Tags

Type your tag names separated by a space and hit enter

The Small RNA PinT Contributes to PhoP-Mediated Regulation of the Salmonella Pathogenicity Island 1 Type III Secretion System in Salmonella enterica Serovar Typhimurium.
J Bacteriol. 2019 10 01; 201(19)JB

Abstract

Salmonella enterica serovar Typhimurium induces inflammatory diarrhea and bacterial uptake into intestinal epithelial cells using the Salmonella pathogenicity island 1 (SPI1) type III secretion system (T3SS). HilA activates transcription of the SPI1 structural components and effector proteins. Expression of hilA is activated by HilD, HilC, and RtsA, which act in a complex feed-forward regulatory loop. Many environmental signals and other regulators are integrated into this regulatory loop, primarily via HilD. After the invasion of Salmonella into host intestinal epithelial cells or during systemic replication in macrophages, the SPI T3SS is no longer required or expressed. We have shown that the two-component regulatory system PhoPQ, required for intracellular survival, represses the SPI1 T3SS mostly by controlling the transcription of hilA and hilD Here we show that PinT, one of the PhoPQ-regulated small RNAs (sRNAs), contributes to this regulation by repressing hilA and rtsA translation. PinT base pairs with both the hilA and rtsA mRNAs, resulting in translational inhibition of hilA, but also induces degradation of the rts transcript. PinT also indirectly represses expression of FliZ, a posttranslational regulator of HilD, and directly represses translation of ssrB, encoding the primary regulator of the SPI2 T3SS. Our in vivo mouse competition assays support the concept that PinT controls a series of virulence genes at the posttranscriptional level in order to adapt Salmonella from the invasion stage to intracellular survival.IMPORTANCESalmonella is one of the most important food-borne pathogens, infecting over one million people in the United States every year. These bacteria use a needle-like device to interact with intestinal epithelial cells, leading to invasion of the cells and induction of inflammatory diarrhea. A complex regulatory network controls expression of the invasion system in response to numerous environmental signals. Here we explore the molecular mechanisms by which the small RNA PinT contributes to this regulation, facilitating inactivation of the system after invasion. PinT controls several important virulence systems in Salmonella, tuning the transition between different stages of infection.

Authors+Show Affiliations

Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA slauch@illinois.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

31262841

Citation

Kim, Kyungsub, et al. "The Small RNA PinT Contributes to PhoP-Mediated Regulation of the Salmonella Pathogenicity Island 1 Type III Secretion System in Salmonella Enterica Serovar Typhimurium." Journal of Bacteriology, vol. 201, no. 19, 2019.
Kim K, Palmer AD, Vanderpool CK, et al. The Small RNA PinT Contributes to PhoP-Mediated Regulation of the Salmonella Pathogenicity Island 1 Type III Secretion System in Salmonella enterica Serovar Typhimurium. J Bacteriol. 2019;201(19).
Kim, K., Palmer, A. D., Vanderpool, C. K., & Slauch, J. M. (2019). The Small RNA PinT Contributes to PhoP-Mediated Regulation of the Salmonella Pathogenicity Island 1 Type III Secretion System in Salmonella enterica Serovar Typhimurium. Journal of Bacteriology, 201(19). https://doi.org/10.1128/JB.00312-19
Kim K, et al. The Small RNA PinT Contributes to PhoP-Mediated Regulation of the Salmonella Pathogenicity Island 1 Type III Secretion System in Salmonella Enterica Serovar Typhimurium. J Bacteriol. 2019 10 1;201(19) PubMed PMID: 31262841.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Small RNA PinT Contributes to PhoP-Mediated Regulation of the Salmonella Pathogenicity Island 1 Type III Secretion System in Salmonella enterica Serovar Typhimurium. AU - Kim,Kyungsub, AU - Palmer,Alexander D, AU - Vanderpool,Carin K, AU - Slauch,James M, Y1 - 2019/09/06/ PY - 2019/04/30/received PY - 2019/06/26/accepted PY - 2020/03/06/pmc-release PY - 2019/7/3/pubmed PY - 2020/6/5/medline PY - 2019/7/3/entrez KW - PhoPQ KW - SPI1 KW - Salmonella KW - sRNA JF - Journal of bacteriology JO - J Bacteriol VL - 201 IS - 19 N2 - Salmonella enterica serovar Typhimurium induces inflammatory diarrhea and bacterial uptake into intestinal epithelial cells using the Salmonella pathogenicity island 1 (SPI1) type III secretion system (T3SS). HilA activates transcription of the SPI1 structural components and effector proteins. Expression of hilA is activated by HilD, HilC, and RtsA, which act in a complex feed-forward regulatory loop. Many environmental signals and other regulators are integrated into this regulatory loop, primarily via HilD. After the invasion of Salmonella into host intestinal epithelial cells or during systemic replication in macrophages, the SPI T3SS is no longer required or expressed. We have shown that the two-component regulatory system PhoPQ, required for intracellular survival, represses the SPI1 T3SS mostly by controlling the transcription of hilA and hilD Here we show that PinT, one of the PhoPQ-regulated small RNAs (sRNAs), contributes to this regulation by repressing hilA and rtsA translation. PinT base pairs with both the hilA and rtsA mRNAs, resulting in translational inhibition of hilA, but also induces degradation of the rts transcript. PinT also indirectly represses expression of FliZ, a posttranslational regulator of HilD, and directly represses translation of ssrB, encoding the primary regulator of the SPI2 T3SS. Our in vivo mouse competition assays support the concept that PinT controls a series of virulence genes at the posttranscriptional level in order to adapt Salmonella from the invasion stage to intracellular survival.IMPORTANCESalmonella is one of the most important food-borne pathogens, infecting over one million people in the United States every year. These bacteria use a needle-like device to interact with intestinal epithelial cells, leading to invasion of the cells and induction of inflammatory diarrhea. A complex regulatory network controls expression of the invasion system in response to numerous environmental signals. Here we explore the molecular mechanisms by which the small RNA PinT contributes to this regulation, facilitating inactivation of the system after invasion. PinT controls several important virulence systems in Salmonella, tuning the transition between different stages of infection. SN - 1098-5530 UR - https://www.unboundmedicine.com/medline/citation/31262841/The_Small_RNA_PinT_Contributes_to_PhoP_Mediated_Regulation_of_the_Salmonella_Pathogenicity_Island_1_Type_III_Secretion_System_in_Salmonella_enterica_Serovar_Typhimurium_ DB - PRIME DP - Unbound Medicine ER -