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Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice.
J Vis Exp 2019; (148)JV

Abstract

In recent years, optogenetics has been widely used in many fields of neuroscientific research. In many cases, an opsin, such as channel rhodopsin 2 (ChR2), is expressed by a virus vector in a particular type of neuronal cells in various Cre-driver mice. Activation of these opsins is triggered by application of light pulses which are delivered by laser or LED through optic cables, and the effect of activation is observed with very high time resolution. Experimenters are able to acutely stimulate neurons while monitoring behavior or another physiological outcome in mice. Optogenetics can enable useful strategies to evaluate function of neuronal circuits in the regulation of sleep/wakefulness states in mice. Here we describe a technique for examining the effect of optogenetic manipulation of neurons with a specific chemical identity during electroencephalogram (EEG) and electromyogram (EMG) monitoring to evaluate the sleep stage of mice. As an example, we describe manipulation of GABAergic neurons in the bed nucleus of the stria terminalis (BNST). Acute optogenetic excitation of these neurons triggers a rapid transition to wakefulness when applied during NREM sleep. Optogenetic manipulation along with EEG/EMG recording can be applied to decipher the neuronal circuits that regulate sleep/wakefulness states.

Authors+Show Affiliations

Department of Molecular Neuroscience and Integrative Physiology, Faculty of Medicine, Kanazawa University.International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba.International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba; Faculty of Medicine, University of Tsukuba; sakurai.takeshi.gf@u.tsukuba.ac.jp.

Pub Type(s)

Journal Article
Video-Audio Media

Language

eng

PubMed ID

31282883

Citation

Kodani, Shota, et al. "Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice." Journal of Visualized Experiments : JoVE, 2019.
Kodani S, Soya S, Sakurai T. Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice. J Vis Exp. 2019.
Kodani, S., Soya, S., & Sakurai, T. (2019). Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice. Journal of Visualized Experiments : JoVE, (148), doi:10.3791/58613.
Kodani S, Soya S, Sakurai T. Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice. J Vis Exp. 2019 Jun 19;(148) PubMed PMID: 31282883.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice. AU - Kodani,Shota, AU - Soya,Shingo, AU - Sakurai,Takeshi, Y1 - 2019/06/19/ PY - 2019/7/9/entrez JF - Journal of visualized experiments : JoVE JO - J Vis Exp IS - 148 N2 - In recent years, optogenetics has been widely used in many fields of neuroscientific research. In many cases, an opsin, such as channel rhodopsin 2 (ChR2), is expressed by a virus vector in a particular type of neuronal cells in various Cre-driver mice. Activation of these opsins is triggered by application of light pulses which are delivered by laser or LED through optic cables, and the effect of activation is observed with very high time resolution. Experimenters are able to acutely stimulate neurons while monitoring behavior or another physiological outcome in mice. Optogenetics can enable useful strategies to evaluate function of neuronal circuits in the regulation of sleep/wakefulness states in mice. Here we describe a technique for examining the effect of optogenetic manipulation of neurons with a specific chemical identity during electroencephalogram (EEG) and electromyogram (EMG) monitoring to evaluate the sleep stage of mice. As an example, we describe manipulation of GABAergic neurons in the bed nucleus of the stria terminalis (BNST). Acute optogenetic excitation of these neurons triggers a rapid transition to wakefulness when applied during NREM sleep. Optogenetic manipulation along with EEG/EMG recording can be applied to decipher the neuronal circuits that regulate sleep/wakefulness states. SN - 1940-087X UR - https://www.unboundmedicine.com/medline/citation/31282883/Optogenetic_Manipulation_of_Neural_Circuits_During_Monitoring_Sleep/wakefulness_States_in_Mice L2 - https://dx.doi.org/10.3791/58613 DB - PRIME DP - Unbound Medicine ER -