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Comparison of the Substrate Preferences of ω3 Fatty Acid Desaturases for Long Chain Polyunsaturated Fatty Acids.
Int J Mol Sci. 2019 Jun 22; 20(12)IJ

Abstract

Omega-3 long chain polyunsaturated fatty acids (ω3 LC-PUFAs) such as eicosapentaenoic acid (EPA; 20:5ω3) and docosahexaenoic acid (DHA; 22:6ω3) are important fatty acids for human health. These ω3 LC-PUFAs are produced from their ω3 precursors by a set of desaturases and elongases involved in the biosynthesis pathway and are also converted from ω6 LC-PUFA by omega-3 desaturases (ω3Ds). Here, we have investigated eight ω3-desaturases obtained from a cyanobacterium, plants, fungi and a lower animal species for their activities and compared their specificities for various C18, C20 and C22 ω6 PUFA substrates by transiently expressing them in Nicotiana benthamiana leaves. Our results showed hitherto unreported activity of many of the ω3Ds on ω6 LC-PUFA substrates leading to their conversion to ω3 LC-PUFAs. This discovery could be important in the engineering of EPA and DHA in heterologous hosts.

Authors+Show Affiliations

CSIRO Agriculture & Food, Canberra, ACT 2601, Australia. Pushkar.Shrestha@csiro.au.CSIRO Agriculture & Food, Canberra, ACT 2601, Australia. Xue-Rong.Zhou@csiro.au.CSIRO Agriculture & Food, Canberra, ACT 2601, Australia. Sapna.Vibhakaranpillai@csiro.au.CSIRO Agriculture & Food, Canberra, ACT 2601, Australia. James.Petrie@csiro.au.CSIRO Agriculture & Food, Canberra, ACT 2601, Australia. Robert.Defeyter@csiro.au.CSIRO Agriculture & Food, Canberra, ACT 2601, Australia. Surinder.Singh@csiro.au.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31234541

Citation

Shrestha, Pushkar, et al. "Comparison of the Substrate Preferences of Ω3 Fatty Acid Desaturases for Long Chain Polyunsaturated Fatty Acids." International Journal of Molecular Sciences, vol. 20, no. 12, 2019.
Shrestha P, Zhou XR, Vibhakaran Pillai S, et al. Comparison of the Substrate Preferences of ω3 Fatty Acid Desaturases for Long Chain Polyunsaturated Fatty Acids. Int J Mol Sci. 2019;20(12).
Shrestha, P., Zhou, X. R., Vibhakaran Pillai, S., Petrie, J., de Feyter, R., & Singh, S. (2019). Comparison of the Substrate Preferences of ω3 Fatty Acid Desaturases for Long Chain Polyunsaturated Fatty Acids. International Journal of Molecular Sciences, 20(12). https://doi.org/10.3390/ijms20123058
Shrestha P, et al. Comparison of the Substrate Preferences of Ω3 Fatty Acid Desaturases for Long Chain Polyunsaturated Fatty Acids. Int J Mol Sci. 2019 Jun 22;20(12) PubMed PMID: 31234541.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of the Substrate Preferences of ω3 Fatty Acid Desaturases for Long Chain Polyunsaturated Fatty Acids. AU - Shrestha,Pushkar, AU - Zhou,Xue-Rong, AU - Vibhakaran Pillai,Sapna, AU - Petrie,James, AU - de Feyter,Robert, AU - Singh,Surinder, Y1 - 2019/06/22/ PY - 2019/05/24/received PY - 2019/06/20/revised PY - 2019/06/21/accepted PY - 2019/6/26/entrez PY - 2019/6/27/pubmed PY - 2020/1/21/medline KW - DHA KW - EPA KW - Omega-3 desaturase KW - long-chain polyunsaturated fatty acids KW - substrate specificity JF - International journal of molecular sciences JO - Int J Mol Sci VL - 20 IS - 12 N2 - Omega-3 long chain polyunsaturated fatty acids (ω3 LC-PUFAs) such as eicosapentaenoic acid (EPA; 20:5ω3) and docosahexaenoic acid (DHA; 22:6ω3) are important fatty acids for human health. These ω3 LC-PUFAs are produced from their ω3 precursors by a set of desaturases and elongases involved in the biosynthesis pathway and are also converted from ω6 LC-PUFA by omega-3 desaturases (ω3Ds). Here, we have investigated eight ω3-desaturases obtained from a cyanobacterium, plants, fungi and a lower animal species for their activities and compared their specificities for various C18, C20 and C22 ω6 PUFA substrates by transiently expressing them in Nicotiana benthamiana leaves. Our results showed hitherto unreported activity of many of the ω3Ds on ω6 LC-PUFA substrates leading to their conversion to ω3 LC-PUFAs. This discovery could be important in the engineering of EPA and DHA in heterologous hosts. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/31234541/Comparison_of_the_Substrate_Preferences_of_ω3_Fatty_Acid_Desaturases_for_Long_Chain_Polyunsaturated_Fatty_Acids_ L2 - https://www.mdpi.com/resolver?pii=ijms20123058 DB - PRIME DP - Unbound Medicine ER -