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Phenotypic and proteomic characterization of treponemes associated with bovine digital dermatitis.
Vet Microbiol. 2019 Aug; 235:35-42.VM

Abstract

Bovine digital dermatitis (BDD) is a multifactorial polymicrobial infectious disease associated with multiple species and phylotypes of treponemes. However, despite the abundance of molecular signatures for treponemes that are identified in bovine lesions, relatively few isolates are cultured, and even fewer have been characterized at the level of protein expression. Here we report the successful isolation and characterization of novel strains of T. brennaborense and T. phagedenis from cases of BDD in Iowa dairy cows, and compare them to a well characterized strain of T. phagedenis, and the type strain of the more recently recognized T. pedis. Propagation of T. brennaborense was only possible at room temperature in Cooked Meat Medium, and not in oral treponeme enrichment medium at 37 °C as used for T. phagedenis and T. pedis. A prominent and rapid motility is observed by T. brennaborense under dark-field microscopy. The highly motile T. brennaborense strain 11-3 has an identical enzymatic profile to that of the only other isolate of T. brennaborense to be cultured from a lesion of BDD. Outer membrane protein profiles of each strain were compared by 2-D gel electrophoresis, and the five most abundant proteins in each strain were identified by mass spectrometry. All identified proteins are predicted to have signal peptides. Results identified outer membrane proteins specific to each strain including predicted membrane lipoproteins, ABC transporters and, as yet, uncharacterized proteins. Collectively, our results provide for the identification and characterization of outer membrane components of multiple phylotypes of treponemes associated with BDD which can facilitate development of vaccines and diagnostics in our efforts to eradicate the disease.

Authors+Show Affiliations

Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, USA. Electronic address: Jarlath.nally@usda.gov.Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, USA.Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, USA.The Pasarow Mass Spectrometry Laboratory, The Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31282377

Citation

Nally, Jarlath E., et al. "Phenotypic and Proteomic Characterization of Treponemes Associated With Bovine Digital Dermatitis." Veterinary Microbiology, vol. 235, 2019, pp. 35-42.
Nally JE, Hornsby RL, Alt DP, et al. Phenotypic and proteomic characterization of treponemes associated with bovine digital dermatitis. Vet Microbiol. 2019;235:35-42.
Nally, J. E., Hornsby, R. L., Alt, D. P., & Whitelegge, J. P. (2019). Phenotypic and proteomic characterization of treponemes associated with bovine digital dermatitis. Veterinary Microbiology, 235, 35-42. https://doi.org/10.1016/j.vetmic.2019.05.023
Nally JE, et al. Phenotypic and Proteomic Characterization of Treponemes Associated With Bovine Digital Dermatitis. Vet Microbiol. 2019;235:35-42. PubMed PMID: 31282377.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic and proteomic characterization of treponemes associated with bovine digital dermatitis. AU - Nally,Jarlath E, AU - Hornsby,Richard L, AU - Alt,David P, AU - Whitelegge,Julian P, Y1 - 2019/05/30/ PY - 2019/03/19/received PY - 2019/05/16/revised PY - 2019/05/24/accepted PY - 2019/7/10/pubmed PY - 2019/9/20/medline PY - 2019/7/9/entrez KW - Bovine digital dermatitis KW - Outer membrane proteins KW - Treponema SP - 35 EP - 42 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 235 N2 - Bovine digital dermatitis (BDD) is a multifactorial polymicrobial infectious disease associated with multiple species and phylotypes of treponemes. However, despite the abundance of molecular signatures for treponemes that are identified in bovine lesions, relatively few isolates are cultured, and even fewer have been characterized at the level of protein expression. Here we report the successful isolation and characterization of novel strains of T. brennaborense and T. phagedenis from cases of BDD in Iowa dairy cows, and compare them to a well characterized strain of T. phagedenis, and the type strain of the more recently recognized T. pedis. Propagation of T. brennaborense was only possible at room temperature in Cooked Meat Medium, and not in oral treponeme enrichment medium at 37 °C as used for T. phagedenis and T. pedis. A prominent and rapid motility is observed by T. brennaborense under dark-field microscopy. The highly motile T. brennaborense strain 11-3 has an identical enzymatic profile to that of the only other isolate of T. brennaborense to be cultured from a lesion of BDD. Outer membrane protein profiles of each strain were compared by 2-D gel electrophoresis, and the five most abundant proteins in each strain were identified by mass spectrometry. All identified proteins are predicted to have signal peptides. Results identified outer membrane proteins specific to each strain including predicted membrane lipoproteins, ABC transporters and, as yet, uncharacterized proteins. Collectively, our results provide for the identification and characterization of outer membrane components of multiple phylotypes of treponemes associated with BDD which can facilitate development of vaccines and diagnostics in our efforts to eradicate the disease. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/31282377/Phenotypic_and_proteomic_characterization_of_treponemes_associated_with_bovine_digital_dermatitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(19)30344-X DB - PRIME DP - Unbound Medicine ER -