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Rolling circle replication of single-stranded DNA plasmid pC194.
EMBO J 1987; 6(12):3863-9EJ

Abstract

A group of small Staphylococcus aureus/Bacillus subtilis plasmids was recently found to replicate via a circular single-stranded DNA intermediate (te Riele et al., 1986a). We show here that a 55 bp region of one such plasmid, pC194, has origin activity when complemented in trans by the plasmid replication protein. This region contains two palindromes, 5 and 14 bp long, and a site nicked by the replication protein. DNA synthesis presumably initiated at the nick in the replication origin can be terminated at an 18 bp sequence homologous to the site of initiation, deriving from another plasmid, pUB110, or synthesized in vitro. This result suggests that, similar to the Escherichia coli single-stranded DNA phages, pC194 replicates as a rolling circle. Interestingly, there is homology between replication origins and replication proteins of pC194 and the phage phi mX174.

Authors+Show Affiliations

Institut Jacques Monod, Paris, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3123220

Citation

Gros, M F., et al. "Rolling Circle Replication of Single-stranded DNA Plasmid PC194." The EMBO Journal, vol. 6, no. 12, 1987, pp. 3863-9.
Gros MF, te Riele H, Ehrlich SD. Rolling circle replication of single-stranded DNA plasmid pC194. EMBO J. 1987;6(12):3863-9.
Gros, M. F., te Riele, H., & Ehrlich, S. D. (1987). Rolling circle replication of single-stranded DNA plasmid pC194. The EMBO Journal, 6(12), pp. 3863-9.
Gros MF, te Riele H, Ehrlich SD. Rolling Circle Replication of Single-stranded DNA Plasmid PC194. EMBO J. 1987 Dec 1;6(12):3863-9. PubMed PMID: 3123220.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rolling circle replication of single-stranded DNA plasmid pC194. AU - Gros,M F, AU - te Riele,H, AU - Ehrlich,S D, PY - 1987/12/1/pubmed PY - 1987/12/1/medline PY - 1987/12/1/entrez SP - 3863 EP - 9 JF - The EMBO journal JO - EMBO J. VL - 6 IS - 12 N2 - A group of small Staphylococcus aureus/Bacillus subtilis plasmids was recently found to replicate via a circular single-stranded DNA intermediate (te Riele et al., 1986a). We show here that a 55 bp region of one such plasmid, pC194, has origin activity when complemented in trans by the plasmid replication protein. This region contains two palindromes, 5 and 14 bp long, and a site nicked by the replication protein. DNA synthesis presumably initiated at the nick in the replication origin can be terminated at an 18 bp sequence homologous to the site of initiation, deriving from another plasmid, pUB110, or synthesized in vitro. This result suggests that, similar to the Escherichia coli single-stranded DNA phages, pC194 replicates as a rolling circle. Interestingly, there is homology between replication origins and replication proteins of pC194 and the phage phi mX174. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/3123220/Rolling_circle_replication_of_single_stranded_DNA_plasmid_pC194_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0261-4189&date=1987&volume=6&issue=12&spage=3863 DB - PRIME DP - Unbound Medicine ER -