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Transcriptome analysis identified aberrant gene expression in pollen developmental pathways leading to CGMS in cotton (Gossypium hirsutum L.).

Abstract

Male sterility (induced or natural) is a potential tool for commercial hybrid seed production in different crops. Despite numerous endeavors to understand the physiological, hereditary, and molecular cascade of events governing CMS in cotton, the exact biological process controlling sterility and fertility reconstruction remains obscure. During current study, RNA-Seq using Ion Torrent S5 platform is carried out to identify 'molecular portraits' in floral buds among the Cytoplasmic Genic Male Sterility (CGMS) line, its near-isogenic maintainer, and restorer lines. A total of 300, 438 and 455 genes were differentially expressed in CGMS, Maintainer, and Restorer lines respectively. The functional analysis using AgriGo revealed suppression in the pathways involved in biogenesis and metabolism of secondary metabolites which play an important role in pollen and anther maturation. Enrichment analysis showed dearth related to pollen and anther's development in sterile line, including anomalous expression of genes and transcription factors that have a role in the development of the reproductive organ, abnormal cytoskeleton formation, defects in cell wall formation. The current study found aberrant expression of DYT1, AMS and cytochrome P450 genes involved in tapetum formation, pollen development, pollen exine and anther cuticle formation associated to male sterility as well as fertility restoration of CGMS. In the current study, more numbers of DEGs were found on Chromosome D05 and A05 as compared to other chromosomes. Expression pattern analysis of fourteen randomly selected genes using qRT-PCR showed high concurrence with gene expression profile of RNA-Seq analysis accompanied by a strong correlation of 0.82. The present study provides an important support for future studies in identifying interaction between cyto-nuclear molecular portraits, to accelerate functional genomics and molecular breeding related to cytoplasmic male sterility studies in cotton.

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  • Authors+Show Affiliations

    ,

    Department of Biotechnology and Plant Breeding, Ferdowsi University of Mashhad, Mashhad, Iran.

    ,

    Department of Biotechnology and Plant Breeding, Ferdowsi University of Mashhad, Mashhad, Iran.

    ,

    Department of Biotechnology and Biochemistry, Junagadh Agricultural University, Junagadh, Gujarat, India.

    ,

    Department of Biotechnology and Plant Breeding, Ferdowsi University of Mashhad, Mashhad, Iran.

    Department of Animal Biotechnology, Anand Agricultural University, Anand, Gujarat, India.

    Source

    PloS one 14:6 2019 pg e0218381

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31233531

    Citation

    Hamid, Rasmieh, et al. "Transcriptome Analysis Identified Aberrant Gene Expression in Pollen Developmental Pathways Leading to CGMS in Cotton (Gossypium Hirsutum L.)." PloS One, vol. 14, no. 6, 2019, pp. e0218381.
    Hamid R, Marashi H, Tomar RS, et al. Transcriptome analysis identified aberrant gene expression in pollen developmental pathways leading to CGMS in cotton (Gossypium hirsutum L.). PLoS ONE. 2019;14(6):e0218381.
    Hamid, R., Marashi, H., Tomar, R. S., Malekzadeh Shafaroudi, S., & Sabara, P. H. (2019). Transcriptome analysis identified aberrant gene expression in pollen developmental pathways leading to CGMS in cotton (Gossypium hirsutum L.). PloS One, 14(6), pp. e0218381. doi:10.1371/journal.pone.0218381.
    Hamid R, et al. Transcriptome Analysis Identified Aberrant Gene Expression in Pollen Developmental Pathways Leading to CGMS in Cotton (Gossypium Hirsutum L.). PLoS ONE. 2019;14(6):e0218381. PubMed PMID: 31233531.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Transcriptome analysis identified aberrant gene expression in pollen developmental pathways leading to CGMS in cotton (Gossypium hirsutum L.). AU - Hamid,Rasmieh, AU - Marashi,Hassan, AU - Tomar,Rukam S, AU - Malekzadeh Shafaroudi,Saeid, AU - Sabara,Pritesh H, Y1 - 2019/06/24/ PY - 2019/01/02/received PY - 2019/06/01/accepted PY - 2019/6/25/entrez PY - 2019/6/25/pubmed PY - 2019/6/25/medline SP - e0218381 EP - e0218381 JF - PloS one JO - PLoS ONE VL - 14 IS - 6 N2 - Male sterility (induced or natural) is a potential tool for commercial hybrid seed production in different crops. Despite numerous endeavors to understand the physiological, hereditary, and molecular cascade of events governing CMS in cotton, the exact biological process controlling sterility and fertility reconstruction remains obscure. During current study, RNA-Seq using Ion Torrent S5 platform is carried out to identify 'molecular portraits' in floral buds among the Cytoplasmic Genic Male Sterility (CGMS) line, its near-isogenic maintainer, and restorer lines. A total of 300, 438 and 455 genes were differentially expressed in CGMS, Maintainer, and Restorer lines respectively. The functional analysis using AgriGo revealed suppression in the pathways involved in biogenesis and metabolism of secondary metabolites which play an important role in pollen and anther maturation. Enrichment analysis showed dearth related to pollen and anther's development in sterile line, including anomalous expression of genes and transcription factors that have a role in the development of the reproductive organ, abnormal cytoskeleton formation, defects in cell wall formation. The current study found aberrant expression of DYT1, AMS and cytochrome P450 genes involved in tapetum formation, pollen development, pollen exine and anther cuticle formation associated to male sterility as well as fertility restoration of CGMS. In the current study, more numbers of DEGs were found on Chromosome D05 and A05 as compared to other chromosomes. Expression pattern analysis of fourteen randomly selected genes using qRT-PCR showed high concurrence with gene expression profile of RNA-Seq analysis accompanied by a strong correlation of 0.82. The present study provides an important support for future studies in identifying interaction between cyto-nuclear molecular portraits, to accelerate functional genomics and molecular breeding related to cytoplasmic male sterility studies in cotton. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/31233531/Transcriptome_analysis_identified_aberrant_gene_expression_in_pollen_developmental_pathways_leading_to_CGMS_in_cotton_(Gossypium_hirsutum_L.) L2 - http://dx.plos.org/10.1371/journal.pone.0218381 DB - PRIME DP - Unbound Medicine ER -