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In vitro bactericidal activity of levonadifloxacin (WCK 771) against methicillin- and quinolone-resistant Staphylococcus aureus biofilms.
J Med Microbiol. 2019 Aug; 68(8):1129-1136.JM

Abstract

PURPOSE

Staphylococcus aureus causes a wide range of infections, such as endocarditis, pneumonia, osteomyelitis, skin and soft tissue infections, and implant/in-dwelling device-related infections. S. aureus poses a significant challenge to clinicians because of its ability to rapidly acquire multi-drug resistance and quickly progress into a recurrent, chronic infection by biofilm formation. Levonadifloxacin (WCK 771) is a novel broad-spectrum antibacterial agent (it recently completed a phase 3 trial in India) with a differentiated mechanism of action involving high affinity to staphylococcal DNA gyrase, and is active against multi-drug-resistant (MDR) S. aureus, including those that are resistant to quinolones. The present study investigated the bactericidal activity of levonadifloxacin against biofilm-embedded S. aureus clinical isolates in comparison with other anti-S. aureus drugs.

METHODOLOGY

The bactericidal activity of levonadifloxacin and comparator drugs such as vancomycin, linezolid and daptomycin was evaluated against planktonic and biofilm-encapsulated recent methicillin- and quinolone-resistant S. aureus clinical isolates using time-kill, biofilm eradication and scanning electron microscopy analysis.

RESULTS

Levonadifloxacin displayed a consistent ≥90 % bacterial kill rate against biofilm-embedded organisms, while vancomycin and linezolid displayed variable activity and daptomycin did not show any activity. Scanning electron microscopy images further confirmed the efficacy of levonadifloxacin against biofilm, showing the disruption of biofilm structure and a corresponding reduction in the viable bacterial count.

CONCLUSION

These results show that levonadifloxacin has an improved bactericidal effect on biofilm-embedded quinolone-resistant S. aureus and meticillin-resistant S. aureus, and that it can be a promising treatment option for such infections.

Authors+Show Affiliations

Wockhardt Research Centre, Aurangabad, Maharashtra, India.Wockhardt Research Centre, Aurangabad, Maharashtra, India.Wockhardt Research Centre, Aurangabad, Maharashtra, India.Wockhardt Research Centre, Aurangabad, Maharashtra, India.Wockhardt Research Centre, Aurangabad, Maharashtra, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31241446

Citation

Tellis, Melroy, et al. "In Vitro Bactericidal Activity of Levonadifloxacin (WCK 771) Against Methicillin- and Quinolone-resistant Staphylococcus Aureus Biofilms." Journal of Medical Microbiology, vol. 68, no. 8, 2019, pp. 1129-1136.
Tellis M, Joseph J, Khande H, et al. In vitro bactericidal activity of levonadifloxacin (WCK 771) against methicillin- and quinolone-resistant Staphylococcus aureus biofilms. J Med Microbiol. 2019;68(8):1129-1136.
Tellis, M., Joseph, J., Khande, H., Bhagwat, S., & Patel, M. (2019). In vitro bactericidal activity of levonadifloxacin (WCK 771) against methicillin- and quinolone-resistant Staphylococcus aureus biofilms. Journal of Medical Microbiology, 68(8), 1129-1136. https://doi.org/10.1099/jmm.0.000999
Tellis M, et al. In Vitro Bactericidal Activity of Levonadifloxacin (WCK 771) Against Methicillin- and Quinolone-resistant Staphylococcus Aureus Biofilms. J Med Microbiol. 2019;68(8):1129-1136. PubMed PMID: 31241446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro bactericidal activity of levonadifloxacin (WCK 771) against methicillin- and quinolone-resistant Staphylococcus aureus biofilms. AU - Tellis,Melroy, AU - Joseph,Jiji, AU - Khande,Hemant, AU - Bhagwat,Sachin, AU - Patel,Mahesh, Y1 - 2019/06/26/ PY - 2019/6/27/pubmed PY - 2019/8/15/medline PY - 2019/6/27/entrez KW - MDR Gram-positive KW - MRSA KW - QRSA KW - Staphylococcus aureus KW - biofilm KW - levonadifloxacin (WCK 771) SP - 1129 EP - 1136 JF - Journal of medical microbiology JO - J. Med. Microbiol. VL - 68 IS - 8 N2 - PURPOSE: Staphylococcus aureus causes a wide range of infections, such as endocarditis, pneumonia, osteomyelitis, skin and soft tissue infections, and implant/in-dwelling device-related infections. S. aureus poses a significant challenge to clinicians because of its ability to rapidly acquire multi-drug resistance and quickly progress into a recurrent, chronic infection by biofilm formation. Levonadifloxacin (WCK 771) is a novel broad-spectrum antibacterial agent (it recently completed a phase 3 trial in India) with a differentiated mechanism of action involving high affinity to staphylococcal DNA gyrase, and is active against multi-drug-resistant (MDR) S. aureus, including those that are resistant to quinolones. The present study investigated the bactericidal activity of levonadifloxacin against biofilm-embedded S. aureus clinical isolates in comparison with other anti-S. aureus drugs. METHODOLOGY: The bactericidal activity of levonadifloxacin and comparator drugs such as vancomycin, linezolid and daptomycin was evaluated against planktonic and biofilm-encapsulated recent methicillin- and quinolone-resistant S. aureus clinical isolates using time-kill, biofilm eradication and scanning electron microscopy analysis. RESULTS: Levonadifloxacin displayed a consistent ≥90 % bacterial kill rate against biofilm-embedded organisms, while vancomycin and linezolid displayed variable activity and daptomycin did not show any activity. Scanning electron microscopy images further confirmed the efficacy of levonadifloxacin against biofilm, showing the disruption of biofilm structure and a corresponding reduction in the viable bacterial count. CONCLUSION: These results show that levonadifloxacin has an improved bactericidal effect on biofilm-embedded quinolone-resistant S. aureus and meticillin-resistant S. aureus, and that it can be a promising treatment option for such infections. SN - 1473-5644 UR - https://www.unboundmedicine.com/medline/citation/31241446/In_vitro_bactericidal_activity_of_levonadifloxacin__WCK_771__against_methicillin__and_quinolone_resistant_Staphylococcus_aureus_biofilms_ L2 - http://jmm.microbiologyresearch.org/pubmed/content/journal/jmm/10.1099/jmm.0.000999 DB - PRIME DP - Unbound Medicine ER -