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Toxicity mechanism of sevoflurane in neural stem cells of rats through DNA methylation.
Exp Ther Med 2019; 18(1):237-241ET

Abstract

The present study investigated the influence of sevoflurane on the cytotoxicity of neural stem cells of rats and deoxyribonucleic acid (DNA) methylation, and analyzed the correlation between degree of methylation and neurotoxicity of sevoflurane. Ten healthy Sprague-Dawley rats aged 6-8 weeks were randomly selected. The neural stem cells in the hippocampus of rats were isolated, followed by multiplication culture and induced differentiation. The nerve-related factors were observed and detected under a microscope. Moreover, the neural stem cells were treated with sevoflurane in different concentrations. Three wells were only added with the normal medium as the control group (C0), 3 wells were added with the low-concentration sevoflurane (0.2 g/ml) prepared by the medium as the low-concentration group (C1), 3 wells were added with the moderate-concentration of sevoflurane (0.5 g/ml) as the moderate-concentration group (C2), and 3 wells were added with the high-concentration sevoflurane (1 g/ml) as the high-concentration group (C3). The apoptosis rate was detected and calculated via Cell Counting Kit-8 (CCK-8) assay, the content of genomic DNA methylation in neural stem cells in each group was detected via high-performance liquid chromatography (HPLC), and the distribution of methylation in the chromosome in each group was compared. During the culture, neurospheres were produced, and the expression levels of four neural markers were increased. With the increase of sevoflurane concentration and the prolongation of time, the apoptosis rate of stem cells was increased. The content of methylation in cells treated with sevoflurane in a higher concentration was higher than that in other groups (P<0.05). According to the Pearsons correlation analysis, the content of methylation in neural stem cells was directly proportional to the concentration of sevoflurane. Methylation mostly occurred in the autosome, and the content of methylation in the high-concentration group was higher than those in the moderate-concentration, low-concentration and control groups (P<0.05). In conclusion, the concentration of sevoflurane can affect the degree of methylation in neural stem cells of rats and produce certain cytotoxicity.

Authors+Show Affiliations

Department of Anesthesiology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510280, P.R. China. Department of Anesthesiology, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou, Hainan 570208, P.R. China.Department of Anesthesiology, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou, Hainan 570208, P.R. China.Department of Central Laboratory, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou, Hainan 570208, P.R. China.Department of Anesthesiology, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou, Hainan 570208, P.R. China.Department of Anesthesiology, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou, Hainan 570208, P.R. China.Department of Anesthesiology, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou, Hainan 570208, P.R. China.Department of Anesthesiology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510280, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31281437

Citation

Wang, Ka, et al. "Toxicity Mechanism of Sevoflurane in Neural Stem Cells of Rats Through DNA Methylation." Experimental and Therapeutic Medicine, vol. 18, no. 1, 2019, pp. 237-241.
Wang K, Tian Y, Zhang Y, et al. Toxicity mechanism of sevoflurane in neural stem cells of rats through DNA methylation. Exp Ther Med. 2019;18(1):237-241.
Wang, K., Tian, Y., Zhang, Y., Li, X., Wei, X., Hu, H., & Xu, S. (2019). Toxicity mechanism of sevoflurane in neural stem cells of rats through DNA methylation. Experimental and Therapeutic Medicine, 18(1), pp. 237-241. doi:10.3892/etm.2019.7553.
Wang K, et al. Toxicity Mechanism of Sevoflurane in Neural Stem Cells of Rats Through DNA Methylation. Exp Ther Med. 2019;18(1):237-241. PubMed PMID: 31281437.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toxicity mechanism of sevoflurane in neural stem cells of rats through DNA methylation. AU - Wang,Ka, AU - Tian,Yi, AU - Zhang,Yingai, AU - Li,Xuying, AU - Wei,Xiao, AU - Hu,Haiyou, AU - Xu,Shiyuan, Y1 - 2019/05/08/ PY - 2018/11/22/received PY - 2019/03/26/accepted PY - 2019/7/9/entrez PY - 2019/7/10/pubmed PY - 2019/7/10/medline KW - DNA methylation KW - cytotoxicity KW - neural stem cells KW - rat KW - sevoflurane SP - 237 EP - 241 JF - Experimental and therapeutic medicine JO - Exp Ther Med VL - 18 IS - 1 N2 - The present study investigated the influence of sevoflurane on the cytotoxicity of neural stem cells of rats and deoxyribonucleic acid (DNA) methylation, and analyzed the correlation between degree of methylation and neurotoxicity of sevoflurane. Ten healthy Sprague-Dawley rats aged 6-8 weeks were randomly selected. The neural stem cells in the hippocampus of rats were isolated, followed by multiplication culture and induced differentiation. The nerve-related factors were observed and detected under a microscope. Moreover, the neural stem cells were treated with sevoflurane in different concentrations. Three wells were only added with the normal medium as the control group (C0), 3 wells were added with the low-concentration sevoflurane (0.2 g/ml) prepared by the medium as the low-concentration group (C1), 3 wells were added with the moderate-concentration of sevoflurane (0.5 g/ml) as the moderate-concentration group (C2), and 3 wells were added with the high-concentration sevoflurane (1 g/ml) as the high-concentration group (C3). The apoptosis rate was detected and calculated via Cell Counting Kit-8 (CCK-8) assay, the content of genomic DNA methylation in neural stem cells in each group was detected via high-performance liquid chromatography (HPLC), and the distribution of methylation in the chromosome in each group was compared. During the culture, neurospheres were produced, and the expression levels of four neural markers were increased. With the increase of sevoflurane concentration and the prolongation of time, the apoptosis rate of stem cells was increased. The content of methylation in cells treated with sevoflurane in a higher concentration was higher than that in other groups (P<0.05). According to the Pearsons correlation analysis, the content of methylation in neural stem cells was directly proportional to the concentration of sevoflurane. Methylation mostly occurred in the autosome, and the content of methylation in the high-concentration group was higher than those in the moderate-concentration, low-concentration and control groups (P<0.05). In conclusion, the concentration of sevoflurane can affect the degree of methylation in neural stem cells of rats and produce certain cytotoxicity. SN - 1792-0981 UR - https://www.unboundmedicine.com/medline/citation/31281437/Toxicity_mechanism_of_sevoflurane_in_neural_stem_cells_of_rats_through_DNA_methylation L2 - https://www.ingentaconnect.com/openurl?genre=article&amp;issn=1792-0981&amp;volume=18&amp;issue=1&amp;spage=237&amp;aulast=Wang DB - PRIME DP - Unbound Medicine ER -