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Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments.
Sci Rep 2019; 9(1):9725SR

Abstract

Sedimentary biofilms comprising microbial communities mediating the anaerobic oxidation of methane are rare. Here, we describe two biofilm communities discovered in sediment cores recovered from Arctic cold seep sites (gas hydrate pingos) in the north-western Barents Sea, characterized by steady methane fluxes. We found macroscopically visible biofilms in pockets in the sediment matrix at the depth of the sulphate-methane-transition zone. 16S rRNA gene surveys revealed that the microbial community in one of the two biofilms comprised exclusively of putative anaerobic methanotrophic archaea of which ANME-1 was the sole archaeal taxon. The bacterial community consisted of relatives of sulphate-reducing bacteria (SRB) belonging to uncultured Desulfobacteraceae clustering into SEEP-SRB1 (i.e. the typical SRB associated to ANME-1), and members of the atribacterial JS1 clade. Confocal laser scanning microscopy demonstrates that this biofilm is composed of multicellular strands and patches of ANME-1 that are loosely associated with SRB cells, but not tightly connected in aggregates. Our discovery of methanotrophic biofilms in sediment pockets closely associated with methane seeps constitutes a hitherto overlooked and potentially widespread sink for methane and sulphate in marine sediments.

Authors+Show Affiliations

CAGE - Centre for Arctic Gas Hydrate, Environment and Climate, Department of Geosciences, UiT The Arctic University of Norway, Tromsø, Norway. friederike.gruendger@bios.au.dk.CAGE - Centre for Arctic Gas Hydrate, Environment and Climate, Department of Geosciences, UiT The Arctic University of Norway, Tromsø, Norway.CAGE - Centre for Arctic Gas Hydrate, Environment and Climate, Department of Geosciences, UiT The Arctic University of Norway, Tromsø, Norway. Department of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø, Norway.CAGE - Centre for Arctic Gas Hydrate, Environment and Climate, Department of Geosciences, UiT The Arctic University of Norway, Tromsø, Norway.Department of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø, Norway.Department of Microbiology, College of Sciences, Oregon State University, Corvallis, OR, USA.CAGE - Centre for Arctic Gas Hydrate, Environment and Climate, Department of Geosciences, UiT The Arctic University of Norway, Tromsø, Norway. Department of Marine Microbiology & Biogeochemistry, and Utrecht University, NIOZ Royal Netherlands Institute for Sea Research, 't Horntje, The Netherlands. Department of Earth Sciences, Faculty of Geosciences, Utrecht University, Utrecht, The Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31278352

Citation

Gründger, Friederike, et al. "Methane-fuelled Biofilms Predominantly Composed of Methanotrophic ANME-1 in Arctic Gas Hydrate-related Sediments." Scientific Reports, vol. 9, no. 1, 2019, p. 9725.
Gründger F, Carrier V, Svenning MM, et al. Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments. Sci Rep. 2019;9(1):9725.
Gründger, F., Carrier, V., Svenning, M. M., Panieri, G., Vonnahme, T. R., Klasek, S., & Niemann, H. (2019). Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments. Scientific Reports, 9(1), p. 9725. doi:10.1038/s41598-019-46209-5.
Gründger F, et al. Methane-fuelled Biofilms Predominantly Composed of Methanotrophic ANME-1 in Arctic Gas Hydrate-related Sediments. Sci Rep. 2019 Jul 5;9(1):9725. PubMed PMID: 31278352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments. AU - Gründger,Friederike, AU - Carrier,Vincent, AU - Svenning,Mette M, AU - Panieri,Giuliana, AU - Vonnahme,Tobias R, AU - Klasek,Scott, AU - Niemann,Helge, Y1 - 2019/07/05/ PY - 2019/02/07/received PY - 2019/06/25/accepted PY - 2019/7/7/entrez PY - 2019/7/7/pubmed PY - 2019/7/7/medline SP - 9725 EP - 9725 JF - Scientific reports JO - Sci Rep VL - 9 IS - 1 N2 - Sedimentary biofilms comprising microbial communities mediating the anaerobic oxidation of methane are rare. Here, we describe two biofilm communities discovered in sediment cores recovered from Arctic cold seep sites (gas hydrate pingos) in the north-western Barents Sea, characterized by steady methane fluxes. We found macroscopically visible biofilms in pockets in the sediment matrix at the depth of the sulphate-methane-transition zone. 16S rRNA gene surveys revealed that the microbial community in one of the two biofilms comprised exclusively of putative anaerobic methanotrophic archaea of which ANME-1 was the sole archaeal taxon. The bacterial community consisted of relatives of sulphate-reducing bacteria (SRB) belonging to uncultured Desulfobacteraceae clustering into SEEP-SRB1 (i.e. the typical SRB associated to ANME-1), and members of the atribacterial JS1 clade. Confocal laser scanning microscopy demonstrates that this biofilm is composed of multicellular strands and patches of ANME-1 that are loosely associated with SRB cells, but not tightly connected in aggregates. Our discovery of methanotrophic biofilms in sediment pockets closely associated with methane seeps constitutes a hitherto overlooked and potentially widespread sink for methane and sulphate in marine sediments. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/31278352/Methane-fuelled_biofilms_predominantly_composed_of_methanotrophic_ANME-1_in_Arctic_gas_hydrate-related_sediments L2 - http://dx.doi.org/10.1038/s41598-019-46209-5 DB - PRIME DP - Unbound Medicine ER -