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(-)-menthol increases excitatory transmission by activating both TRPM8 and TRPA1 channels in mouse spinal lamina II layer.
Biochem Biophys Res Commun. 2019 08 27; 516(3):825-830.BB

Abstract

(-)-menthol, a major form of menthol, is one of the most commonly used chemicals. Many studies have demonstrated that (-)-menthol produces analgesic action through peripheral mechanisms which are mainly mediated by activation of TRPM8. Moreover, intrathecal injection of menthol induces analgesia as well. However, the central actions and mechanisms of (-)-menthol remain unclear. Here, we have investigated the action of (-)-menthol on excitatory synaptic transmission in spinal lamina II layer which plays a pivotal role in modulating nociceptive transmission from the periphery by using patch-clamp technique in mice spinal cord. We found that (-)-menthol increased miniature excitatory postsynaptic current frequency. The frequency increases which (-)-menthol induced were in a dose-dependent manner (EC50: 0.1079 mM). However, neither genetic knockout nor pharmacological inhibition of TRPM8 could block (-)-menthol-induced effects entirely. Furthermore, this increase was also impaired by TRPA1 antagonist HC030031, but abolished utterly by co-application of TRPM8 and TRPA1 antagonist. Our results indicate that (-)-menthol increases the excitatory synaptic transmission by activating either TRPA1 or TRPM8 channels in spinal lamina II layer.

Authors+Show Affiliations

Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen, 518060, China.Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen, 518060, China.Center for Experimental Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China.Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen, 518060, China.Center for Experimental Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China.Department of Endocrinology & Metabolism, Shenzhen University General Hospital and Shenzhen University Academy of Clinical Medical Sciences, Shenzhen University, Shenzhen, 518060, China.Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen, 518060, China.Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen, 518060, China.School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen, 518060, China.Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen, 518060, China.Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen, 518060, China. Electronic address: changyujiang@email.szu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31262448

Citation

Luo, Yuhui, et al. "(-)-menthol Increases Excitatory Transmission By Activating Both TRPM8 and TRPA1 Channels in Mouse Spinal Lamina II Layer." Biochemical and Biophysical Research Communications, vol. 516, no. 3, 2019, pp. 825-830.
Luo Y, Sun W, Feng X, et al. (-)-menthol increases excitatory transmission by activating both TRPM8 and TRPA1 channels in mouse spinal lamina II layer. Biochem Biophys Res Commun. 2019;516(3):825-830.
Luo, Y., Sun, W., Feng, X., Ba, X., Liu, T., Guo, J., Xiao, L., Jiang, J., Hao, Y., Xiong, D., & Jiang, C. (2019). (-)-menthol increases excitatory transmission by activating both TRPM8 and TRPA1 channels in mouse spinal lamina II layer. Biochemical and Biophysical Research Communications, 516(3), 825-830. https://doi.org/10.1016/j.bbrc.2019.06.135
Luo Y, et al. (-)-menthol Increases Excitatory Transmission By Activating Both TRPM8 and TRPA1 Channels in Mouse Spinal Lamina II Layer. Biochem Biophys Res Commun. 2019 08 27;516(3):825-830. PubMed PMID: 31262448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - (-)-menthol increases excitatory transmission by activating both TRPM8 and TRPA1 channels in mouse spinal lamina II layer. AU - Luo,Yuhui, AU - Sun,Wuping, AU - Feng,Xiaojin, AU - Ba,Xiyuan, AU - Liu,Tao, AU - Guo,Jing, AU - Xiao,Lizu, AU - Jiang,Jin, AU - Hao,Yue, AU - Xiong,Donglin, AU - Jiang,Changyu, Y1 - 2019/06/28/ PY - 2019/06/08/received PY - 2019/06/23/accepted PY - 2019/7/3/pubmed PY - 2020/7/10/medline PY - 2019/7/3/entrez KW - (−)-menthol KW - Spinal cord KW - Synaptic transmission KW - TRP channels SP - 825 EP - 830 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 516 IS - 3 N2 - (-)-menthol, a major form of menthol, is one of the most commonly used chemicals. Many studies have demonstrated that (-)-menthol produces analgesic action through peripheral mechanisms which are mainly mediated by activation of TRPM8. Moreover, intrathecal injection of menthol induces analgesia as well. However, the central actions and mechanisms of (-)-menthol remain unclear. Here, we have investigated the action of (-)-menthol on excitatory synaptic transmission in spinal lamina II layer which plays a pivotal role in modulating nociceptive transmission from the periphery by using patch-clamp technique in mice spinal cord. We found that (-)-menthol increased miniature excitatory postsynaptic current frequency. The frequency increases which (-)-menthol induced were in a dose-dependent manner (EC50: 0.1079 mM). However, neither genetic knockout nor pharmacological inhibition of TRPM8 could block (-)-menthol-induced effects entirely. Furthermore, this increase was also impaired by TRPA1 antagonist HC030031, but abolished utterly by co-application of TRPM8 and TRPA1 antagonist. Our results indicate that (-)-menthol increases the excitatory synaptic transmission by activating either TRPA1 or TRPM8 channels in spinal lamina II layer. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/31262448/____menthol_increases_excitatory_transmission_by_activating_both_TRPM8_and_TRPA1_channels_in_mouse_spinal_lamina_II_layer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(19)31273-2 DB - PRIME DP - Unbound Medicine ER -