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Lychnophora pinaster ethanolic extract and its chemical constituents ameliorate hyperuricemia and related inflammation.
J Ethnopharmacol. 2019 Oct 05; 242:112040.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Lychnophora pinaster, known as "Brazilian arnica" is used in folk medicine as alcoholic extract to treat inflammation, pain, rheumatism and bruises.

AIM OF THE STUDY

Evaluate the effects of the Lychnophora pinaster's ethanolic extract and its chemical constituents on inflammation and hyperuricemia.

MATERIALS AND METHODS

Ethanolic and hexanic extracts were obtained from the aerial parts of L. pinaster. Sesquiterpene E-lychnophoric acid was isolated from hexanic extract and identified by RMN, GC/MS and IR. In vivo anti-hyperuricemic and anti-inflammatory effects of the ethanolic extracts from L. pinaster (40, 125, 375 mg/kg), E-lychnophoric acid and other constituents previous isolated from L. pinaster and identified in the ethanolic extract by HPLC/UV/DAD (rutin, quercetin and vitexina flavonoids, caffeic, cinnamic and chlorogenic acids, lupeol and stigmasterol, at dose of 15 mg/kg) were assayed by experimental model of oxonate-induced hyperuricemia in Swiss mice, liver xanthine oxidase (XOD) inhibition and by MSU-induced paw edema in mice.

RESULTS

Ethanolic extract and all its components presented anti-hyperuricemic activity by inhibiting the hepatic xanthine oxidase activity. Ethanolic extract and its chemical constituents, except quercetin and vitexin, were able to reduce paw edema size induced by urate crystals. Hypouricemic and anti-inflammatory results obtained for the ethanolic extract (40, 125, 375 mg/kg) and E-lychnophoric acid (15 mg/kg) were similar those obtained for standard drugs, allopurinol (10 mg/kg) and indomethacin (3 mg/kg).

CONCLUSION

Ethanolic extract and E-lychnophoric, chlorogenic, cinnamic and caffeic acids, rutin, lupeol and stigmasterol presented anti-inflammatory and anti-hyperuricemic actvities. These compounds are responsible for the activities presented by the ethanolic extract of L. pinaster. Ethanolic extract and its chemical constituents can be considered promising agents in the therapeutic of inflammation, hyperuricemia and gout.

Authors+Show Affiliations

Laboratory of Medicinal Plants (LAPLAMED), School of Pharmacy, Federal University of Ouro Preto, Minas Gerais state, Brazil.Laboratory of Medicinal Plants (LAPLAMED), School of Pharmacy, Federal University of Ouro Preto, Minas Gerais state, Brazil.Laboratory of Medicinal Plants (LAPLAMED), School of Pharmacy, Federal University of Ouro Preto, Minas Gerais state, Brazil.Laboratory of Medicinal Plants (LAPLAMED), School of Pharmacy, Federal University of Ouro Preto, Minas Gerais state, Brazil. Electronic address: saude@ufop.edu.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31252094

Citation

Martins de Sá Müller, Camila, et al. "Lychnophora Pinaster Ethanolic Extract and Its Chemical Constituents Ameliorate Hyperuricemia and Related Inflammation." Journal of Ethnopharmacology, vol. 242, 2019, p. 112040.
Martins de Sá Müller C, Coelho GB, Carolina de Paula Michel Araújo M, et al. Lychnophora pinaster ethanolic extract and its chemical constituents ameliorate hyperuricemia and related inflammation. J Ethnopharmacol. 2019;242:112040.
Martins de Sá Müller, C., Coelho, G. B., Carolina de Paula Michel Araújo, M., & Saúde-Guimarães, D. A. (2019). Lychnophora pinaster ethanolic extract and its chemical constituents ameliorate hyperuricemia and related inflammation. Journal of Ethnopharmacology, 242, 112040. https://doi.org/10.1016/j.jep.2019.112040
Martins de Sá Müller C, et al. Lychnophora Pinaster Ethanolic Extract and Its Chemical Constituents Ameliorate Hyperuricemia and Related Inflammation. J Ethnopharmacol. 2019 Oct 5;242:112040. PubMed PMID: 31252094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lychnophora pinaster ethanolic extract and its chemical constituents ameliorate hyperuricemia and related inflammation. AU - Martins de Sá Müller,Camila, AU - Coelho,Grazielle Brandão, AU - Carolina de Paula Michel Araújo,Marcela, AU - Saúde-Guimarães,Dênia Antunes, Y1 - 2019/06/25/ PY - 2018/12/18/received PY - 2019/06/18/revised PY - 2019/06/23/accepted PY - 2019/6/30/pubmed PY - 2020/1/29/medline PY - 2019/6/29/entrez KW - Brazilian arnica KW - E-lychnophoric acid KW - Gout KW - Hyperuricemia KW - Inflammation KW - Xanthine oxidase SP - 112040 EP - 112040 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 242 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Lychnophora pinaster, known as "Brazilian arnica" is used in folk medicine as alcoholic extract to treat inflammation, pain, rheumatism and bruises. AIM OF THE STUDY: Evaluate the effects of the Lychnophora pinaster's ethanolic extract and its chemical constituents on inflammation and hyperuricemia. MATERIALS AND METHODS: Ethanolic and hexanic extracts were obtained from the aerial parts of L. pinaster. Sesquiterpene E-lychnophoric acid was isolated from hexanic extract and identified by RMN, GC/MS and IR. In vivo anti-hyperuricemic and anti-inflammatory effects of the ethanolic extracts from L. pinaster (40, 125, 375 mg/kg), E-lychnophoric acid and other constituents previous isolated from L. pinaster and identified in the ethanolic extract by HPLC/UV/DAD (rutin, quercetin and vitexina flavonoids, caffeic, cinnamic and chlorogenic acids, lupeol and stigmasterol, at dose of 15 mg/kg) were assayed by experimental model of oxonate-induced hyperuricemia in Swiss mice, liver xanthine oxidase (XOD) inhibition and by MSU-induced paw edema in mice. RESULTS: Ethanolic extract and all its components presented anti-hyperuricemic activity by inhibiting the hepatic xanthine oxidase activity. Ethanolic extract and its chemical constituents, except quercetin and vitexin, were able to reduce paw edema size induced by urate crystals. Hypouricemic and anti-inflammatory results obtained for the ethanolic extract (40, 125, 375 mg/kg) and E-lychnophoric acid (15 mg/kg) were similar those obtained for standard drugs, allopurinol (10 mg/kg) and indomethacin (3 mg/kg). CONCLUSION: Ethanolic extract and E-lychnophoric, chlorogenic, cinnamic and caffeic acids, rutin, lupeol and stigmasterol presented anti-inflammatory and anti-hyperuricemic actvities. These compounds are responsible for the activities presented by the ethanolic extract of L. pinaster. Ethanolic extract and its chemical constituents can be considered promising agents in the therapeutic of inflammation, hyperuricemia and gout. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/31252094/Lychnophora_pinaster_ethanolic_extract_and_its_chemical_constituents_ameliorate_hyperuricemia_and_related_inflammation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(18)34859-1 DB - PRIME DP - Unbound Medicine ER -