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Effects of probiotic supplementation of a plant-based protein diet on intestinal microbial diversity, digestive enzyme activity, intestinal structure, and immunity in olive flounder (Paralichthys olivaceus).
Fish Shellfish Immunol. 2019 Sep; 92:719-727.FS

Abstract

The aim of this study was to investigate the effects of intestinal microbial manipulation by dietary probiotic supplementation on digestive enzyme activity, immune-related gene transcription, intestinal structure alteration, and viability against pathogenic challenge in olive flounder. Similar-sized flounders (14.92 ± 0.21 g) were divided into three groups and supplemented with a control (without probiotic) or 1 × 108 CFU/g diet of each of Bacillus sp. SJ-10 (ProB) and Lactobacillus plantarum (ProL) for eight weeks. At the end of the feeding trial, the estimated intestinal microbial richness (Chao1) and diversity (Shannon) demonstrated a significant (P < 0.05) abundance in the ProB group (484.80 ± 88.75, 5.08 ± 0.17) compared to the ProL (285.32 ± 17.78, 4.54 ± 0.09) and control groups (263.23 ± 20.20, 4.30 ± 0.20). A similar alteration phenomenon was also found at the phylum level, with a higher abundance of Proteobacteria, Actinobacteria, and Acidobacteria. Trypsin and lipase activities were elevated in both the ProB and ProL groups compared to the control, but amylase was only higher in the ProB group. The expression levels of pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 were significantly higher in the ProB group than in the other two groups. There was a significant increase in transcription of IL-10 in both the ProB and ProL groups compared to the control. The length of villi and microvilli of probiotic-fed olive flounder was increased but was not significantly different from the control group. In an in vivo challenge experiment with Streptococcus iniae (1 × 108 CFU/mL), the survival rates of the ProB and ProL groups were 29.17% and 12.50%, respectively, when control mortality reached 100%. Therefore, intestinal microbiota manipulation by probiotic supplementation increased the richness of the bacterial population, digestive enzyme activity, intestinal immune gene transcription, and infectious disease protection in olive flounder.

Authors+Show Affiliations

Department of Biotechnology, Pukyong National University, Busan, 48513, Republic of Korea.Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.Department of Biotechnology, Pukyong National University, Busan, 48513, Republic of Korea; Department of Aquaculture, Sylhet Agricultural University, Sylhet, 3100, Bangladesh.Aquafeed Research Center, NIFS, Pohang, 791-923, Republic of Korea.Aquafeed Research Center, NIFS, Pohang, 791-923, Republic of Korea.Department of Biotechnology, Pukyong National University, Busan, 48513, Republic of Korea. Electronic address: iskong@pknu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31271838

Citation

Jang, Won Je, et al. "Effects of Probiotic Supplementation of a Plant-based Protein Diet On Intestinal Microbial Diversity, Digestive Enzyme Activity, Intestinal Structure, and Immunity in Olive Flounder (Paralichthys Olivaceus)." Fish & Shellfish Immunology, vol. 92, 2019, pp. 719-727.
Jang WJ, Lee JM, Hasan MT, et al. Effects of probiotic supplementation of a plant-based protein diet on intestinal microbial diversity, digestive enzyme activity, intestinal structure, and immunity in olive flounder (Paralichthys olivaceus). Fish Shellfish Immunol. 2019;92:719-727.
Jang, W. J., Lee, J. M., Hasan, M. T., Lee, B. J., Lim, S. G., & Kong, I. S. (2019). Effects of probiotic supplementation of a plant-based protein diet on intestinal microbial diversity, digestive enzyme activity, intestinal structure, and immunity in olive flounder (Paralichthys olivaceus). Fish & Shellfish Immunology, 92, 719-727. https://doi.org/10.1016/j.fsi.2019.06.056
Jang WJ, et al. Effects of Probiotic Supplementation of a Plant-based Protein Diet On Intestinal Microbial Diversity, Digestive Enzyme Activity, Intestinal Structure, and Immunity in Olive Flounder (Paralichthys Olivaceus). Fish Shellfish Immunol. 2019;92:719-727. PubMed PMID: 31271838.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of probiotic supplementation of a plant-based protein diet on intestinal microbial diversity, digestive enzyme activity, intestinal structure, and immunity in olive flounder (Paralichthys olivaceus). AU - Jang,Won Je, AU - Lee,Jong Min, AU - Hasan,Md Tawheed, AU - Lee,Bong-Joo, AU - Lim,Sang Gu, AU - Kong,In-Soo, Y1 - 2019/07/02/ PY - 2019/04/04/received PY - 2019/06/21/revised PY - 2019/06/29/accepted PY - 2019/7/5/pubmed PY - 2019/11/23/medline PY - 2019/7/5/entrez KW - Digestive enzyme KW - Immunity KW - Microbiota KW - Olive flounder KW - Probiotic SP - 719 EP - 727 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 92 N2 - The aim of this study was to investigate the effects of intestinal microbial manipulation by dietary probiotic supplementation on digestive enzyme activity, immune-related gene transcription, intestinal structure alteration, and viability against pathogenic challenge in olive flounder. Similar-sized flounders (14.92 ± 0.21 g) were divided into three groups and supplemented with a control (without probiotic) or 1 × 108 CFU/g diet of each of Bacillus sp. SJ-10 (ProB) and Lactobacillus plantarum (ProL) for eight weeks. At the end of the feeding trial, the estimated intestinal microbial richness (Chao1) and diversity (Shannon) demonstrated a significant (P < 0.05) abundance in the ProB group (484.80 ± 88.75, 5.08 ± 0.17) compared to the ProL (285.32 ± 17.78, 4.54 ± 0.09) and control groups (263.23 ± 20.20, 4.30 ± 0.20). A similar alteration phenomenon was also found at the phylum level, with a higher abundance of Proteobacteria, Actinobacteria, and Acidobacteria. Trypsin and lipase activities were elevated in both the ProB and ProL groups compared to the control, but amylase was only higher in the ProB group. The expression levels of pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 were significantly higher in the ProB group than in the other two groups. There was a significant increase in transcription of IL-10 in both the ProB and ProL groups compared to the control. The length of villi and microvilli of probiotic-fed olive flounder was increased but was not significantly different from the control group. In an in vivo challenge experiment with Streptococcus iniae (1 × 108 CFU/mL), the survival rates of the ProB and ProL groups were 29.17% and 12.50%, respectively, when control mortality reached 100%. Therefore, intestinal microbiota manipulation by probiotic supplementation increased the richness of the bacterial population, digestive enzyme activity, intestinal immune gene transcription, and infectious disease protection in olive flounder. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/31271838/Effects_of_probiotic_supplementation_of_a_plant_based_protein_diet_on_intestinal_microbial_diversity_digestive_enzyme_activity_intestinal_structure_and_immunity_in_olive_flounder__Paralichthys_olivaceus__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(19)30703-X DB - PRIME DP - Unbound Medicine ER -