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Comparison of multiple doses of cyclosporine A on germ cell apoptosis and epididymal sperm parameters after testicular ischemia/reperfusion in rats.
Exp Mol Pathol. 2019 10; 110:104271.EM

Abstract

Testicular torsion/detorsion (T/D) is an inflammatory problem in men genital system with infertility effects. Cyclosporine A (CsA) as an immunosuppressant medication, exerts anti-inflammatory properties in tissue injuries. We sought to compare the efficacy of 3 doses of CsA on oxidative stress, apoptosis and epididymal sperm quality after ipsilateral testicular T/D.

METHODS

96 mature male rats were divided into six groups 16 each in: Control group (Group1), Sham operated (Group2), In rest groups, the right testis was twisted 720° in a clockwise direction for 1 h; T/D + 0.1% dimethylsulfoxide) DMSO((Group3), and in groups 4-6; CsA were administered 1, 5, and 10 mg/kg, intravenously (iv) 30 and 90 min after torsion, respectively.

RESULTS

Tissue malondialdehyde (MDA) level and caspase-3 activity increased and catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities decreased in compared with control group 4 h after detorsion (p < .001). In six rats of each group 24 h after detorsion, histopathological changes and germ cell apoptosis were significantly deteriorated by determining mean of seminiferous tubules diameters (MSTD) and TUNEL assay. Moreover, 30 days after T/D, sperm concentration and motility were examined in rest of animals.

CONCLUSIONS

Pre- and post-reperfusion CsA diminished MDA and caspase-3levels and normalized antioxidant enzymes activities. Germ cell apoptosis was significantly reduced, as well as, MSTD and long-term sperm insults were improved. Inhibition of mitochondrial permeability transition pore opening is suggested mechanism for cell protection against testicular T/D insults.

Authors+Show Affiliations

Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Cellular and Molecular Biology, Faculty of Basic Science, University of Maragheh, Maragheh, Iran.Department of Pharmacology and Toxicology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran.Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: dehpour@yahoo.com.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31251898

Citation

Yazdani, Iraj, et al. "Comparison of Multiple Doses of Cyclosporine a On Germ Cell Apoptosis and Epididymal Sperm Parameters After Testicular Ischemia/reperfusion in Rats." Experimental and Molecular Pathology, vol. 110, 2019, p. 104271.
Yazdani I, Majdani R, Ghasemnejad-Berenji M, et al. Comparison of multiple doses of cyclosporine A on germ cell apoptosis and epididymal sperm parameters after testicular ischemia/reperfusion in rats. Exp Mol Pathol. 2019;110:104271.
Yazdani, I., Majdani, R., Ghasemnejad-Berenji, M., & Dehpour, A. R. (2019). Comparison of multiple doses of cyclosporine A on germ cell apoptosis and epididymal sperm parameters after testicular ischemia/reperfusion in rats. Experimental and Molecular Pathology, 110, 104271. https://doi.org/10.1016/j.yexmp.2019.104271
Yazdani I, et al. Comparison of Multiple Doses of Cyclosporine a On Germ Cell Apoptosis and Epididymal Sperm Parameters After Testicular Ischemia/reperfusion in Rats. Exp Mol Pathol. 2019;110:104271. PubMed PMID: 31251898.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of multiple doses of cyclosporine A on germ cell apoptosis and epididymal sperm parameters after testicular ischemia/reperfusion in rats. AU - Yazdani,Iraj, AU - Majdani,Raheleh, AU - Ghasemnejad-Berenji,Morteza, AU - Dehpour,Ahmad Reza, Y1 - 2019/06/25/ PY - 2018/11/11/received PY - 2019/05/20/revised PY - 2019/06/14/accepted PY - 2019/6/30/pubmed PY - 2020/2/26/medline PY - 2019/6/29/entrez KW - Apoptosis KW - Cyclosporine A KW - Germ cell KW - Mitochondrial permeability transition pore KW - Testicular T/D SP - 104271 EP - 104271 JF - Experimental and molecular pathology JO - Exp Mol Pathol VL - 110 N2 - : Testicular torsion/detorsion (T/D) is an inflammatory problem in men genital system with infertility effects. Cyclosporine A (CsA) as an immunosuppressant medication, exerts anti-inflammatory properties in tissue injuries. We sought to compare the efficacy of 3 doses of CsA on oxidative stress, apoptosis and epididymal sperm quality after ipsilateral testicular T/D. METHODS: 96 mature male rats were divided into six groups 16 each in: Control group (Group1), Sham operated (Group2), In rest groups, the right testis was twisted 720° in a clockwise direction for 1 h; T/D + 0.1% dimethylsulfoxide) DMSO((Group3), and in groups 4-6; CsA were administered 1, 5, and 10 mg/kg, intravenously (iv) 30 and 90 min after torsion, respectively. RESULTS: Tissue malondialdehyde (MDA) level and caspase-3 activity increased and catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities decreased in compared with control group 4 h after detorsion (p < .001). In six rats of each group 24 h after detorsion, histopathological changes and germ cell apoptosis were significantly deteriorated by determining mean of seminiferous tubules diameters (MSTD) and TUNEL assay. Moreover, 30 days after T/D, sperm concentration and motility were examined in rest of animals. CONCLUSIONS: Pre- and post-reperfusion CsA diminished MDA and caspase-3levels and normalized antioxidant enzymes activities. Germ cell apoptosis was significantly reduced, as well as, MSTD and long-term sperm insults were improved. Inhibition of mitochondrial permeability transition pore opening is suggested mechanism for cell protection against testicular T/D insults. SN - 1096-0945 UR - https://www.unboundmedicine.com/medline/citation/31251898/Comparison_of_multiple_doses_of_cyclosporine_A_on_germ_cell_apoptosis_and_epididymal_sperm_parameters_after_testicular_ischemia/reperfusion_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4800(18)30536-7 DB - PRIME DP - Unbound Medicine ER -