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Mucosal Monosaccharide Transporter Expression in Newborns With Jejunoileal Atresia and Along the Adult Intestine.
J Pediatr Gastroenterol Nutr. 2019 11; 69(5):611-618.JP

Abstract

OBJECTIVES

In newborn rodents, intestinal maturation involves delayed fructose transporter GLUT5 expression until weaning. In jejunoileal atresia (JIA), distal intestinal segments lack exposure to amniotic fluid-containing carbohydrates. We assessed in human newborns, the impact of intestinal maturation and obstruction on mucosal monosaccharide transporter expression.

METHODS

Samples were obtained from 10 newborns operated for small intestinal atresia and from 17 adults undergoing gastroduodenoscopy and/or ileocolonoscopy. mRNA expression of the transporters SGLT1, GLUT1, GLUT2, GLUT5, and GLUT7 was measured in neonate samples proximal and distal of the atresia as well as in adult duodenum, ileum, and colon. Protein expression and localization was assessed using immunofluorescence.

RESULTS

Although mRNA expression of monosaccharide transporters did not significantly differ between newborn and adult samples, luminal fructose transporter GLUT5 protein was absent in 0- to 4-day-old neonates, but expressed in adults. The mRNA expression of the 5 tested monosaccharide transporters was unchanged distal from the JIA relative to proximal. Similarly, luminal sodium-dependent glucose transporter SGLT1 and basolateral GLUT2 were expressed proximal and distal to JIA as visualized by immunofluorescence staining. With the exception of glucose transporter GLUT1 that showed highest expression levels in colon, all investigated hexose transporters showed strongest expression in duodenum, lower levels in ileum and lowest in colon.

CONCLUSIONS

Human newborns lack small intestinal fructose transporter GLUT5 protein expression and small intestinal atresia does not affect the expression of hexose transporters.

Authors+Show Affiliations

Institute of Physiology and Zurich Center for Integrative Human Physiology, University of Zurich (UZH), Zurich.Institute of Physiology and Zurich Center for Integrative Human Physiology, University of Zurich (UZH), Zurich.Institute of Physiology and Zurich Center for Integrative Human Physiology, University of Zurich (UZH), Zurich.Institute of Physiology and Zurich Center for Integrative Human Physiology, University of Zurich (UZH), Zurich.Department of Pediatric Surgery, University Children's Hospital Basel (UKBB), Basel, Switzerland.Institute of Physiology and Zurich Center for Integrative Human Physiology, University of Zurich (UZH), Zurich.Institute of Physiology and Zurich Center for Integrative Human Physiology, University of Zurich (UZH), Zurich. Department of Pediatric Surgery, University Children's Hospital Basel (UKBB), Basel, Switzerland.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31261244

Citation

Meier, Chantal Florence, et al. "Mucosal Monosaccharide Transporter Expression in Newborns With Jejunoileal Atresia and Along the Adult Intestine." Journal of Pediatric Gastroenterology and Nutrition, vol. 69, no. 5, 2019, pp. 611-618.
Meier CF, Camargo SMR, Hunziker S, et al. Mucosal Monosaccharide Transporter Expression in Newborns With Jejunoileal Atresia and Along the Adult Intestine. J Pediatr Gastroenterol Nutr. 2019;69(5):611-618.
Meier, C. F., Camargo, S. M. R., Hunziker, S., Leu, S., Holland-Cunz, S. G., Verrey, F., & Vuille-Dit-Bille, R. N. (2019). Mucosal Monosaccharide Transporter Expression in Newborns With Jejunoileal Atresia and Along the Adult Intestine. Journal of Pediatric Gastroenterology and Nutrition, 69(5), 611-618. https://doi.org/10.1097/MPG.0000000000002425
Meier CF, et al. Mucosal Monosaccharide Transporter Expression in Newborns With Jejunoileal Atresia and Along the Adult Intestine. J Pediatr Gastroenterol Nutr. 2019;69(5):611-618. PubMed PMID: 31261244.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mucosal Monosaccharide Transporter Expression in Newborns With Jejunoileal Atresia and Along the Adult Intestine. AU - Meier,Chantal Florence, AU - Camargo,Simone Mafalda Rodrigues, AU - Hunziker,Schirin, AU - Leu,Svenja, AU - Holland-Cunz,Stefan Gerhard, AU - Verrey,François, AU - Vuille-Dit-Bille,Raphael Nicolas, PY - 2019/7/2/pubmed PY - 2019/7/2/medline PY - 2019/7/2/entrez SP - 611 EP - 618 JF - Journal of pediatric gastroenterology and nutrition JO - J. Pediatr. Gastroenterol. Nutr. VL - 69 IS - 5 N2 - OBJECTIVES: In newborn rodents, intestinal maturation involves delayed fructose transporter GLUT5 expression until weaning. In jejunoileal atresia (JIA), distal intestinal segments lack exposure to amniotic fluid-containing carbohydrates. We assessed in human newborns, the impact of intestinal maturation and obstruction on mucosal monosaccharide transporter expression. METHODS: Samples were obtained from 10 newborns operated for small intestinal atresia and from 17 adults undergoing gastroduodenoscopy and/or ileocolonoscopy. mRNA expression of the transporters SGLT1, GLUT1, GLUT2, GLUT5, and GLUT7 was measured in neonate samples proximal and distal of the atresia as well as in adult duodenum, ileum, and colon. Protein expression and localization was assessed using immunofluorescence. RESULTS: Although mRNA expression of monosaccharide transporters did not significantly differ between newborn and adult samples, luminal fructose transporter GLUT5 protein was absent in 0- to 4-day-old neonates, but expressed in adults. The mRNA expression of the 5 tested monosaccharide transporters was unchanged distal from the JIA relative to proximal. Similarly, luminal sodium-dependent glucose transporter SGLT1 and basolateral GLUT2 were expressed proximal and distal to JIA as visualized by immunofluorescence staining. With the exception of glucose transporter GLUT1 that showed highest expression levels in colon, all investigated hexose transporters showed strongest expression in duodenum, lower levels in ileum and lowest in colon. CONCLUSIONS: Human newborns lack small intestinal fructose transporter GLUT5 protein expression and small intestinal atresia does not affect the expression of hexose transporters. SN - 1536-4801 UR - https://www.unboundmedicine.com/medline/citation/31261244/Mucosal_Monosaccharide_Transporter_Expression_in_Newborns_with_Jejunoileal_Atresia_and_Along_the_Adult_Intestine L2 - https://doi.org/10.1097/MPG.0000000000002425 DB - PRIME DP - Unbound Medicine ER -
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