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Determination of active vitamin B12 (cobalamin) in dietary supplements and ingredients by reversed-phase liquid chromatography: Single-laboratory validation.
Food Chem 2019; 298:125010FC

Abstract

Vitamin B12 dietary supplement can be critical to the alleviation strategies against micronutrient malnutrition and food insecurity. An HPLC-DAD method has been developed and validated, per AOAC SMPR 2016.017 (Standard Method Performance Requirements), for the quantitation of four bioactive forms of vitamin B12 (adenosylcobalamin, cyanocobalamin, hydroxocobalamin, methylcobalamin) from dietary ingredients and supplements. The method achieves chromatographic baseline resolution of vitamin B12 forms on a modern column platform without the expensive requirement of an ultra-high pressure liquid chromatography and/or mass spectrometry. The method has a wide analytical range (0.0005%w/w-85%w/w), high precision (reproducibility relative standard deviations ranged from 1.43% to 4.67%), and high accuracy (>96% spike recovery rate for 11 out of 12 accuracy testing data points). The method detection and quantification limits are less than 0.16 and 0.52 µg/mL, respectively. To our best knowledge, it is simpler, less time-consuming, and more economical than other published methods for its intended uses.

Authors+Show Affiliations

Eurofins Supplement Analysis Center, Eurofins Scientific, Inc., United States. Electronic address: xiaoqiu@eurofinsus.com.Eurofins Supplement Analysis Center, Eurofins Scientific, Inc., United States. Electronic address: haoshuzhang@eurofinsus.com.Eurofins Supplement Analysis Center, Eurofins Scientific, Inc., United States. Electronic address: yuhaoyin@eurofinsus.com.MilliporeSigma, United States. Electronic address: hillel.brandes@sial.com.MilliporeSigma, United States. Electronic address: teresa.marsala@sial.com.MilliporeSigma, United States. Electronic address: katherine.stenerson@sial.com.MilliporeSigma, United States. Electronic address: hugh.cramer@sial.com.Eurofins Supplement Analysis Center, Eurofins Scientific, Inc., United States. Electronic address: hongyou@eurofinsus.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31284091

Citation

Qiu, Xiao, et al. "Determination of Active Vitamin B12 (cobalamin) in Dietary Supplements and Ingredients By Reversed-phase Liquid Chromatography: Single-laboratory Validation." Food Chemistry, vol. 298, 2019, p. 125010.
Qiu X, Zhang H, Yin Y, et al. Determination of active vitamin B12 (cobalamin) in dietary supplements and ingredients by reversed-phase liquid chromatography: Single-laboratory validation. Food Chem. 2019;298:125010.
Qiu, X., Zhang, H., Yin, Y., Brandes, H., Marsala, T., Stenerson, K., ... You, H. (2019). Determination of active vitamin B12 (cobalamin) in dietary supplements and ingredients by reversed-phase liquid chromatography: Single-laboratory validation. Food Chemistry, 298, p. 125010. doi:10.1016/j.foodchem.2019.125010.
Qiu X, et al. Determination of Active Vitamin B12 (cobalamin) in Dietary Supplements and Ingredients By Reversed-phase Liquid Chromatography: Single-laboratory Validation. Food Chem. 2019 Nov 15;298:125010. PubMed PMID: 31284091.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of active vitamin B12 (cobalamin) in dietary supplements and ingredients by reversed-phase liquid chromatography: Single-laboratory validation. AU - Qiu,Xiao, AU - Zhang,Haoshu, AU - Yin,Yuhao, AU - Brandes,Hillel, AU - Marsala,Teresa, AU - Stenerson,Katherine, AU - Cramer,Hugh, AU - You,Hong, Y1 - 2019/06/14/ PY - 2018/10/31/received PY - 2019/05/08/revised PY - 2019/06/13/accepted PY - 2019/7/10/pubmed PY - 2019/7/10/medline PY - 2019/7/9/entrez KW - Adenosylcobalamin KW - Cobalamin KW - Cyanocobalamin KW - HPLC KW - Hydroxocobalamin KW - Methylcobalamin KW - Vitamin B12 SP - 125010 EP - 125010 JF - Food chemistry JO - Food Chem VL - 298 N2 - Vitamin B12 dietary supplement can be critical to the alleviation strategies against micronutrient malnutrition and food insecurity. An HPLC-DAD method has been developed and validated, per AOAC SMPR 2016.017 (Standard Method Performance Requirements), for the quantitation of four bioactive forms of vitamin B12 (adenosylcobalamin, cyanocobalamin, hydroxocobalamin, methylcobalamin) from dietary ingredients and supplements. The method achieves chromatographic baseline resolution of vitamin B12 forms on a modern column platform without the expensive requirement of an ultra-high pressure liquid chromatography and/or mass spectrometry. The method has a wide analytical range (0.0005%w/w-85%w/w), high precision (reproducibility relative standard deviations ranged from 1.43% to 4.67%), and high accuracy (>96% spike recovery rate for 11 out of 12 accuracy testing data points). The method detection and quantification limits are less than 0.16 and 0.52 µg/mL, respectively. To our best knowledge, it is simpler, less time-consuming, and more economical than other published methods for its intended uses. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/31284091/Determination_of_active_vitamin_B12_(cobalamin)_in_dietary_supplements_and_ingredients_by_reversed-phase_liquid_chromatography:_Single-laboratory_validation L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(19)31112-4 DB - PRIME DP - Unbound Medicine ER -