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Expression and characterization of two chitinases with synergistic effect and antifungal activity from Xenorhabdus nematophila.
World J Microbiol Biotechnol. 2019 Jul 02; 35(7):106.WJ

Abstract

Xenorhabdus nematophila HB310 secreted the insecticidal protein toxin complex. Two chitinase genes, chi60 and chi70, were found in X. nematophila toxin complex locus. In order to clarify the function of two chitinases, chi60 and chi70 genes were cloned and expressed in Escherichia coli Transetta (DE3). As a result, we found that the Chi60 and Chi70 belonged to glycoside hydrolases (GH) family 18 with a molecular mass of 65 kDa and 78 kDa, respectively. When colloidal chitin was treated as the substrate, Chi60 and Chi70 were proved to have the highest enzymatic activity at pH 6.0 and 50 °C. Chi60 and Chi70 had obvious growth inhibition effect against the second larvae of Helicoverpa armigera with growth inhibiting rate of 81.99% and 90.51%. Chi70 had synergistic effect with the insecticidal toxicity of Bt Cry 1Ac, but the Chi60 had no synergistic effect with Bt Cry 1Ac. Chi60 and Chi70 showed antifungal activity against Alternaria brassicicola, Verticillium dahliae and Coniothyrium diplodiella. The results increased our understanding of the chitinases produced by X. nematophila and laid a foundation for further studies on the mechanism of the chitinases.

Authors+Show Affiliations

College of Plant Protection, Hebei Agricultural University, Baoding, 071001, Hebei, China.College of Plant Protection, Hebei Agricultural University, Baoding, 071001, Hebei, China.Luanping State-Owned Forestry Farm Management of Chengde City, Chengde, 068250, China.College of Plant Protection, Hebei Agricultural University, Baoding, 071001, Hebei, China.College of Plant Protection, Hebei Agricultural University, Baoding, 071001, Hebei, China.College of Plant Protection, Hebei Agricultural University, Baoding, 071001, Hebei, China.College of Plant Protection, Hebei Agricultural University, Baoding, 071001, Hebei, China. wqinying@hebau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31267229

Citation

Liu, Jia, et al. "Expression and Characterization of Two Chitinases With Synergistic Effect and Antifungal Activity From Xenorhabdus Nematophila." World Journal of Microbiology & Biotechnology, vol. 35, no. 7, 2019, p. 106.
Liu J, NanGong Z, Zhang J, et al. Expression and characterization of two chitinases with synergistic effect and antifungal activity from Xenorhabdus nematophila. World J Microbiol Biotechnol. 2019;35(7):106.
Liu, J., NanGong, Z., Zhang, J., Song, P., Tang, Y., Gao, Y., & Wang, Q. (2019). Expression and characterization of two chitinases with synergistic effect and antifungal activity from Xenorhabdus nematophila. World Journal of Microbiology & Biotechnology, 35(7), 106. https://doi.org/10.1007/s11274-019-2670-5
Liu J, et al. Expression and Characterization of Two Chitinases With Synergistic Effect and Antifungal Activity From Xenorhabdus Nematophila. World J Microbiol Biotechnol. 2019 Jul 2;35(7):106. PubMed PMID: 31267229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression and characterization of two chitinases with synergistic effect and antifungal activity from Xenorhabdus nematophila. AU - Liu,Jia, AU - NanGong,Ziyan, AU - Zhang,Jie, AU - Song,Ping, AU - Tang,Yin, AU - Gao,Yue, AU - Wang,Qinying, Y1 - 2019/07/02/ PY - 2019/03/22/received PY - 2019/06/05/accepted PY - 2019/7/4/entrez PY - 2019/7/4/pubmed PY - 2019/7/23/medline KW - Antifungal activity KW - Chitinases KW - Heterologous expression KW - Synergistic effect KW - Xenorhabdus nematophila SP - 106 EP - 106 JF - World journal of microbiology & biotechnology JO - World J Microbiol Biotechnol VL - 35 IS - 7 N2 - Xenorhabdus nematophila HB310 secreted the insecticidal protein toxin complex. Two chitinase genes, chi60 and chi70, were found in X. nematophila toxin complex locus. In order to clarify the function of two chitinases, chi60 and chi70 genes were cloned and expressed in Escherichia coli Transetta (DE3). As a result, we found that the Chi60 and Chi70 belonged to glycoside hydrolases (GH) family 18 with a molecular mass of 65 kDa and 78 kDa, respectively. When colloidal chitin was treated as the substrate, Chi60 and Chi70 were proved to have the highest enzymatic activity at pH 6.0 and 50 °C. Chi60 and Chi70 had obvious growth inhibition effect against the second larvae of Helicoverpa armigera with growth inhibiting rate of 81.99% and 90.51%. Chi70 had synergistic effect with the insecticidal toxicity of Bt Cry 1Ac, but the Chi60 had no synergistic effect with Bt Cry 1Ac. Chi60 and Chi70 showed antifungal activity against Alternaria brassicicola, Verticillium dahliae and Coniothyrium diplodiella. The results increased our understanding of the chitinases produced by X. nematophila and laid a foundation for further studies on the mechanism of the chitinases. SN - 1573-0972 UR - https://www.unboundmedicine.com/medline/citation/31267229/Expression_and_characterization_of_two_chitinases_with_synergistic_effect_and_antifungal_activity_from_Xenorhabdus_nematophila_ L2 - https://dx.doi.org/10.1007/s11274-019-2670-5 DB - PRIME DP - Unbound Medicine ER -