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The complete mitochondrial genome of Paragonimus ohirai (Paragonimidae: Trematoda: Platyhelminthes) and its comparison with P. westermani congeners and other trematodes.
PeerJ 2019; 7:e7031P

Abstract

We present the complete mitochondrial genome of Paragonimus ohirai Miyazaki, 1939 and compare its features with those of previously reported mitochondrial genomes of the pathogenic lung-fluke, Paragonimus westermani, and other members of the genus. The circular mitochondrial DNA molecule of the single fully sequenced individual of P. ohirai was 14,818 bp in length, containing 12 protein-coding, two ribosomal RNA and 22 transfer RNA genes. As is common among trematodes, an atp8 gene was absent from the mitogenome of P. ohirai and the 5' end of nad4 overlapped with the 3' end of nad4L by 40 bp. Paragonimusohirai and four forms/strains of P. westermani from South Korea and India, exhibited remarkably different base compositions and hence codon usage in protein-coding genes. In the fully sequenced P. ohirai individual, the non-coding region started with two long identical repeats (292 bp each), separated by tRNAGlu . These were followed by an array of six short tandem repeats (STR), 117 bp each. Numbers of the short tandem repeats varied among P. ohirai individuals. A phylogenetic tree inferred from concatenated mitochondrial protein sequences of 50 strains encompassing 42 species of trematodes belonging to 14 families identified a monophyletic Paragonimidae in the class Trematoda. Characterization of additional mitogenomes in the genus Paragonimus will be useful for biomedical studies and development of molecular tools and mitochondrial markers for diagnostic, identification, hybridization and phylogenetic/epidemiological/evolutionary studies.

Authors+Show Affiliations

Immunology Department, Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam. Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.Immunology Department, Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.Immunology Department, Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.Immunology Department, Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam. Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.Department of Environmental Medicine, Kochi Medical School, Kochi University, Oko, Nankoku City, Kochi, Japan.College of Science and Engineering, James Cook University, Townsville, Australia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31259095

Citation

Le, Thanh Hoa, et al. "The Complete Mitochondrial Genome of Paragonimus Ohirai (Paragonimidae: Trematoda: Platyhelminthes) and Its Comparison With P. Westermani Congeners and Other Trematodes." PeerJ, vol. 7, 2019, pp. e7031.
Le TH, Nguyen KT, Nguyen NTB, et al. The complete mitochondrial genome of Paragonimus ohirai (Paragonimidae: Trematoda: Platyhelminthes) and its comparison with P. westermani congeners and other trematodes. PeerJ. 2019;7:e7031.
Le, T. H., Nguyen, K. T., Nguyen, N. T. B., Doan, H. T. T., Agatsuma, T., & Blair, D. (2019). The complete mitochondrial genome of Paragonimus ohirai (Paragonimidae: Trematoda: Platyhelminthes) and its comparison with P. westermani congeners and other trematodes. PeerJ, 7, pp. e7031. doi:10.7717/peerj.7031.
Le TH, et al. The Complete Mitochondrial Genome of Paragonimus Ohirai (Paragonimidae: Trematoda: Platyhelminthes) and Its Comparison With P. Westermani Congeners and Other Trematodes. PeerJ. 2019;7:e7031. PubMed PMID: 31259095.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The complete mitochondrial genome of Paragonimus ohirai (Paragonimidae: Trematoda: Platyhelminthes) and its comparison with P. westermani congeners and other trematodes. AU - Le,Thanh Hoa, AU - Nguyen,Khue Thi, AU - Nguyen,Nga Thi Bich, AU - Doan,Huong Thi Thanh, AU - Agatsuma,Takeshi, AU - Blair,David, Y1 - 2019/06/20/ PY - 2019/02/07/received PY - 2019/04/27/accepted PY - 2019/7/2/entrez PY - 2019/7/2/pubmed PY - 2019/7/2/medline KW - Mitochondrial genome KW - Paragonimidae KW - Paragonimus ohirai KW - Paragonimus westermani KW - Phylogenetic analysis KW - Repeats KW - Skewness value SP - e7031 EP - e7031 JF - PeerJ JO - PeerJ VL - 7 N2 - We present the complete mitochondrial genome of Paragonimus ohirai Miyazaki, 1939 and compare its features with those of previously reported mitochondrial genomes of the pathogenic lung-fluke, Paragonimus westermani, and other members of the genus. The circular mitochondrial DNA molecule of the single fully sequenced individual of P. ohirai was 14,818 bp in length, containing 12 protein-coding, two ribosomal RNA and 22 transfer RNA genes. As is common among trematodes, an atp8 gene was absent from the mitogenome of P. ohirai and the 5' end of nad4 overlapped with the 3' end of nad4L by 40 bp. Paragonimusohirai and four forms/strains of P. westermani from South Korea and India, exhibited remarkably different base compositions and hence codon usage in protein-coding genes. In the fully sequenced P. ohirai individual, the non-coding region started with two long identical repeats (292 bp each), separated by tRNAGlu . These were followed by an array of six short tandem repeats (STR), 117 bp each. Numbers of the short tandem repeats varied among P. ohirai individuals. A phylogenetic tree inferred from concatenated mitochondrial protein sequences of 50 strains encompassing 42 species of trematodes belonging to 14 families identified a monophyletic Paragonimidae in the class Trematoda. Characterization of additional mitogenomes in the genus Paragonimus will be useful for biomedical studies and development of molecular tools and mitochondrial markers for diagnostic, identification, hybridization and phylogenetic/epidemiological/evolutionary studies. SN - 2167-8359 UR - https://www.unboundmedicine.com/medline/citation/31259095/The_complete_mitochondrial_genome_of_Paragonimus_ohirai_(Paragonimidae:_Trematoda:_Platyhelminthes)_and_its_comparison_with_P._westermani_congeners_and_other_trematodes L2 - https://doi.org/10.7717/peerj.7031 DB - PRIME DP - Unbound Medicine ER -