Tags

Type your tag names separated by a space and hit enter

Novel spectral manipulations for determinations of Tolnaftate along with related toxic compounds: Drug profiling and a comparative study.

Abstract

A comparative study using novel quadruple divisor and mean centering of ratio spectra spectrophotometric methods was developed for resolution of five- component mixture of Tolnaftate, β-naphthol (Tolnaftate alkaline degradation product and its toxic impurity), methyl(m-tolyl)carbamic acid (Tolnaftate alkaline degradation product), N-methyl-m-toluidine (Tolnaftate toxic impurity) and methyl paraben (as a preservative). For the novel quadruple divisor method, each component in the quinary mixture was determined by dividing the quinary mixture spectrum by a sum of standard spectrum of equal concentration of the other four components as a quadruple divisor. First derivative of each ratio spectra was then obtained which allowed selective determination of each component without interference from other components in the mixture. The second method was mean centering of ratio spectra that depended on utilizing the mean centered ratio spectra in four successive steps leading to enhancement of the signal to noise ratio. The absorption spectra of the five studied components were recorded in the wavelength range of 210-350 nm. The mean centered fourth ratio spectra amplitudes for each component were used for its determination. The developed methods were successfully applied for determination of laboratory prepared quinary mixtures to ensure method's specificity, then, were further applied on Tinea Cure® cream where no interference from excipients. For the first time, Tolnaftate was determined along with its toxic impurity; β-naphthol, that could be absorbed by the skin, causing systemic toxic effects, unlike Tolnaftate that poorly absorbed, indicating the significance of this work. The proposed methods were statistically compared with each other and with the reference method. Furthermore, ICH guidelines were followed for their validation.

Authors+Show Affiliations

Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmed Hegazy st., 62514 Beni-Suef, Egypt. Electronic address: raghdaemam88@gmail.com.Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmed Hegazy st., 62514 Beni-Suef, Egypt.Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmed Hegazy st., 62514 Beni-Suef, Egypt.Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmed Hegazy st., 62514 Beni-Suef, Egypt.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

31284238

Citation

Emam, Raghda A., et al. "Novel Spectral Manipulations for Determinations of Tolnaftate Along With Related Toxic Compounds: Drug Profiling and a Comparative Study." Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, vol. 223, 2019, p. 117290.
Emam RA, Abdelrahman MM, Abdelaleem EA, et al. Novel spectral manipulations for determinations of Tolnaftate along with related toxic compounds: Drug profiling and a comparative study. Spectrochim Acta A Mol Biomol Spectrosc. 2019;223:117290.
Emam, R. A., Abdelrahman, M. M., Abdelaleem, E. A., & Ali, N. W. (2019). Novel spectral manipulations for determinations of Tolnaftate along with related toxic compounds: Drug profiling and a comparative study. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 223, p. 117290. doi:10.1016/j.saa.2019.117290.
Emam RA, et al. Novel Spectral Manipulations for Determinations of Tolnaftate Along With Related Toxic Compounds: Drug Profiling and a Comparative Study. Spectrochim Acta A Mol Biomol Spectrosc. 2019 Dec 5;223:117290. PubMed PMID: 31284238.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel spectral manipulations for determinations of Tolnaftate along with related toxic compounds: Drug profiling and a comparative study. AU - Emam,Raghda A, AU - Abdelrahman,Maha M, AU - Abdelaleem,Eglal A, AU - Ali,Nouruddin W, Y1 - 2019/06/19/ PY - 2019/02/24/received PY - 2019/06/01/revised PY - 2019/06/17/accepted PY - 2019/7/10/pubmed PY - 2020/1/29/medline PY - 2019/7/9/entrez KW - Mean centering of ratio spectra KW - Methyl paraben KW - Quadruple divisor KW - Tolnaftate KW - |N-Methyl-m-toluidine KW - β-Naphthol SP - 117290 EP - 117290 JF - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JO - Spectrochim Acta A Mol Biomol Spectrosc VL - 223 N2 - A comparative study using novel quadruple divisor and mean centering of ratio spectra spectrophotometric methods was developed for resolution of five- component mixture of Tolnaftate, β-naphthol (Tolnaftate alkaline degradation product and its toxic impurity), methyl(m-tolyl)carbamic acid (Tolnaftate alkaline degradation product), N-methyl-m-toluidine (Tolnaftate toxic impurity) and methyl paraben (as a preservative). For the novel quadruple divisor method, each component in the quinary mixture was determined by dividing the quinary mixture spectrum by a sum of standard spectrum of equal concentration of the other four components as a quadruple divisor. First derivative of each ratio spectra was then obtained which allowed selective determination of each component without interference from other components in the mixture. The second method was mean centering of ratio spectra that depended on utilizing the mean centered ratio spectra in four successive steps leading to enhancement of the signal to noise ratio. The absorption spectra of the five studied components were recorded in the wavelength range of 210-350 nm. The mean centered fourth ratio spectra amplitudes for each component were used for its determination. The developed methods were successfully applied for determination of laboratory prepared quinary mixtures to ensure method's specificity, then, were further applied on Tinea Cure® cream where no interference from excipients. For the first time, Tolnaftate was determined along with its toxic impurity; β-naphthol, that could be absorbed by the skin, causing systemic toxic effects, unlike Tolnaftate that poorly absorbed, indicating the significance of this work. The proposed methods were statistically compared with each other and with the reference method. Furthermore, ICH guidelines were followed for their validation. SN - 1873-3557 UR - https://www.unboundmedicine.com/medline/citation/31284238/Novel_spectral_manipulations_for_determinations_of_Tolnaftate_along_with_related_toxic_compounds:_Drug_profiling_and_a_comparative_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-1425(19)30680-8 DB - PRIME DP - Unbound Medicine ER -