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Development of a Cranberry Standard for Quantification of Insoluble Cranberry (Vaccinium macrocarpon Ait.) Proanthocyanidins.
J Agric Food Chem. 2020 Mar 11; 68(10):2900-2905.JA

Abstract

Cranberry proanthocyanidins (PACs) can be partitioned into soluble PACs, which are extracted with solvents, and insoluble PACs, which remain associated with fibers and proteins after extraction. Most research on cranberry products only quantifies soluble PACs because proper standards for quantifying insoluble PACs are lacking. In this study, we evaluated the ability of a cranberry PAC (c-PAC) standard, reflective of the structural heterogeneity of PACs found in cranberry fruit, to quantify insoluble PACs by the butanol-hydrochloric acid (BuOH-HCl) method. For the first time, a c-PAC standard enabled conversion of BuOH-HCl absorbance values (550 nm) to a weight (milligram) basis, allowing for quantification of insoluble PACs in cranberries. The use of the c-PAC reference standard for sequential analysis of soluble PACs by the method of 4-(dimethylamino)cinnamaldehyde and insoluble PACs by the method of BuOH-HCl provides analytical tools for the standardization of cranberry-based ingredients.

Authors+Show Affiliations

Reed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, Wisconsin 53706, United States.Reed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, Wisconsin 53706, United States. Complete Phytochemical Solutions LLC, 317 South Street, Cambridge, Wisconsin 53523, United States.Complete Phytochemical Solutions LLC, 317 South Street, Cambridge, Wisconsin 53523, United States.Complete Phytochemical Solutions LLC, 317 South Street, Cambridge, Wisconsin 53523, United States.Reed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, Wisconsin 53706, United States. Complete Phytochemical Solutions LLC, 317 South Street, Cambridge, Wisconsin 53523, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31274305

Citation

Gullickson, Erica R., et al. "Development of a Cranberry Standard for Quantification of Insoluble Cranberry (Vaccinium Macrocarpon Ait.) Proanthocyanidins." Journal of Agricultural and Food Chemistry, vol. 68, no. 10, 2020, pp. 2900-2905.
Gullickson ER, Krueger CG, Birmingham A, et al. Development of a Cranberry Standard for Quantification of Insoluble Cranberry (Vaccinium macrocarpon Ait.) Proanthocyanidins. J Agric Food Chem. 2020;68(10):2900-2905.
Gullickson, E. R., Krueger, C. G., Birmingham, A., Maranan, M., & Reed, J. D. (2020). Development of a Cranberry Standard for Quantification of Insoluble Cranberry (Vaccinium macrocarpon Ait.) Proanthocyanidins. Journal of Agricultural and Food Chemistry, 68(10), 2900-2905. https://doi.org/10.1021/acs.jafc.9b03696
Gullickson ER, et al. Development of a Cranberry Standard for Quantification of Insoluble Cranberry (Vaccinium Macrocarpon Ait.) Proanthocyanidins. J Agric Food Chem. 2020 Mar 11;68(10):2900-2905. PubMed PMID: 31274305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a Cranberry Standard for Quantification of Insoluble Cranberry (Vaccinium macrocarpon Ait.) Proanthocyanidins. AU - Gullickson,Erica R, AU - Krueger,Christian G, AU - Birmingham,Andrew, AU - Maranan,Michael, AU - Reed,Jess D, Y1 - 2019/07/22/ PY - 2019/7/6/pubmed PY - 2020/3/21/medline PY - 2019/7/6/entrez KW - butanol−HCl KW - cranberry KW - insoluble proanthocyanidin SP - 2900 EP - 2905 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 68 IS - 10 N2 - Cranberry proanthocyanidins (PACs) can be partitioned into soluble PACs, which are extracted with solvents, and insoluble PACs, which remain associated with fibers and proteins after extraction. Most research on cranberry products only quantifies soluble PACs because proper standards for quantifying insoluble PACs are lacking. In this study, we evaluated the ability of a cranberry PAC (c-PAC) standard, reflective of the structural heterogeneity of PACs found in cranberry fruit, to quantify insoluble PACs by the butanol-hydrochloric acid (BuOH-HCl) method. For the first time, a c-PAC standard enabled conversion of BuOH-HCl absorbance values (550 nm) to a weight (milligram) basis, allowing for quantification of insoluble PACs in cranberries. The use of the c-PAC reference standard for sequential analysis of soluble PACs by the method of 4-(dimethylamino)cinnamaldehyde and insoluble PACs by the method of BuOH-HCl provides analytical tools for the standardization of cranberry-based ingredients. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/31274305/Development_of_a_Cranberry_Standard_for_Quantification_of_Insoluble_Cranberry__Vaccinium_macrocarpon_Ait___Proanthocyanidins_ L2 - https://dx.doi.org/10.1021/acs.jafc.9b03696 DB - PRIME DP - Unbound Medicine ER -