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LMM24 Encodes Receptor-Like Cytoplasmic Kinase 109, Which Regulates Cell Death and Defense Responses in Rice.
Int J Mol Sci. 2019 Jul 02; 20(13)IJ

Abstract

Lesion mimic mutants are excellent models for research on molecular mechanisms of cell death and defense responses in rice. We identified a new rice lesion mimic mutant lmm24 from a mutant pool of indica rice cultivar "ZhongHui8015". The LMM24 gene was identified by MutMap, and LMM24 was confirmed as a receptor-like cytoplasmic kinase 109 by amino acid sequence analysis. The lmm24 mutant displayed dark brown lesions in leaves and growth retardation that were not observed in wild-type ZH8015. The results of histochemical staining and TUNEL assays showed enhanced ROS accumulation and cell death in lmm24. Chloroplast degradation was observed in lmm24 leaves, with decreased expression of photosynthesis-related genes and increased expression of the senescence-induced STAYGREEN (SGR) gene and other senescence-associated genes. Furthermore, lmm24 exhibited enhanced resistance to rice blast fungus Magnaporthe oryzae (M. oryzae) and up-regulation of defense response genes. Our data demonstrate that LMM24 regulates cell death and defense responses in rice.

Authors+Show Affiliations

Key Laboratory for Zhejiang Super Rice Research and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.Key Laboratory for Zhejiang Super Rice Research and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.Key Laboratory for Zhejiang Super Rice Research and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.Key Laboratory for Zhejiang Super Rice Research and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.Key Laboratory for Zhejiang Super Rice Research and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.Key Laboratory for Zhejiang Super Rice Research and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.Key Laboratory for Zhejiang Super Rice Research and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.Key Laboratory for Zhejiang Super Rice Research and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. chengshihua@caas.cn.Key Laboratory for Zhejiang Super Rice Research and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. caoliyong@caas.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31269643

Citation

Zhang, Yue, et al. "LMM24 Encodes Receptor-Like Cytoplasmic Kinase 109, Which Regulates Cell Death and Defense Responses in Rice." International Journal of Molecular Sciences, vol. 20, no. 13, 2019.
Zhang Y, Liu Q, Zhang Y, et al. LMM24 Encodes Receptor-Like Cytoplasmic Kinase 109, Which Regulates Cell Death and Defense Responses in Rice. Int J Mol Sci. 2019;20(13).
Zhang, Y., Liu, Q., Zhang, Y., Chen, Y., Yu, N., Cao, Y., Zhan, X., Cheng, S., & Cao, L. (2019). LMM24 Encodes Receptor-Like Cytoplasmic Kinase 109, Which Regulates Cell Death and Defense Responses in Rice. International Journal of Molecular Sciences, 20(13). https://doi.org/10.3390/ijms20133243
Zhang Y, et al. LMM24 Encodes Receptor-Like Cytoplasmic Kinase 109, Which Regulates Cell Death and Defense Responses in Rice. Int J Mol Sci. 2019 Jul 2;20(13) PubMed PMID: 31269643.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LMM24 Encodes Receptor-Like Cytoplasmic Kinase 109, Which Regulates Cell Death and Defense Responses in Rice. AU - Zhang,Yue, AU - Liu,Qunen, AU - Zhang,Yingxin, AU - Chen,Yuyu, AU - Yu,Ning, AU - Cao,Yongrun, AU - Zhan,Xiaodeng, AU - Cheng,Shihua, AU - Cao,Liyong, Y1 - 2019/07/02/ PY - 2019/05/08/received PY - 2019/06/27/revised PY - 2019/07/01/accepted PY - 2019/7/5/entrez PY - 2019/7/5/pubmed PY - 2020/1/7/medline KW - defense responses KW - lesion mimic mutants (LMMs) KW - programmed cell death (PCD) KW - receptor-like cytoplasmic kinases (RLCKs) KW - rice (Oryza sativa L.) JF - International journal of molecular sciences JO - Int J Mol Sci VL - 20 IS - 13 N2 - Lesion mimic mutants are excellent models for research on molecular mechanisms of cell death and defense responses in rice. We identified a new rice lesion mimic mutant lmm24 from a mutant pool of indica rice cultivar "ZhongHui8015". The LMM24 gene was identified by MutMap, and LMM24 was confirmed as a receptor-like cytoplasmic kinase 109 by amino acid sequence analysis. The lmm24 mutant displayed dark brown lesions in leaves and growth retardation that were not observed in wild-type ZH8015. The results of histochemical staining and TUNEL assays showed enhanced ROS accumulation and cell death in lmm24. Chloroplast degradation was observed in lmm24 leaves, with decreased expression of photosynthesis-related genes and increased expression of the senescence-induced STAYGREEN (SGR) gene and other senescence-associated genes. Furthermore, lmm24 exhibited enhanced resistance to rice blast fungus Magnaporthe oryzae (M. oryzae) and up-regulation of defense response genes. Our data demonstrate that LMM24 regulates cell death and defense responses in rice. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/31269643/LMM24_Encodes_Receptor_Like_Cytoplasmic_Kinase_109_Which_Regulates_Cell_Death_and_Defense_Responses_in_Rice_ L2 - https://www.mdpi.com/resolver?pii=ijms20133243 DB - PRIME DP - Unbound Medicine ER -