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The differential effect of clarithromycin and azithromycin on induction of macrolide resistance in Mycobacterium abscessus.
Future Microbiol. 2019 06; 14:749-755.FM

Abstract

Aim:

Antibiotic resistance in Mycobacterium abscessus renders treatment poorly effective. Despite erm(41)-gene-mediated macrolide resistance, treatment with azithromycin or clarithromycin is recommended. It is contested whether macrolides differ in erm(41) induction. We determine whether this is the case.

Methods:

M. abscessus CIP104536 was used. Minimum inhibitory concentrations of clarithromycin and azithromycin were determined. Time-kill kinetics of M. abscessus exposed to azithromycin or clarithromycin were performed and RNA was isolated at predetermined intervals for erm(41) quantification.

Results:

Minimum inhibitory concentrations increased >30-fold. Time-kill kinetics showed a temporary bacteriostatic effect, abrogated by induced resistance. Erm(41) expression was increased following exposure to either macrolide for 7 days.

Conclusion:

Both macrolides induce resistance similarly, and this should not be an argument in choosing either macrolide for therapy.

Authors+Show Affiliations

Department of Medical Microbiology & Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Gelderland, The Netherlands.Department of Medical Microbiology & Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Gelderland, The Netherlands.Department of Medical Microbiology & Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Gelderland, The Netherlands.Department of Human Genetics, Donders Institute for Brain, Cognition & Behaviour, Radboud University Medical Center, Gelderland, The Netherlands.Department of Medical Microbiology & Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Gelderland, The Netherlands.Department of Pulmonary Diseases & Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Gelderland, The Netherlands.Department of Medical Microbiology & Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Gelderland, The Netherlands.Department of Medical Microbiology & Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Gelderland, The Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31271060

Citation

Schildkraut, Jodie A., et al. "The Differential Effect of Clarithromycin and Azithromycin On Induction of Macrolide Resistance in Mycobacterium Abscessus." Future Microbiology, vol. 14, 2019, pp. 749-755.
Schildkraut JA, Pennings LJ, Ruth MM, et al. The differential effect of clarithromycin and azithromycin on induction of macrolide resistance in Mycobacterium abscessus. Future Microbiol. 2019;14:749-755.
Schildkraut, J. A., Pennings, L. J., Ruth, M. M., de Brouwer, A. P., Wertheim, H. F., Hoefsloot, W., de Jong, A., & van Ingen, J. (2019). The differential effect of clarithromycin and azithromycin on induction of macrolide resistance in Mycobacterium abscessus. Future Microbiology, 14, 749-755. https://doi.org/10.2217/fmb-2018-0310
Schildkraut JA, et al. The Differential Effect of Clarithromycin and Azithromycin On Induction of Macrolide Resistance in Mycobacterium Abscessus. Future Microbiol. 2019;14:749-755. PubMed PMID: 31271060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The differential effect of clarithromycin and azithromycin on induction of macrolide resistance in Mycobacterium abscessus. AU - Schildkraut,Jodie A, AU - Pennings,Lian J, AU - Ruth,Mike M, AU - de Brouwer,Arjan Pm, AU - Wertheim,Heiman Fl, AU - Hoefsloot,Wouter, AU - de Jong,Arjan, AU - van Ingen,Jakko, Y1 - 2019/07/04/ PY - 2019/7/5/pubmed PY - 2020/1/24/medline PY - 2019/7/5/entrez KW - KW - azithromycin KW - clarithromycin KW - macrolide resistance SP - 749 EP - 755 JF - Future microbiology JO - Future Microbiol VL - 14 N2 - Aim: Antibiotic resistance in Mycobacterium abscessus renders treatment poorly effective. Despite erm(41)-gene-mediated macrolide resistance, treatment with azithromycin or clarithromycin is recommended. It is contested whether macrolides differ in erm(41) induction. We determine whether this is the case. Methods: M. abscessus CIP104536 was used. Minimum inhibitory concentrations of clarithromycin and azithromycin were determined. Time-kill kinetics of M. abscessus exposed to azithromycin or clarithromycin were performed and RNA was isolated at predetermined intervals for erm(41) quantification. Results: Minimum inhibitory concentrations increased >30-fold. Time-kill kinetics showed a temporary bacteriostatic effect, abrogated by induced resistance. Erm(41) expression was increased following exposure to either macrolide for 7 days. Conclusion: Both macrolides induce resistance similarly, and this should not be an argument in choosing either macrolide for therapy. SN - 1746-0921 UR - https://www.unboundmedicine.com/medline/citation/31271060/The_differential_effect_of_clarithromycin_and_azithromycin_on_induction_of_macrolide_resistance_in_Mycobacterium_abscessus_ L2 - https://www.futuremedicine.com/doi/10.2217/fmb-2018-0310?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -