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[Application of Intermediate Mesoderm-like Cells in Kidney Regeneration].

Abstract

Objective To induce adipose-derived stem cells (ADSCs) to differentiate into intermediate mesoderm (IM)-like cells in vitro,with IM-like cells for recellularizing kidney scaffolds,and then to obtain a tissue-engineering kidney with renal structures and functions through co-culture.Methods After inguinal fat pads of Wistar rats were surgically harvested,the primary ADSCs were isolated,induced,and cultured for stem cell identification. ADSCs were inducted to differentiate into IM-like cells by adding glycogen synthase kinase-3 inhibitor (CHIR99021) and fibroblast growth factor 9 (FGF9) at different stages. Seven days later,the IM-like cells were identified. The induced IM-like cells and well-prepared kidney decellularized scaffolds were co-cultured for 10 days to obtain recellularized tissue-engineered kidneys and their differentiation was identified.Results The ADSCs harvested had osteogenic and adipogenic abilities and could express the stem cell surface markers. After 7 days of in vitro induction,the positive expressions of odd-skipped related 1 and paired-box 2 were observed in IM-like cells by immunofluorescence technique. After 10 days of co-culture with kidney decellularized scaffolds,the positive expressions of Wilms'tumor 1,GATA-binding protein-3,and E-cadherin were observed by immunofluorescence technique.Conclusion ADSCs can be induced into IM-like cells,and renal cell differentiation can be observed through combining the induced IM-like cells with kidney decellularized scaffolds.

Authors+Show Affiliations

Department of Urology, the Second Hospital of Shandong University,Jinan 250033,China.Department of Urology, the Second Hospital of Shandong University,Jinan 250033,China.Department of Central Research Lab,the Second Hospital of Shandong University,Jinan 250033,China.Department of Urology,Shandong Provincial Hospital of Shandong University,Jinan 250012,China.

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

31282321

Citation

Zhang, Jian Ye, et al. "[Application of Intermediate Mesoderm-like Cells in Kidney Regeneration]." Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, vol. 41, no. 3, 2019, pp. 291-299.
Zhang JY, Guan Y, Kong F, et al. [Application of Intermediate Mesoderm-like Cells in Kidney Regeneration]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2019;41(3):291-299.
Zhang, J. Y., Guan, Y., Kong, F., & Zhao, S. T. (2019). [Application of Intermediate Mesoderm-like Cells in Kidney Regeneration]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, 41(3), pp. 291-299. doi:10.3881/j.issn.1000-503X.11157.
Zhang JY, et al. [Application of Intermediate Mesoderm-like Cells in Kidney Regeneration]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2019 Jun 30;41(3):291-299. PubMed PMID: 31282321.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Application of Intermediate Mesoderm-like Cells in Kidney Regeneration]. AU - Zhang,Jian Ye, AU - Guan,Yong, AU - Kong,Feng, AU - Zhao,Sheng Tian, PY - 2019/7/9/entrez SP - 291 EP - 299 JF - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae JO - Zhongguo Yi Xue Ke Xue Yuan Xue Bao VL - 41 IS - 3 N2 - Objective To induce adipose-derived stem cells (ADSCs) to differentiate into intermediate mesoderm (IM)-like cells in vitro,with IM-like cells for recellularizing kidney scaffolds,and then to obtain a tissue-engineering kidney with renal structures and functions through co-culture.Methods After inguinal fat pads of Wistar rats were surgically harvested,the primary ADSCs were isolated,induced,and cultured for stem cell identification. ADSCs were inducted to differentiate into IM-like cells by adding glycogen synthase kinase-3 inhibitor (CHIR99021) and fibroblast growth factor 9 (FGF9) at different stages. Seven days later,the IM-like cells were identified. The induced IM-like cells and well-prepared kidney decellularized scaffolds were co-cultured for 10 days to obtain recellularized tissue-engineered kidneys and their differentiation was identified.Results The ADSCs harvested had osteogenic and adipogenic abilities and could express the stem cell surface markers. After 7 days of in vitro induction,the positive expressions of odd-skipped related 1 and paired-box 2 were observed in IM-like cells by immunofluorescence technique. After 10 days of co-culture with kidney decellularized scaffolds,the positive expressions of Wilms'tumor 1,GATA-binding protein-3,and E-cadherin were observed by immunofluorescence technique.Conclusion ADSCs can be induced into IM-like cells,and renal cell differentiation can be observed through combining the induced IM-like cells with kidney decellularized scaffolds. SN - 1000-503X UR - https://www.unboundmedicine.com/medline/citation/31282321/[Application_of_Intermediate_Mesoderm-like_Cells_in_Kidney_Regeneration] L2 - http://www.actacams.com/article/2019/1000-503X/1000-503X-41-3-291.pdf DB - PRIME DP - Unbound Medicine ER -