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Hepatoprotective effect of Lobelia alsinoides Lam. in Wistar rats.

Abstract

BACKGROUND

Traditional healing practitioners of South India use fine paste (an Ayurvedic dosage form known as 'kalka') of Lobelia alsinoides Lam., an ethno medicinal plant for curing hepatic diseases.

OBJECTIVE

To evaluate in-vivo hepatoprotective effect of a candidate formulation viz. kalka containing whole plant (L. alsinoides Lam.) in rat model of Carbon-tetrachloride (CCl4) induced hepatotoxicity.

MATERIALS & METHODS

Hepatotoxicity was induced in Wistar albino rats by oral administration of 1.25 ml/kg CCl4 once every day for 7 consecutive days. A candidate kalka formulation (fine paste) was prepared and administered to rats at different dose rates of 0.54 g/kg, 1.08 g/kg and 2.16 g/kg daily. At the end of the study-period, the serum levels of aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin, total protein, albumin and total cholesterol were monitored. Further, the hepatic pathology was evaluated for assessing the extent of hepatotoxicity in the control and hepatoprotective effect in treatment groups. Meanwhile in-vitro antioxidant activity of kalka was evaluated by hydroxy radical, nitric oxide and DPPH (2, 2 diphenyl-1-picrylhydrazil) radical scavenging assays. Further, a 'limit test' was done in accordance with OECD Guidelines 425 (acute toxicity).

RESULTS

The animals treated with the fine paste of L. alsinoides did not show an elevation in the biochemical values compared to CCl4 treated rats and during histomorphologic evaluation, hepatoprotective effect was evident with scattered mitotic figures in the parenchyma. Acute toxicity evaluation indicated that doses up to 2500 mg/kg are not toxic to rats. It has a good anti-oxidant activity also.

CONCLUSIONS

From the study, it was obvious that L. alsinoides had significant hepatoprotective effect in CCl4 induced liver toxicity in rats. This ethno medicinal plant is certainly a promising hepatoprotective drug in liver disorders.

Authors+Show Affiliations

Drug Standardisation Unit, Govt. Ayurveda College, Thiruvananthapuram, Kerala, India. Electronic address: drbinithasreejith@gmail.com.Dept. of Dravyagunavijnanam, Govt. Ayurveda College, Thiruvananthapuram, Kerala, India.Regional Occupational Health Centre (Southern), ICMR Complex, Kannamangala PO, Poojanahalli Road, Devanahalli Thaluk, Bangaluru 562110, Karnataka, India.Division of Experimental Pathology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695012, India.Drug Standardisation Unit, Govt. Ayurveda College, Thiruvananthapuram, Kerala, India.Dept. of Dravyagunavijnanam, Govt. Ayurveda College, Thiruvananthapuram, Kerala, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31277907

Citation

Binitha, Raj R V., et al. "Hepatoprotective Effect of Lobelia Alsinoides Lam. in Wistar Rats." Journal of Ayurveda and Integrative Medicine, 2019.
Binitha RRV, Shajahan MA, Muhamed J, et al. Hepatoprotective effect of Lobelia alsinoides Lam. in Wistar rats. J Ayurveda Integr Med. 2019.
Binitha, R. R. V., Shajahan, M. A., Muhamed, J., Anilkumar, T. V., Premlal, S., & Indulekha, V. C. (2019). Hepatoprotective effect of Lobelia alsinoides Lam. in Wistar rats. Journal of Ayurveda and Integrative Medicine, doi:10.1016/j.jaim.2019.04.004.
Binitha RRV, et al. Hepatoprotective Effect of Lobelia Alsinoides Lam. in Wistar Rats. J Ayurveda Integr Med. 2019 Jul 2; PubMed PMID: 31277907.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective effect of Lobelia alsinoides Lam. in Wistar rats. AU - Binitha,Raj R V, AU - Shajahan,M A, AU - Muhamed,Jaseer, AU - Anilkumar,Thapasimuthu V, AU - Premlal,S, AU - Indulekha,V C, Y1 - 2019/07/02/ PY - 2018/05/17/received PY - 2019/02/15/revised PY - 2019/04/01/accepted PY - 2019/7/7/entrez KW - Ayurveda KW - Lobelia alsinoides Lam. KW - kalka JF - Journal of Ayurveda and integrative medicine JO - J Ayurveda Integr Med N2 - BACKGROUND: Traditional healing practitioners of South India use fine paste (an Ayurvedic dosage form known as 'kalka') of Lobelia alsinoides Lam., an ethno medicinal plant for curing hepatic diseases. OBJECTIVE: To evaluate in-vivo hepatoprotective effect of a candidate formulation viz. kalka containing whole plant (L. alsinoides Lam.) in rat model of Carbon-tetrachloride (CCl4) induced hepatotoxicity. MATERIALS & METHODS: Hepatotoxicity was induced in Wistar albino rats by oral administration of 1.25 ml/kg CCl4 once every day for 7 consecutive days. A candidate kalka formulation (fine paste) was prepared and administered to rats at different dose rates of 0.54 g/kg, 1.08 g/kg and 2.16 g/kg daily. At the end of the study-period, the serum levels of aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin, total protein, albumin and total cholesterol were monitored. Further, the hepatic pathology was evaluated for assessing the extent of hepatotoxicity in the control and hepatoprotective effect in treatment groups. Meanwhile in-vitro antioxidant activity of kalka was evaluated by hydroxy radical, nitric oxide and DPPH (2, 2 diphenyl-1-picrylhydrazil) radical scavenging assays. Further, a 'limit test' was done in accordance with OECD Guidelines 425 (acute toxicity). RESULTS: The animals treated with the fine paste of L. alsinoides did not show an elevation in the biochemical values compared to CCl4 treated rats and during histomorphologic evaluation, hepatoprotective effect was evident with scattered mitotic figures in the parenchyma. Acute toxicity evaluation indicated that doses up to 2500 mg/kg are not toxic to rats. It has a good anti-oxidant activity also. CONCLUSIONS: From the study, it was obvious that L. alsinoides had significant hepatoprotective effect in CCl4 induced liver toxicity in rats. This ethno medicinal plant is certainly a promising hepatoprotective drug in liver disorders. SN - 0975-9476 UR - https://www.unboundmedicine.com/medline/citation/31277907/Hepatoprotective_effect_of_Lobelia_alsinoides_Lam._in_Wistar_rats L2 - https://linkinghub.elsevier.com/retrieve/pii/S0975-9476(18)30454-6 DB - PRIME DP - Unbound Medicine ER -