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In vitro Post-Antifungal Effect of Posaconazole and Its Impact on Adhesion-Related Traits and Hemolysin Production of Oral Candida dubliniensis Isolates.
Med Princ Pract. 2019; 28(6):552-558.MP

Abstract

OBJECTIVE

Candidal adherence to denture acrylic surfaces (DAS) and oral buccal epithelial cells (BEC), formation of candidal germ tubes (GT), candidal cell surface hydrophobicity (CSH), and hemolysin production are important pathogenic traits of Candida. The antifungal drug-induced post-antifungal effect (PAFE) also impacts the virulence of Candida. Candida dubliniensis isolates are associated with the causation of oral candidiasis which could be managed with posaconazole. Thus far there is no evidence on posaconazole-induced PAFE and its impact on adhesion-related attributes and production of hemolysin by C. dubliniensis isolates. Hence, the PAFE, adhesion to DAS and BEC, formation of GT, CSH, and hemolysin production of 20 oral C. dubliniensis isolates after brief exposure to posaconazole was ascertained.

MATERIALS AND METHODS

The PAFE, adherence to DAS and BEC, formation of GT, candidal CSH, and hemolysin production were investigated by hitherto described in vitro assays.

RESULTS

The mean PAFE (h) induced by posaconazole on C. dubliniensis isolates was 1.66. Exposure to posaconazole suppressed the ability of C. dubliniensis to adhere to DAS, BEC, formation of candidal GT, candidal CSH and to produce hemolysin by a reduction of 44, 33, 34, 36, and 15% (p < 0.005 to p < 0.001), respectively.

CONCLUSION

Exposure of C. dubliniensis isolates to posaconazole for a brief period induced an antimycotic impact by subduing its growth in addition to suppressing pathogenic adherence-associated attributes, as well as production of hemolysin.

Authors+Show Affiliations

Faculty of Dentistry, Health Sciences Center, Kuwait University, Kuwait, Kuwait, arjuna@hsc.edu.kw.Faculty of Science, University of Colombo, Colombo, Sri Lanka.Faculty of Medicine, Health Sciences Center, Kuwait University, Kuwait, Kuwait.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31247626

Citation

Ellepola, Arjuna Nishantha Bandara, et al. "In Vitro Post-Antifungal Effect of Posaconazole and Its Impact On Adhesion-Related Traits and Hemolysin Production of Oral Candida Dubliniensis Isolates." Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre, vol. 28, no. 6, 2019, pp. 552-558.
Ellepola ANB, Dassanayake RS, Khan Z. In vitro Post-Antifungal Effect of Posaconazole and Its Impact on Adhesion-Related Traits and Hemolysin Production of Oral Candida dubliniensis Isolates. Med Princ Pract. 2019;28(6):552-558.
Ellepola, A. N. B., Dassanayake, R. S., & Khan, Z. (2019). In vitro Post-Antifungal Effect of Posaconazole and Its Impact on Adhesion-Related Traits and Hemolysin Production of Oral Candida dubliniensis Isolates. Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre, 28(6), 552-558. https://doi.org/10.1159/000501764
Ellepola ANB, Dassanayake RS, Khan Z. In Vitro Post-Antifungal Effect of Posaconazole and Its Impact On Adhesion-Related Traits and Hemolysin Production of Oral Candida Dubliniensis Isolates. Med Princ Pract. 2019;28(6):552-558. PubMed PMID: 31247626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro Post-Antifungal Effect of Posaconazole and Its Impact on Adhesion-Related Traits and Hemolysin Production of Oral Candida dubliniensis Isolates. AU - Ellepola,Arjuna Nishantha Bandara, AU - Dassanayake,Ranil Samantha, AU - Khan,Ziauddin, Y1 - 2019/06/28/ PY - 2019/01/28/received PY - 2019/06/27/accepted PY - 2019/6/28/pubmed PY - 2020/6/13/medline PY - 2019/6/28/entrez KW - Adhesion KW - Candida dubliniensis KW - Hemolysin KW - Posaconazole KW - Post-antifungal effect SP - 552 EP - 558 JF - Medical principles and practice : international journal of the Kuwait University, Health Science Centre JO - Med Princ Pract VL - 28 IS - 6 N2 - OBJECTIVE: Candidal adherence to denture acrylic surfaces (DAS) and oral buccal epithelial cells (BEC), formation of candidal germ tubes (GT), candidal cell surface hydrophobicity (CSH), and hemolysin production are important pathogenic traits of Candida. The antifungal drug-induced post-antifungal effect (PAFE) also impacts the virulence of Candida. Candida dubliniensis isolates are associated with the causation of oral candidiasis which could be managed with posaconazole. Thus far there is no evidence on posaconazole-induced PAFE and its impact on adhesion-related attributes and production of hemolysin by C. dubliniensis isolates. Hence, the PAFE, adhesion to DAS and BEC, formation of GT, CSH, and hemolysin production of 20 oral C. dubliniensis isolates after brief exposure to posaconazole was ascertained. MATERIALS AND METHODS: The PAFE, adherence to DAS and BEC, formation of GT, candidal CSH, and hemolysin production were investigated by hitherto described in vitro assays. RESULTS: The mean PAFE (h) induced by posaconazole on C. dubliniensis isolates was 1.66. Exposure to posaconazole suppressed the ability of C. dubliniensis to adhere to DAS, BEC, formation of candidal GT, candidal CSH and to produce hemolysin by a reduction of 44, 33, 34, 36, and 15% (p < 0.005 to p < 0.001), respectively. CONCLUSION: Exposure of C. dubliniensis isolates to posaconazole for a brief period induced an antimycotic impact by subduing its growth in addition to suppressing pathogenic adherence-associated attributes, as well as production of hemolysin. SN - 1423-0151 UR - https://www.unboundmedicine.com/medline/citation/31247626/In-vitro_Post-antifungal_Effect_of_Posaconazole_and_its_Impact_on_Adhesion_related_Traits_and_Hemolysin_Production_of_Oral_Candida_dubliniensis_Isolates L2 - https://www.karger.com?DOI=10.1159/000501764 DB - PRIME DP - Unbound Medicine ER -