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mRNA expression profile of bronchoalveolar lavage fluid cells from patients with idiopathic pulmonary fibrosis and sarcoidosis.
Eur J Clin Invest. 2019 Sep; 49(9):e13153.EJ

Abstract

BACKGROUND

Sarcoidosis and idiopathic pulmonary fibrosis (IPF) are two most frequent forms of interstitial lung diseases (ILDs). Cellular and biochemical composition of bronchoalveolar lavage fluid (BALf) was shown to reflect the fibrotic changes in the lung. However, the usefulness of BALf cellular profile evaluation in the diagnosis of ILDs is limited. The aim of the study was a multivariate, molecular analysis of BALf cells from IPF and sarcoidosis patients.

METHODS

Transcriptomic measurements were carried out using Affymetrix Human Gene 2.1 ST ArrayStrip in 21 samples: 9 IPF and 12 sarcoidosis. The mRNA expression for the most significantly differentiating genes was evaluated by real-time PCR in 32 samples (11 IPF and 21 sarcoidosis).

RESULTS

The number of genes differentially expressed between IPF and sarcoidosis groups was 4832 (13359 probesets). Cluster analysis indicated that sarcoidosis BALf cells are characterized by increased mRNA expression of genes associated with ribosome biogenesis. Clusters formed by genes with changed mRNA expression in IPF samples were implicated in the processes of cell adhesion and migration, metalloproteinase expression and negative regulation of cell proliferation. The GO analysis indicated that predominant biological processes associated with the differential mRNA gene expression of BALf cells were upregulation of neutrophils in IPF and lymphocytes in sarcoidosis.

CONCLUSIONS

Analysis of BALf from sarcoidosis and IPF showed highly different mRNA profile of cells. The most important biological processes observed at the molecular level in BALf cells were associated with ribosome biogenesis and proteasome apparatus in sarcoidosis and neutrophilic dysfunction in IPF.

Authors+Show Affiliations

Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, Warsaw, Poland.Department of Genetics, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland.Postgraduate School of Molecular Medicine, Medical University of Warsaw, Warsaw, Poland.Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, Warsaw, Poland.Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, Warsaw, Poland.Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, Warsaw, Poland.Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, Warsaw, Poland.Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, Warsaw, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31246273

Citation

Paplińska-Goryca, Magdalena, et al. "MRNA Expression Profile of Bronchoalveolar Lavage Fluid Cells From Patients With Idiopathic Pulmonary Fibrosis and Sarcoidosis." European Journal of Clinical Investigation, vol. 49, no. 9, 2019, pp. e13153.
Paplińska-Goryca M, Goryca K, Misiukiewicz-Stępień P, et al. MRNA expression profile of bronchoalveolar lavage fluid cells from patients with idiopathic pulmonary fibrosis and sarcoidosis. Eur J Clin Invest. 2019;49(9):e13153.
Paplińska-Goryca, M., Goryca, K., Misiukiewicz-Stępień, P., Nejman-Gryz, P., Proboszcz, M., Górska, K., Maskey-Warzęchowska, M., & Krenke, R. (2019). MRNA expression profile of bronchoalveolar lavage fluid cells from patients with idiopathic pulmonary fibrosis and sarcoidosis. European Journal of Clinical Investigation, 49(9), e13153. https://doi.org/10.1111/eci.13153
Paplińska-Goryca M, et al. MRNA Expression Profile of Bronchoalveolar Lavage Fluid Cells From Patients With Idiopathic Pulmonary Fibrosis and Sarcoidosis. Eur J Clin Invest. 2019;49(9):e13153. PubMed PMID: 31246273.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - mRNA expression profile of bronchoalveolar lavage fluid cells from patients with idiopathic pulmonary fibrosis and sarcoidosis. AU - Paplińska-Goryca,Magdalena, AU - Goryca,Krzysztof, AU - Misiukiewicz-Stępień,Paulina, AU - Nejman-Gryz,Patrycja, AU - Proboszcz,Małgorzata, AU - Górska,Katarzyna, AU - Maskey-Warzęchowska,Marta, AU - Krenke,Rafał, Y1 - 2019/07/11/ PY - 2019/01/23/received PY - 2019/06/25/accepted PY - 2019/6/28/pubmed PY - 2020/6/2/medline PY - 2019/6/28/entrez KW - bronchoalveolar lavage fluid KW - idiopathic pulmonary fibrosis KW - interstitial lung diseases KW - mRNA KW - sarcoidosis SP - e13153 EP - e13153 JF - European journal of clinical investigation JO - Eur. J. Clin. Invest. VL - 49 IS - 9 N2 - BACKGROUND: Sarcoidosis and idiopathic pulmonary fibrosis (IPF) are two most frequent forms of interstitial lung diseases (ILDs). Cellular and biochemical composition of bronchoalveolar lavage fluid (BALf) was shown to reflect the fibrotic changes in the lung. However, the usefulness of BALf cellular profile evaluation in the diagnosis of ILDs is limited. The aim of the study was a multivariate, molecular analysis of BALf cells from IPF and sarcoidosis patients. METHODS: Transcriptomic measurements were carried out using Affymetrix Human Gene 2.1 ST ArrayStrip in 21 samples: 9 IPF and 12 sarcoidosis. The mRNA expression for the most significantly differentiating genes was evaluated by real-time PCR in 32 samples (11 IPF and 21 sarcoidosis). RESULTS: The number of genes differentially expressed between IPF and sarcoidosis groups was 4832 (13359 probesets). Cluster analysis indicated that sarcoidosis BALf cells are characterized by increased mRNA expression of genes associated with ribosome biogenesis. Clusters formed by genes with changed mRNA expression in IPF samples were implicated in the processes of cell adhesion and migration, metalloproteinase expression and negative regulation of cell proliferation. The GO analysis indicated that predominant biological processes associated with the differential mRNA gene expression of BALf cells were upregulation of neutrophils in IPF and lymphocytes in sarcoidosis. CONCLUSIONS: Analysis of BALf from sarcoidosis and IPF showed highly different mRNA profile of cells. The most important biological processes observed at the molecular level in BALf cells were associated with ribosome biogenesis and proteasome apparatus in sarcoidosis and neutrophilic dysfunction in IPF. SN - 1365-2362 UR - https://www.unboundmedicine.com/medline/citation/31246273/mRNA_expression_profile_of_bronchoalveolar_lavage_fluid_cells_from_patients_with_idiopathic_pulmonary_fibrosis_and_sarcoidosis_ DB - PRIME DP - Unbound Medicine ER -