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Identification of P genome chromosomes in Agropyron cristatum and wheat-A. cristatum derivative lines by FISH.
Sci Rep. 2019 07 04; 9(1):9712.SR

Abstract

Agropyron cristatum (L.) Gaertn. (P genome) is cultivated as pasture fodder and can provide many desirable genes for wheat improvement. With the development of genomics and fluorescence in situ hybridization (FISH) technology, probes for identifying plant chromosomes were also developed. However, there are few reports on A. cristatum chromosomes. Here, FISH with the repeated sequences pAcTRT1 and pAcpCR2 enabled the identification of all diploid A. cristatum chromosomes. An integrated idiogram of A. cristatum chromosomes was constructed based on the FISH patterns of five diploid A. cristatum individuals. Structural polymorphisms of homologous chromosomes were observed not only among different individuals but also within individuals. Moreover, seventeen wheat-A. cristatum introgression lines containing different P genome chromosomes were identified with pAcTRT1 and pAcpCR2 probes. The arrangement of chromosomes in diploid A. cristatum was determined by identifying correspondence between the P chromosomes in these genetically identified introgression lines and diploid A. cristatum chromosomes. The two probes were also effective for discriminating all chromosomes of tetraploid A. cristatum, and the differences between two tetraploid A. cristatum accessions were similar to the polymorphisms among individuals of diploid A. cristatum. Collectively, the results provide an effective means for chromosome identification and phylogenetic studies of P genome chromosomes.

Authors+Show Affiliations

National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. lilihui@caas.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31273296

Citation

Han, Haiming, et al. "Identification of P Genome Chromosomes in Agropyron Cristatum and wheat-A. Cristatum Derivative Lines By FISH." Scientific Reports, vol. 9, no. 1, 2019, p. 9712.
Han H, Liu W, Zhang J, et al. Identification of P genome chromosomes in Agropyron cristatum and wheat-A. cristatum derivative lines by FISH. Sci Rep. 2019;9(1):9712.
Han, H., Liu, W., Zhang, J., Zhou, S., Yang, X., Li, X., & Li, L. (2019). Identification of P genome chromosomes in Agropyron cristatum and wheat-A. cristatum derivative lines by FISH. Scientific Reports, 9(1), 9712. https://doi.org/10.1038/s41598-019-46197-6
Han H, et al. Identification of P Genome Chromosomes in Agropyron Cristatum and wheat-A. Cristatum Derivative Lines By FISH. Sci Rep. 2019 07 4;9(1):9712. PubMed PMID: 31273296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of P genome chromosomes in Agropyron cristatum and wheat-A. cristatum derivative lines by FISH. AU - Han,Haiming, AU - Liu,Weihua, AU - Zhang,Jinpeng, AU - Zhou,Shenghui, AU - Yang,Xinming, AU - Li,Xiuquan, AU - Li,Lihui, Y1 - 2019/07/04/ PY - 2019/03/15/received PY - 2019/06/24/accepted PY - 2019/7/6/entrez PY - 2019/7/6/pubmed PY - 2020/10/27/medline SP - 9712 EP - 9712 JF - Scientific reports JO - Sci Rep VL - 9 IS - 1 N2 - Agropyron cristatum (L.) Gaertn. (P genome) is cultivated as pasture fodder and can provide many desirable genes for wheat improvement. With the development of genomics and fluorescence in situ hybridization (FISH) technology, probes for identifying plant chromosomes were also developed. However, there are few reports on A. cristatum chromosomes. Here, FISH with the repeated sequences pAcTRT1 and pAcpCR2 enabled the identification of all diploid A. cristatum chromosomes. An integrated idiogram of A. cristatum chromosomes was constructed based on the FISH patterns of five diploid A. cristatum individuals. Structural polymorphisms of homologous chromosomes were observed not only among different individuals but also within individuals. Moreover, seventeen wheat-A. cristatum introgression lines containing different P genome chromosomes were identified with pAcTRT1 and pAcpCR2 probes. The arrangement of chromosomes in diploid A. cristatum was determined by identifying correspondence between the P chromosomes in these genetically identified introgression lines and diploid A. cristatum chromosomes. The two probes were also effective for discriminating all chromosomes of tetraploid A. cristatum, and the differences between two tetraploid A. cristatum accessions were similar to the polymorphisms among individuals of diploid A. cristatum. Collectively, the results provide an effective means for chromosome identification and phylogenetic studies of P genome chromosomes. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/31273296/Identification_of_P_genome_chromosomes_in_Agropyron_cristatum_and_wheat_A__cristatum_derivative_lines_by_FISH_ L2 - https://doi.org/10.1038/s41598-019-46197-6 DB - PRIME DP - Unbound Medicine ER -