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Novel Ehrlichia sp. detected in Magellanic penguins (Sphenicus magellanicus) and in the seabird tick Ixodes uriae from Magdalena Island, southern Chile.
Ticks Tick Borne Dis. 2019 10; 10(6):101256.TT

Abstract

Ehrlichia spp. are obligatory intracellular microorganisms that infect hematopoietic, endothelial or blood cells of mammals. Ticks are the only vectors of these agents in nature. To date, the role of birds and their associated ticks as reservoirs of ehrlichiae remains almost unexplored. In this study, we performed a molecular screening for bacteria of Anaplasmataceae family in samples of spleen (n = 72) and lung (n = 17), recovered from 72 carcasses of Magellanic penguins (Spheniscus magellanicus) in Brazil and Chile. One apparently unengorged tick (Ixodes uriae) was also collected while wandering upon one of the carcasses and submitted to molecular analyses as well. Through conventional and nested PCR protocols three genes (16S rRNA, dsb and groEL) of a new Ehrlichia sp. were partially characterized upon organs of three penguins and in the tick coming from Magdalena Island (Chile). First matches after BLASTn comparisons showed that our sequences share 99.4% (16S rRNA), 94.6% (groEL) and 79.3% (dsb) of identity with "Candidatus Ehrlichia ornithorhynchi", Ehrlichia sp. NS101 and Ehrlichia canis CCZ, respectively. Matrixes of genetic distance including other representatives of the Ehrlichia genus point a 99.4%, 94.0%, and 80.0% of identity with 16S rRNA, groEL and dsb genes from Ehrlichia sp. It25, Ehrlichia sp. NS101, and Ehrlichia chaffeensis San Louis, respectively. A Bayesian phylogenetic analysis of Anaplasmataceae 16S rRNA gene places the detected Ehrlichia sp. into a group with Ehrlichia sp. BAT and Ehrlichia sp. Natal. Although depicting different topologies, Bayesian unrooted phylogenetic trees constructed for groEL and dsb genes position this Ehrlichia sp. into well-supported branches, which reinforces the finding of a new taxon. For the moment, any pathogenic effect of this new Ehrlichia sp. on penguins is still unknown. However, this fact becomes important to assess from a conservation point of view since populations of Magellanic penguins are currently threatened and in an ongoing decrease.

Authors+Show Affiliations

Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP, 05508-270, Brazil. Electronic address: munoz-leal@usp.br.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP, 05508-270, Brazil.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP, 05508-270, Brazil.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP, 05508-270, Brazil; RRDM - FURG - Programa de Monitoramento da Biodiversidade Aquática na Área Ambiental I - Av. Itália km 8 S/N, Campus Carreiros, 96203-900, Rio Grande, RS, Brazil.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP, 05508-270, Brazil.Instituto de Pesquisa e Reabilitação de Animais Marinhos (IPRAM), Rodovia BR 262 Km 0 sem numero, CEP 29140130, Cariacica, ES, Brazil.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP, 05508-270, Brazil.Laboratorio de Parásitos y Enfermedades de Fauna Silvestre, Departamento de Ciencia Animal, Facultad de Ciencias Veterinarias, Universidad de Concepción, Av. Vicente Méndez 595, Chillán, Chile.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP, 05508-270, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31255535

Citation

Muñoz-Leal, Sebastián, et al. "Novel Ehrlichia Sp. Detected in Magellanic Penguins (Sphenicus Magellanicus) and in the Seabird Tick Ixodes Uriae From Magdalena Island, Southern Chile." Ticks and Tick-borne Diseases, vol. 10, no. 6, 2019, p. 101256.
Muñoz-Leal S, Clemes YS, Lopes MG, et al. Novel Ehrlichia sp. detected in Magellanic penguins (Sphenicus magellanicus) and in the seabird tick Ixodes uriae from Magdalena Island, southern Chile. Ticks Tick Borne Dis. 2019;10(6):101256.
Muñoz-Leal, S., Clemes, Y. S., Lopes, M. G., Acosta, I. C. L., Serpa, M. C. A., Mayorga, L. F. S. P., Gennari, S. M., González-Acuña, D., & Labruna, M. B. (2019). Novel Ehrlichia sp. detected in Magellanic penguins (Sphenicus magellanicus) and in the seabird tick Ixodes uriae from Magdalena Island, southern Chile. Ticks and Tick-borne Diseases, 10(6), 101256. https://doi.org/10.1016/j.ttbdis.2019.06.015
Muñoz-Leal S, et al. Novel Ehrlichia Sp. Detected in Magellanic Penguins (Sphenicus Magellanicus) and in the Seabird Tick Ixodes Uriae From Magdalena Island, Southern Chile. Ticks Tick Borne Dis. 2019;10(6):101256. PubMed PMID: 31255535.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel Ehrlichia sp. detected in Magellanic penguins (Sphenicus magellanicus) and in the seabird tick Ixodes uriae from Magdalena Island, southern Chile. AU - Muñoz-Leal,Sebastián, AU - Clemes,Yara S, AU - Lopes,Marcos G, AU - Acosta,Igor C L, AU - Serpa,Maria Carolina A, AU - Mayorga,Luis Felipe S P, AU - Gennari,Solange M, AU - González-Acuña,Daniel, AU - Labruna,Marcelo B, Y1 - 2019/06/25/ PY - 2019/03/20/received PY - 2019/06/12/revised PY - 2019/06/24/accepted PY - 2019/7/1/pubmed PY - 2020/1/31/medline PY - 2019/7/1/entrez KW - Anaplasmataceae KW - Chile KW - Ehrlichia KW - Ixodes KW - Seabird SP - 101256 EP - 101256 JF - Ticks and tick-borne diseases JO - Ticks Tick Borne Dis VL - 10 IS - 6 N2 - Ehrlichia spp. are obligatory intracellular microorganisms that infect hematopoietic, endothelial or blood cells of mammals. Ticks are the only vectors of these agents in nature. To date, the role of birds and their associated ticks as reservoirs of ehrlichiae remains almost unexplored. In this study, we performed a molecular screening for bacteria of Anaplasmataceae family in samples of spleen (n = 72) and lung (n = 17), recovered from 72 carcasses of Magellanic penguins (Spheniscus magellanicus) in Brazil and Chile. One apparently unengorged tick (Ixodes uriae) was also collected while wandering upon one of the carcasses and submitted to molecular analyses as well. Through conventional and nested PCR protocols three genes (16S rRNA, dsb and groEL) of a new Ehrlichia sp. were partially characterized upon organs of three penguins and in the tick coming from Magdalena Island (Chile). First matches after BLASTn comparisons showed that our sequences share 99.4% (16S rRNA), 94.6% (groEL) and 79.3% (dsb) of identity with "Candidatus Ehrlichia ornithorhynchi", Ehrlichia sp. NS101 and Ehrlichia canis CCZ, respectively. Matrixes of genetic distance including other representatives of the Ehrlichia genus point a 99.4%, 94.0%, and 80.0% of identity with 16S rRNA, groEL and dsb genes from Ehrlichia sp. It25, Ehrlichia sp. NS101, and Ehrlichia chaffeensis San Louis, respectively. A Bayesian phylogenetic analysis of Anaplasmataceae 16S rRNA gene places the detected Ehrlichia sp. into a group with Ehrlichia sp. BAT and Ehrlichia sp. Natal. Although depicting different topologies, Bayesian unrooted phylogenetic trees constructed for groEL and dsb genes position this Ehrlichia sp. into well-supported branches, which reinforces the finding of a new taxon. For the moment, any pathogenic effect of this new Ehrlichia sp. on penguins is still unknown. However, this fact becomes important to assess from a conservation point of view since populations of Magellanic penguins are currently threatened and in an ongoing decrease. SN - 1877-9603 UR - https://www.unboundmedicine.com/medline/citation/31255535/Novel_Ehrlichia_sp__detected_in_Magellanic_penguins__Sphenicus_magellanicus__and_in_the_seabird_tick_Ixodes_uriae_from_Magdalena_Island_southern_Chile_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1877-959X(19)30125-6 DB - PRIME DP - Unbound Medicine ER -