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Fibrinolytic response and fibrin fragment D-dimer levels in patients with deep vein thrombosis.
Thromb Haemost. 1987 Dec 18; 58(4):1024-9.TH

Abstract

An enzyme-linked immunosorbent assay for fragment D-dimer was developed with the use of two monoclonal antibodies directed against specific non-overlapping antigenic determinants, present in fragment D-dimer of crosslinked fibrin but not in fragment D of non crosslinked fibrin or of fibrinogen. The lower limit of sensitivity of the assay when applied to human plasma, is 25 ng/ml. Concentration of fragment D-dimer in plasma from healthy individuals was 177 +/- 83 ng/ml (mean +/- SD). In plasma of 11 out of 12 patients with phlebographically confirmed acute deep vein thrombosis, fragment D-dimer levels were significantly increased. Fragment D-dimer was not increased in 9 out of 10 patients with recurrent idiopathic deep vein thrombosis during clinically silent episodes. Total t-PA antigen and free t-PA antigen concentrations were measured using previously developed ELISAs. Nine of the 12 patients with acute deep vein thrombosis showed a significant increase of total t-PA antigen (from 8.6 +/- 6.9 ng/ml to 21 +/- 16 ng/ml) after venous occlusion but in 3 of these free t-PA remained undetectable. Five of the 10 patients with recurrent deep vein thrombosis responded to venous occlusion with a significant increase of total t-PA antigen (from 6.7 +/- 3.2 ng/ml to 14 +/- 7.9 ng/ml) but, in all patients, free t-PA antigen remained undetectable. It is concluded that the combined assays of total and free t-PA antigen and of fragment D-dimer may be useful for the evaluation of the dynamics of the fibrinolytic system in physiological and pathological conditions.

Authors+Show Affiliations

Center for Thrombosis and Vascular Research, University of Leuven, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3127914

Citation

Declerck, P J., et al. "Fibrinolytic Response and Fibrin Fragment D-dimer Levels in Patients With Deep Vein Thrombosis." Thrombosis and Haemostasis, vol. 58, no. 4, 1987, pp. 1024-9.
Declerck PJ, Mombaerts P, Holvoet P, et al. Fibrinolytic response and fibrin fragment D-dimer levels in patients with deep vein thrombosis. Thromb Haemost. 1987;58(4):1024-9.
Declerck, P. J., Mombaerts, P., Holvoet, P., De Mol, M., & Collen, D. (1987). Fibrinolytic response and fibrin fragment D-dimer levels in patients with deep vein thrombosis. Thrombosis and Haemostasis, 58(4), 1024-9.
Declerck PJ, et al. Fibrinolytic Response and Fibrin Fragment D-dimer Levels in Patients With Deep Vein Thrombosis. Thromb Haemost. 1987 Dec 18;58(4):1024-9. PubMed PMID: 3127914.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fibrinolytic response and fibrin fragment D-dimer levels in patients with deep vein thrombosis. AU - Declerck,P J, AU - Mombaerts,P, AU - Holvoet,P, AU - De Mol,M, AU - Collen,D, PY - 1987/12/18/pubmed PY - 1987/12/18/medline PY - 1987/12/18/entrez SP - 1024 EP - 9 JF - Thrombosis and haemostasis JO - Thromb Haemost VL - 58 IS - 4 N2 - An enzyme-linked immunosorbent assay for fragment D-dimer was developed with the use of two monoclonal antibodies directed against specific non-overlapping antigenic determinants, present in fragment D-dimer of crosslinked fibrin but not in fragment D of non crosslinked fibrin or of fibrinogen. The lower limit of sensitivity of the assay when applied to human plasma, is 25 ng/ml. Concentration of fragment D-dimer in plasma from healthy individuals was 177 +/- 83 ng/ml (mean +/- SD). In plasma of 11 out of 12 patients with phlebographically confirmed acute deep vein thrombosis, fragment D-dimer levels were significantly increased. Fragment D-dimer was not increased in 9 out of 10 patients with recurrent idiopathic deep vein thrombosis during clinically silent episodes. Total t-PA antigen and free t-PA antigen concentrations were measured using previously developed ELISAs. Nine of the 12 patients with acute deep vein thrombosis showed a significant increase of total t-PA antigen (from 8.6 +/- 6.9 ng/ml to 21 +/- 16 ng/ml) after venous occlusion but in 3 of these free t-PA remained undetectable. Five of the 10 patients with recurrent deep vein thrombosis responded to venous occlusion with a significant increase of total t-PA antigen (from 6.7 +/- 3.2 ng/ml to 14 +/- 7.9 ng/ml) but, in all patients, free t-PA antigen remained undetectable. It is concluded that the combined assays of total and free t-PA antigen and of fragment D-dimer may be useful for the evaluation of the dynamics of the fibrinolytic system in physiological and pathological conditions. SN - 0340-6245 UR - https://www.unboundmedicine.com/medline/citation/3127914/Fibrinolytic_response_and_fibrin_fragment_D_dimer_levels_in_patients_with_deep_vein_thrombosis_ L2 - https://www.diseaseinfosearch.org/result/7087 DB - PRIME DP - Unbound Medicine ER -