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Identification of Ellagitannins in the Unripe Fruit of Rubus Chingii Hu and Evaluation of its Potential Antidiabetic Activity.
J Agric Food Chem. 2019 Jun 26; 67(25):7025-7039.JA

Abstract

As a functional food, the unripe fruits of Rubus chingii Hu have been widely used in China for thousands of years. Twenty-five major ellagitannins (ETs) were identified from the unripe fruits, and a novel ellagitannin, chingiitannin A (1), together with four other known ETs (2-5) were isolated and identified by HPLC-QTOF-MS/MS and 2D-NMR. Chingiitannin A showed the highest α-glucosidase and α-amylase inhibitory activities (IC50 2.89 and 4.52 μM, respectively), which occurred in a reversible and noncompetitive manner. Static quenching was indicated in a fluorescence quenching assay. Molecular docking results revealed that chingiitannin A interacted with the enzymes mainly by hydrogen bonding and was bound in the allosteric site. Chingiitannin A was nontoxic, and it increased the glucose uptake in L6 myotubes. The results suggested that the unripe fruits of Rubus chingii Hu are rich sources of ETs, and chingiitannin A might be a good candidate for functional foods or antidiabetic drugs.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology , Tianjin University , Tianjin 300072 , P. R. China.

Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology , Tianjin University , Tianjin 300072 , P. R. China.

Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology , Tianjin University , Tianjin 300072 , P. R. China.

Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology , Tianjin University , Tianjin 300072 , P. R. China.

Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology , Tianjin University , Tianjin 300072 , P. R. China.

Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology , Tianjin University , Tianjin 300072 , P. R. China.

Department of Pharmaceutical and Pharmacological Sciences , University of Padua , Via Marzolo , 535131 Padua , Italy.

College of Food Engineering and Biotechnology , Tianjin University of Science and Technology , Tianjin 300457 , P. R. China.

Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology , Tianjin University , Tianjin 300072 , P. R. China.

MeSH

AnimalsCell LineChromatography, High Pressure LiquidFruitGlycoside Hydrolase InhibitorsHydrolyzable TanninsHypoglycemic AgentsKineticsMolecular Docking SimulationMolecular StructureMyoblastsPlant ExtractsRatsRubusTandem Mass Spectrometryalpha-Glucosidases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31240933

Citation

Chen, Yue, et al. "Identification of Ellagitannins in the Unripe Fruit of Rubus Chingii Hu and Evaluation of Its Potential Antidiabetic Activity." Journal of Agricultural and Food Chemistry, vol. 67, no. 25, 2019, pp. 7025-7039.

Chen Y, Chen Z, Guo Q, et al. Identification of Ellagitannins in the Unripe Fruit of Rubus Chingii Hu and Evaluation of its Potential Antidiabetic Activity. J Agric Food Chem. 2019;67(25):7025-7039.

Chen, Y., Chen, Z., Guo, Q., Gao, X., Ma, Q., Xue, Z., Ferri, N., Zhang, M., & Chen, H. (2019). Identification of Ellagitannins in the Unripe Fruit of Rubus Chingii Hu and Evaluation of its Potential Antidiabetic Activity. Journal of Agricultural and Food Chemistry, 67(25), 7025-7039. https://doi.org/10.1021/acs.jafc.9b02293

Chen Y, et al. Identification of Ellagitannins in the Unripe Fruit of Rubus Chingii Hu and Evaluation of Its Potential Antidiabetic Activity. J Agric Food Chem. 2019 Jun 26;67(25):7025-7039. PubMed PMID: 31240933.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Identification of Ellagitannins in the Unripe Fruit of Rubus Chingii Hu and Evaluation of its Potential Antidiabetic Activity. AU - Chen,Yue, AU - Chen,Zhongqin, AU - Guo,Qingwen, AU - Gao,Xudong, AU - Ma,Qiqi, AU - Xue,Zihan, AU - Ferri,Nicola, AU - Zhang,Min, AU - Chen,Haixia, Y1 - 2019/06/18/ PY - 2019/6/27/entrez PY - 2019/6/27/pubmed PY - 2019/7/23/medline KW - HPLC-QTOF-MS/MS KW - NMR KW - Rubus chingii Hu KW - ellagitannins KW - interaction KW - α-glucosidase and α-amylase SP - 7025 EP - 7039 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 67 IS - 25 N2 - As a functional food, the unripe fruits of Rubus chingii Hu have been widely used in China for thousands of years. Twenty-five major ellagitannins (ETs) were identified from the unripe fruits, and a novel ellagitannin, chingiitannin A (1), together with four other known ETs (2-5) were isolated and identified by HPLC-QTOF-MS/MS and 2D-NMR. Chingiitannin A showed the highest α-glucosidase and α-amylase inhibitory activities (IC50 2.89 and 4.52 μM, respectively), which occurred in a reversible and noncompetitive manner. Static quenching was indicated in a fluorescence quenching assay. Molecular docking results revealed that chingiitannin A interacted with the enzymes mainly by hydrogen bonding and was bound in the allosteric site. Chingiitannin A was nontoxic, and it increased the glucose uptake in L6 myotubes. The results suggested that the unripe fruits of Rubus chingii Hu are rich sources of ETs, and chingiitannin A might be a good candidate for functional foods or antidiabetic drugs. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/31240933/Identification_of_Ellagitannins_in_the_Unripe_Fruit_of_Rubus_Chingii_Hu_and_Evaluation_of_its_Potential_Antidiabetic_Activity_ DB - PRIME DP - Unbound Medicine ER -