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Hard and soft X-ray imaging to resolve human ovarian cortical structures.
J Synchrotron Radiat 2019; 26(Pt 4):1322-1329JS

Abstract

Laboratory and synchrotron X-ray tomography are powerful tools for non-invasive studies of biological samples at micrometric resolution. In particular, the development of phase contrast imaging is enabling the visualization of sample details with a small range of attenuation coefficients, thus allowing in-depth analyses of anatomical and histological structures. Reproductive medicine is starting to profit from these techniques, mainly applied to animal models. This study reports the first imaging of human ovarian tissue where the samples consisted of surgically obtained millimetre fragments, properly fixed, stained with osmium tetroxide and included in epoxydic resin. Samples were imaged by the use of propagation phase contrast synchrotron radiation micro-computed tomography (microCT), obtained at the SYRMEP beamline of Elettra light source (Trieste, Italy), and X-ray absorption microCT at the Theoretical Biology MicroCT Imaging Laboratory in Vienna, Austria. The reconstructed microCT images were compared with the soft X-ray absorption and phase contrast images acquired at the TwinMic beamline of Elettra in order to help with the identification of structures. The resulting images allow the regions of the cortex and medulla of the ovary to be distinguished, identifying early-stage follicles and visualizing the distribution of blood vessels. The study opens to further application of micro-resolved 3D imaging to improve the understanding of human ovary's structure and support diagnostics as well as advances in reproductive technologies.

Authors+Show Affiliations

Institute for Maternal and Child Health, IRCCS Burlo Garofolo, 34147 Trieste, Italy.Nuclear Engineering Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-594, Brazil.Elettra Sincrotrone Trieste, SS 14 km 163.5 in Area Science Park, Basovizza, 34149 Trieste, Italy.Department of Medical, Surgical and Health Sciences, University of Trieste, 34149 Trieste, Italy.Elettra Sincrotrone Trieste, SS 14 km 163.5 in Area Science Park, Basovizza, 34149 Trieste, Italy.Department of Theoretical Biology, University of Vienna, Vienna, Austria.Institute for Maternal and Child Health, IRCCS Burlo Garofolo, 34147 Trieste, Italy.Institute for Maternal and Child Health, IRCCS Burlo Garofolo, 34147 Trieste, Italy.Elettra Sincrotrone Trieste, SS 14 km 163.5 in Area Science Park, Basovizza, 34149 Trieste, Italy.Physics Institute, State University of Rio de Janeiro (UERJ), Rio de Janeiro 21941-594, Brazil.Elettra Sincrotrone Trieste, SS 14 km 163.5 in Area Science Park, Basovizza, 34149 Trieste, Italy.Department of Medical, Surgical and Health Sciences, University of Trieste, 34149 Trieste, Italy.Institute for Maternal and Child Health, IRCCS Burlo Garofolo, 34147 Trieste, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31274461

Citation

Pascolo, Lorella, et al. "Hard and Soft X-ray Imaging to Resolve Human Ovarian Cortical Structures." Journal of Synchrotron Radiation, vol. 26, no. Pt 4, 2019, pp. 1322-1329.
Pascolo L, Sena G, Gianoncelli A, et al. Hard and soft X-ray imaging to resolve human ovarian cortical structures. J Synchrotron Radiat. 2019;26(Pt 4):1322-1329.
Pascolo, L., Sena, G., Gianoncelli, A., Cernogoraz, A., Kourousias, G., Metscher, B. D., ... Ricci, G. (2019). Hard and soft X-ray imaging to resolve human ovarian cortical structures. Journal of Synchrotron Radiation, 26(Pt 4), pp. 1322-1329. doi:10.1107/S1600577519003680.
Pascolo L, et al. Hard and Soft X-ray Imaging to Resolve Human Ovarian Cortical Structures. J Synchrotron Radiat. 2019 Jul 1;26(Pt 4):1322-1329. PubMed PMID: 31274461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hard and soft X-ray imaging to resolve human ovarian cortical structures. AU - Pascolo,Lorella, AU - Sena,Gabriela, AU - Gianoncelli,Alessandra, AU - Cernogoraz,Alice, AU - Kourousias,Geroge, AU - Metscher,Brian D, AU - Romano,Federico, AU - Zito,Gabriella, AU - Pacilè,Serena, AU - Barroso,Regina, AU - Tromba,Giuliana, AU - Zweyer,Marina, AU - Ricci,Giuseppe, Y1 - 2019/05/14/ PY - 2018/12/19/received PY - 2019/03/15/accepted PY - 2019/7/6/entrez KW - STXM KW - microtomography KW - ovary KW - phase contrast tomography SP - 1322 EP - 1329 JF - Journal of synchrotron radiation JO - J Synchrotron Radiat VL - 26 IS - Pt 4 N2 - Laboratory and synchrotron X-ray tomography are powerful tools for non-invasive studies of biological samples at micrometric resolution. In particular, the development of phase contrast imaging is enabling the visualization of sample details with a small range of attenuation coefficients, thus allowing in-depth analyses of anatomical and histological structures. Reproductive medicine is starting to profit from these techniques, mainly applied to animal models. This study reports the first imaging of human ovarian tissue where the samples consisted of surgically obtained millimetre fragments, properly fixed, stained with osmium tetroxide and included in epoxydic resin. Samples were imaged by the use of propagation phase contrast synchrotron radiation micro-computed tomography (microCT), obtained at the SYRMEP beamline of Elettra light source (Trieste, Italy), and X-ray absorption microCT at the Theoretical Biology MicroCT Imaging Laboratory in Vienna, Austria. The reconstructed microCT images were compared with the soft X-ray absorption and phase contrast images acquired at the TwinMic beamline of Elettra in order to help with the identification of structures. The resulting images allow the regions of the cortex and medulla of the ovary to be distinguished, identifying early-stage follicles and visualizing the distribution of blood vessels. The study opens to further application of micro-resolved 3D imaging to improve the understanding of human ovary's structure and support diagnostics as well as advances in reproductive technologies. SN - 1600-5775 UR - https://www.unboundmedicine.com/medline/citation/31274461/Hard_and_soft_X-ray_imaging_to_resolve_human_ovarian_cortical_structures L2 - http://scripts.iucr.org/cgi-bin/paper?S1600577519003680 DB - PRIME DP - Unbound Medicine ER -