andi, AU - Mecklenburg,Jennifer, AU - Koek,Wouter, AU - Goffin,Vincent, AU - Grattan,David, AU - Boehm,Ulrich, AU - Dussor,Gregory, AU - Price,Theodore J, AU - Akopian,Armen N, Y1 - 2019/07/04/ PY - 2019/03/18/received PY - 2019/05/10/revised PY - 2019/06/18/accepted PY - 2019/6/25/pubmed PY - 2019/6/25/medline PY - 2019/6/25/entrez KW - electrophysiology KW - nociception KW - prolactin KW - prolactin receptor KW - sensory neurones SP - e12759 EP - e12759 JF - Journal of neuroendocrinology JO - J. Neuroendocrinol. VL - 31 IS - 8 N2 - Sensory neurones exhibit sex-dependent responsiveness to prolactin (PRL). This could contribute to sexual dimorphism in pathological pain conditions. The present study aimed to determine the mechanisms underlying sex-dependent PRL sensitivity in sensory neurones. A quantitative reverse transcriptase-polymerase chain reaction shows that prolactin receptor (Prlr) long and short isoform mRNAs are expressed at comparable levels in female and male mouse dorsal root ganglia (DRG). In Prlrcre/+ ;Rosa26LSL-tDTomato/+ reporter mice, percentages of Prlr+ sensory neurones in female and male DRG are also similar. Characterisation of Prlr+ DRG neurones using immunohistochemistry and electrophysiology revealed that Prlr+ DRG neurones are mainly peptidergic nociceptors in females and males. However, sensory neurone type-dependent expression of Prlr is sex dimorphic. Thus, Prlr+ populations fell into three small- and two medium-large-sized sensory neuronal groups. Prlr+ DRG neurones are predominantly medium-large sized in males and are proportionally more comprised of small-sized sensory neurones in females. Specifically, Prlr+ /IB4+ /CGRP+ neurones are four- to five-fold higher in numbers in female DRG. By contrast, Prlr+ /IB4- /CGRP+ /5HT3a+ /NPYR2- are predominant in male DRG. Prlr+ /IB4- /CGRP- , Prlr+ /IB4- /CGRP+ and Prlr+ /IB4- /CGRP+ /NPYR2+ neurones are evenly encountered in female and male DRG. These differences were confirmed using an independently generated single-cell sequencing dataset. Overall, we propose a novel mechanism by which sensory neurone type-dependent expression of Prlr could explain the unique sex dimorphism in responsiveness of nociceptors to PRL. SN - 1365-2826 UR - https://www.unboundmedicine.com/medline/citation/31231869/Prolactin_receptor_expression_in_mouse_dorsal_root_ganglia_neuronal_subtypes_is_sex-dependent L2 - https://doi.org/10.1111/jne.12759 DB - PRIME DP - Unbound Medicine ER -