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Novel 3D Printed Device for Dual-Signaling Ratiometric Photoelectrochemical Readout of Biomarker Using λ-Exonuclease-Assisted Recycling Amplification.
Anal Chem. 2019 08 06; 91(15):10049-10055.AC

Abstract

A ratiometric photoelectrochemical (PEC) sensing strategy was proposed for monitoring of carcinoembryonic antigen (CEA) based on a homemade 3D printing device with dual-working photoelectrodes (PE1 and PE2), coupling λ-exonuclease (λ-Exo)-assisted recycling amplification with CdS quantum dots. Gold nanoparticles-functionalized ZnO nanorods were utilized as PEC substrate for generating initial photocurrent and immobilizing DNA probe. Upon incubation of target with DNA trigger/CEA aptamer-modified magnetic bead (tri/apt-MB), DNA trigger dissociated from magnetic bead and then hybridized with capture probe (cp) on PE1 or opened hairpin probe (hp) on PE2 to form double-stranded DNA (dsDNA). The exonuclease could recognize and cleave two newly generated dsDNA, leading to the release of trigger. The free trigger strand continued to hybridize with the remaining cp/hp, which were cleaved by λ-Exo, and then trigger was released again and restarted next recycle with the λ-Exo. After digestion of λ-Exo, the number of capture probes on PE1 was reduced, and many short DNA fragments were produced on PE2, thereby resulting in the decreasing CdS QDs on PE1 and the increasing CdS QDs on PE2. As a result, it was observed that the ratio value of photocurrents (PE1/PE2) significantly decreased with the increasing CEA. Under optimum conditions, the sensing method showed a good linear relationship toward CEA within the dynamic range of 0.02-10 ng mL-1 and a detection limit of 6.0 pg mL-1. Moreover, the ratiometric PEC sensor exhibited good reproducibility, satisfying stability, and remarkable anti-interference performance, which suggests its promising application prospect to detect target CEA.

Authors+Show Affiliations

Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry , Fuzhou University , Fuzhou 350108 , People's Republic of China.Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry , Fuzhou University , Fuzhou 350108 , People's Republic of China.Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry , Fuzhou University , Fuzhou 350108 , People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31256583

Citation

Zhang, Kangyao, et al. "Novel 3D Printed Device for Dual-Signaling Ratiometric Photoelectrochemical Readout of Biomarker Using λ-Exonuclease-Assisted Recycling Amplification." Analytical Chemistry, vol. 91, no. 15, 2019, pp. 10049-10055.
Zhang K, Lv S, Tang D. Novel 3D Printed Device for Dual-Signaling Ratiometric Photoelectrochemical Readout of Biomarker Using λ-Exonuclease-Assisted Recycling Amplification. Anal Chem. 2019;91(15):10049-10055.
Zhang, K., Lv, S., & Tang, D. (2019). Novel 3D Printed Device for Dual-Signaling Ratiometric Photoelectrochemical Readout of Biomarker Using λ-Exonuclease-Assisted Recycling Amplification. Analytical Chemistry, 91(15), 10049-10055. https://doi.org/10.1021/acs.analchem.9b01958
Zhang K, Lv S, Tang D. Novel 3D Printed Device for Dual-Signaling Ratiometric Photoelectrochemical Readout of Biomarker Using λ-Exonuclease-Assisted Recycling Amplification. Anal Chem. 2019 08 6;91(15):10049-10055. PubMed PMID: 31256583.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel 3D Printed Device for Dual-Signaling Ratiometric Photoelectrochemical Readout of Biomarker Using λ-Exonuclease-Assisted Recycling Amplification. AU - Zhang,Kangyao, AU - Lv,Shuzhen, AU - Tang,Dianping, Y1 - 2019/07/11/ PY - 2019/7/2/pubmed PY - 2020/9/17/medline PY - 2019/7/2/entrez SP - 10049 EP - 10055 JF - Analytical chemistry JO - Anal Chem VL - 91 IS - 15 N2 - A ratiometric photoelectrochemical (PEC) sensing strategy was proposed for monitoring of carcinoembryonic antigen (CEA) based on a homemade 3D printing device with dual-working photoelectrodes (PE1 and PE2), coupling λ-exonuclease (λ-Exo)-assisted recycling amplification with CdS quantum dots. Gold nanoparticles-functionalized ZnO nanorods were utilized as PEC substrate for generating initial photocurrent and immobilizing DNA probe. Upon incubation of target with DNA trigger/CEA aptamer-modified magnetic bead (tri/apt-MB), DNA trigger dissociated from magnetic bead and then hybridized with capture probe (cp) on PE1 or opened hairpin probe (hp) on PE2 to form double-stranded DNA (dsDNA). The exonuclease could recognize and cleave two newly generated dsDNA, leading to the release of trigger. The free trigger strand continued to hybridize with the remaining cp/hp, which were cleaved by λ-Exo, and then trigger was released again and restarted next recycle with the λ-Exo. After digestion of λ-Exo, the number of capture probes on PE1 was reduced, and many short DNA fragments were produced on PE2, thereby resulting in the decreasing CdS QDs on PE1 and the increasing CdS QDs on PE2. As a result, it was observed that the ratio value of photocurrents (PE1/PE2) significantly decreased with the increasing CEA. Under optimum conditions, the sensing method showed a good linear relationship toward CEA within the dynamic range of 0.02-10 ng mL-1 and a detection limit of 6.0 pg mL-1. Moreover, the ratiometric PEC sensor exhibited good reproducibility, satisfying stability, and remarkable anti-interference performance, which suggests its promising application prospect to detect target CEA. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/31256583/Novel_3D_Printed_Device_for_Dual_Signaling_Ratiometric_Photoelectrochemical_Readout_of_Biomarker_Using_λ_Exonuclease_Assisted_Recycling_Amplification_ DB - PRIME DP - Unbound Medicine ER -