Tags

Type your tag names separated by a space and hit enter

A Case of Identity Confirmation of Brucella abortus S99 by Phage Typing and PCR Methods.
Arch Razi Inst 2019; 74(2):127-133AR

Abstract

Brucellosis is a zoonotic infection that is associated with fever in humans and abortion in animals. The agent of this disease is a facultative intracellular gram-negative coccobacillus called Brucella. There are six classic species, including B. abortus, B. melitensis, B. suis, B. canis, B. neotomae, and B. ovis. In recent years, four new species have been reported, including Brucella ceti, B. microti, B. pinnipedialis, and B. inopinata. Human disease causes hygienic and economic losses, including inactivity of workforces in the community and high cost of treatment. The disease also causes catastrophic losses in the livestock industry. There is no effective vaccine against human brucellosis. Hence, attempts to prevent human infection with Brucella are focused on preventative measures, including control of infection in livestock, which lead to a reduction in its incidence in humans. The common methods for diagnosis of this disease are serologic methods including Rose Bengal, Wright -2 ME and the ring test. B. abortus strain S99 is used to produce these diagnostic antigens. The production of these antigens requires the presence of a well-characterized seed with full identity. The aim of this work was confirmation of the identity of B. abortus S99 by phage typing, AMOS and multiplex PCR techniques. Therefore, it is essential to carry out the identification of the strains used as seed for the production of the brucellosis diagnostic antigens. In this project, B. abortus strain 99 was supplied by the bacterial collection of the Brucellosis Department of Razi Vaccine and Serum Research Institute. Then, the main aim of the present study was the confirmation of the seed identity by doing the tests through the standard phage typing method, AMOS PCR and multiplex PCR (Brucladder) methods. Results were in support of the identity of the studied strain, and the molecular methods could also be used as the sensitive approaches for validation of antigenic seed.

Authors+Show Affiliations

Assistant professor at Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. Assistant professor at Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.Assistant professor at Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.Department of Bio bank, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.Brucellosis Department, Razi Vaccine and Serum Research Institute (RVSRI); Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.Brucellosis Department, Razi Vaccine and Serum Research Institute (RVSRI); Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31232562

Citation

Alamian, S, et al. "A Case of Identity Confirmation of Brucella Abortus S99 By Phage Typing and PCR Methods." Archives of Razi Institute, vol. 74, no. 2, 2019, pp. 127-133.
Alamian S, Dadar M, Soleimani S, et al. A Case of Identity Confirmation of Brucella abortus S99 by Phage Typing and PCR Methods. Arch Razi Inst. 2019;74(2):127-133.
Alamian, S., Dadar, M., Soleimani, S., Behrozikhah, A. M., & Etemadi, A. (2019). A Case of Identity Confirmation of Brucella abortus S99 by Phage Typing and PCR Methods. Archives of Razi Institute, 74(2), pp. 127-133. doi:10.22092/ari.2019.123507.1255.
Alamian S, et al. A Case of Identity Confirmation of Brucella Abortus S99 By Phage Typing and PCR Methods. Arch Razi Inst. 2019;74(2):127-133. PubMed PMID: 31232562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Case of Identity Confirmation of Brucella abortus S99 by Phage Typing and PCR Methods. AU - Alamian,S, AU - Dadar,M, AU - Soleimani,S, AU - Behrozikhah,A M, AU - Etemadi,A, Y1 - 2019/06/01/ PY - 2019/01/01/accepted PY - 2018/10/08/received PY - 2019/6/25/entrez KW - Antigenic seed KW - Brucella abortus S99 KW - Molecular test KW - Validation SP - 127 EP - 133 JF - Archives of Razi Institute JO - Arch Razi Inst VL - 74 IS - 2 N2 - Brucellosis is a zoonotic infection that is associated with fever in humans and abortion in animals. The agent of this disease is a facultative intracellular gram-negative coccobacillus called Brucella. There are six classic species, including B. abortus, B. melitensis, B. suis, B. canis, B. neotomae, and B. ovis. In recent years, four new species have been reported, including Brucella ceti, B. microti, B. pinnipedialis, and B. inopinata. Human disease causes hygienic and economic losses, including inactivity of workforces in the community and high cost of treatment. The disease also causes catastrophic losses in the livestock industry. There is no effective vaccine against human brucellosis. Hence, attempts to prevent human infection with Brucella are focused on preventative measures, including control of infection in livestock, which lead to a reduction in its incidence in humans. The common methods for diagnosis of this disease are serologic methods including Rose Bengal, Wright -2 ME and the ring test. B. abortus strain S99 is used to produce these diagnostic antigens. The production of these antigens requires the presence of a well-characterized seed with full identity. The aim of this work was confirmation of the identity of B. abortus S99 by phage typing, AMOS and multiplex PCR techniques. Therefore, it is essential to carry out the identification of the strains used as seed for the production of the brucellosis diagnostic antigens. In this project, B. abortus strain 99 was supplied by the bacterial collection of the Brucellosis Department of Razi Vaccine and Serum Research Institute. Then, the main aim of the present study was the confirmation of the seed identity by doing the tests through the standard phage typing method, AMOS PCR and multiplex PCR (Brucladder) methods. Results were in support of the identity of the studied strain, and the molecular methods could also be used as the sensitive approaches for validation of antigenic seed. SN - 2008-9872 UR - https://www.unboundmedicine.com/medline/citation/31232562/A_Case_of_Identity_Confirmation_of_Brucella_abortus_S99_by_Phage_Typing_and_PCR_Methods L2 - https://doi.org/10.22092/ari.2019.123507.1255 DB - PRIME DP - Unbound Medicine ER -