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Mechanical impact of epithelial-mesenchymal transition on epithelial morphogenesis in Drosophila.
Nat Commun 2019; 10(1):2951NC

Abstract

Epithelial-mesenchymal transition (EMT) is an essential process both in physiological and pathological contexts. Intriguingly, EMT is often associated with tissue invagination during development; however, the impact of EMT on tissue remodeling remain unexplored. Here, we show that at the initiation of the EMT process, cells produce an apico-basal force, orthogonal to the surface of the epithelium, that constitutes an important driving force for tissue invagination in Drosophila. When EMT is ectopically induced, cells starting their delamination generate an orthogonal force and induce ectopic folding. Similarly, during mesoderm invagination, cells undergoing EMT generate an apico-basal force through the formation of apico-basal structures of myosin II. Using both laser microdissection and in silico physical modelling, we show that mesoderm invagination does not proceed if apico-basal forces are impaired, indicating that they constitute driving forces in the folding process. Altogether, these data reveal the mechanical impact of EMT on morphogenesis.

Authors+Show Affiliations

LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, Toulouse, 31062, France.LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, Toulouse, 31062, France. Morphogénie Logiciels, 32110, St Martin d'Armagnac, France.LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, Toulouse, 31062, France.Morphogénie Logiciels, 32110, St Martin d'Armagnac, France. guillaume@damcb.com.LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, Toulouse, 31062, France. corinne.ben-assayag@univ-tlse3.fr.LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, Toulouse, 31062, France. magali.suzanne@univ-tlse3.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31273212

Citation

Gracia, Mélanie, et al. "Mechanical Impact of Epithelial-mesenchymal Transition On Epithelial Morphogenesis in Drosophila." Nature Communications, vol. 10, no. 1, 2019, p. 2951.
Gracia M, Theis S, Proag A, et al. Mechanical impact of epithelial-mesenchymal transition on epithelial morphogenesis in Drosophila. Nat Commun. 2019;10(1):2951.
Gracia, M., Theis, S., Proag, A., Gay, G., Benassayag, C., & Suzanne, M. (2019). Mechanical impact of epithelial-mesenchymal transition on epithelial morphogenesis in Drosophila. Nature Communications, 10(1), p. 2951. doi:10.1038/s41467-019-10720-0.
Gracia M, et al. Mechanical Impact of Epithelial-mesenchymal Transition On Epithelial Morphogenesis in Drosophila. Nat Commun. 2019 Jul 4;10(1):2951. PubMed PMID: 31273212.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanical impact of epithelial-mesenchymal transition on epithelial morphogenesis in Drosophila. AU - Gracia,Mélanie, AU - Theis,Sophie, AU - Proag,Amsha, AU - Gay,Guillaume, AU - Benassayag,Corinne, AU - Suzanne,Magali, Y1 - 2019/07/04/ PY - 2018/11/07/received PY - 2019/05/28/accepted PY - 2019/7/6/entrez PY - 2019/7/6/pubmed PY - 2019/7/6/medline SP - 2951 EP - 2951 JF - Nature communications JO - Nat Commun VL - 10 IS - 1 N2 - Epithelial-mesenchymal transition (EMT) is an essential process both in physiological and pathological contexts. Intriguingly, EMT is often associated with tissue invagination during development; however, the impact of EMT on tissue remodeling remain unexplored. Here, we show that at the initiation of the EMT process, cells produce an apico-basal force, orthogonal to the surface of the epithelium, that constitutes an important driving force for tissue invagination in Drosophila. When EMT is ectopically induced, cells starting their delamination generate an orthogonal force and induce ectopic folding. Similarly, during mesoderm invagination, cells undergoing EMT generate an apico-basal force through the formation of apico-basal structures of myosin II. Using both laser microdissection and in silico physical modelling, we show that mesoderm invagination does not proceed if apico-basal forces are impaired, indicating that they constitute driving forces in the folding process. Altogether, these data reveal the mechanical impact of EMT on morphogenesis. SN - 2041-1723 UR - https://www.unboundmedicine.com/medline/citation/31273212/Mechanical_impact_of_epithelial-mesenchymal_transition_on_epithelial_morphogenesis_in_Drosophila L2 - http://dx.doi.org/10.1038/s41467-019-10720-0 DB - PRIME DP - Unbound Medicine ER -