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Chromosomal Localization of 18S-28S rDNA and (TTAGGG)n Sequences in Two South African Dormice of the Genus Graphiurus (Rodentia: Gliridae).

Abstract

Classical cytogenetics and mapping of 18S-28S rDNA and (TTAGGG)n sequences by fluorescence in situ hybridization (FISH) was performed on Graphiurus platyops (GPL) and Graphiurus ocularis (GOC) metaphases with the aim to characterize the genomes. In both species, inverted DAPI karyotypes showed the same diploid number, 2n = 46, and hybridization of the (TTAGGG)n probe revealed interstitial telomeric sequences (ITSs) at the centromeres of almost all bi-armed chromosomes. FISH with the rDNA probe localized nucleolus organizer regions (NORs), at the terminal ends of the p arms of the subtelocentric pairs 16 and 17 in both species and detected additional signals on GPL8 and GOC18, 19, and 22. The species have similar karyotypes, but their chromosome pairs 18-22 differ in morphology; these are acrocentric in G. platyops, as also confirmed by C-banding, and subtelocentric in G. ocularis. These differences in pairs 18-22 were also highlighted by hybridization of the telomeric probe (TTAGGG)n, which showed the small p arms in G. ocularis enriched with ITSs. FISH of rDNA probes detected multiple NOR loci in G. ocularis, underlining the intense evolutionary dynamics related to these genes. Although the Graphiurus species analyzed have similar karyotypes, the results on the repetitive sequences indicate a complex pattern of genomic reorganization and evolution occurring in these phylogenetically close species.

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    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31234172

    Citation

    Milioto, Vanessa, et al. "Chromosomal Localization of 18S-28S rDNA and (TTAGGG)n Sequences in Two South African Dormice of the Genus Graphiurus (Rodentia: Gliridae)." Cytogenetic and Genome Research, 2019.
    Milioto V, Vlah S, Mazzoleni S, et al. Chromosomal Localization of 18S-28S rDNA and (TTAGGG)n Sequences in Two South African Dormice of the Genus Graphiurus (Rodentia: Gliridae). Cytogenet Genome Res. 2019.
    Milioto, V., Vlah, S., Mazzoleni, S., Rovatsos, M., & Dumas, F. (2019). Chromosomal Localization of 18S-28S rDNA and (TTAGGG)n Sequences in Two South African Dormice of the Genus Graphiurus (Rodentia: Gliridae). Cytogenetic and Genome Research, doi:10.1159/000500985.
    Milioto V, et al. Chromosomal Localization of 18S-28S rDNA and (TTAGGG)n Sequences in Two South African Dormice of the Genus Graphiurus (Rodentia: Gliridae). Cytogenet Genome Res. 2019 Jun 22; PubMed PMID: 31234172.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Chromosomal Localization of 18S-28S rDNA and (TTAGGG)n Sequences in Two South African Dormice of the Genus Graphiurus (Rodentia: Gliridae). AU - Milioto,Vanessa, AU - Vlah,Sara, AU - Mazzoleni,Sofia, AU - Rovatsos,Michail, AU - Dumas,Francesca, Y1 - 2019/06/22/ PY - 2019/03/06/accepted PY - 2019/6/25/entrez PY - 2019/6/25/pubmed PY - 2019/6/25/medline KW - <italic>Graphiurus ocularis</italic> KW - <italic>Graphiurus platyops</italic> KW - Chromosomal evolution KW - Fusion KW - Karyotypes KW - Repetitive sequence amplification JF - Cytogenetic and genome research JO - Cytogenet. Genome Res. N2 - Classical cytogenetics and mapping of 18S-28S rDNA and (TTAGGG)n sequences by fluorescence in situ hybridization (FISH) was performed on Graphiurus platyops (GPL) and Graphiurus ocularis (GOC) metaphases with the aim to characterize the genomes. In both species, inverted DAPI karyotypes showed the same diploid number, 2n = 46, and hybridization of the (TTAGGG)n probe revealed interstitial telomeric sequences (ITSs) at the centromeres of almost all bi-armed chromosomes. FISH with the rDNA probe localized nucleolus organizer regions (NORs), at the terminal ends of the p arms of the subtelocentric pairs 16 and 17 in both species and detected additional signals on GPL8 and GOC18, 19, and 22. The species have similar karyotypes, but their chromosome pairs 18-22 differ in morphology; these are acrocentric in G. platyops, as also confirmed by C-banding, and subtelocentric in G. ocularis. These differences in pairs 18-22 were also highlighted by hybridization of the telomeric probe (TTAGGG)n, which showed the small p arms in G. ocularis enriched with ITSs. FISH of rDNA probes detected multiple NOR loci in G. ocularis, underlining the intense evolutionary dynamics related to these genes. Although the Graphiurus species analyzed have similar karyotypes, the results on the repetitive sequences indicate a complex pattern of genomic reorganization and evolution occurring in these phylogenetically close species. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/31234172/Chromosomal_Localization_of_18S-28S_rDNA_and_(TTAGGG)n_Sequences_in_Two_South_African_Dormice_of_the_Genus_Graphiurus_(Rodentia:_Gliridae) L2 - https://www.karger.com?DOI=10.1159/000500985 DB - PRIME DP - Unbound Medicine ER -